A5100410600- Genomics and Chromatin Dynamics
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Hereditary Neurological Disorders
- Ubiquitin and proteasome pathways
- Zebrafish Biomedical Research Applications
- RNA and protein synthesis mechanisms
- Cellular Mechanics and Interactions
- Histone Deacetylase Inhibitors Research
- Chemical Synthesis and Analysis
- Cellular transport and secretion
- Cholesterol and Lipid Metabolism
- Neurogenetic and Muscular Disorders Research
- Machine Learning in Bioinformatics
- Cancer, Lipids, and Metabolism
- Ferroptosis and cancer prognosis
- RNA regulation and disease
- Cancer-related gene regulation
Southern University of Science and Technology
2022-2024
First Affiliated Hospital of Jinan University
2021-2023
Wuhan University
2023
Renmin Hospital of Wuhan University
2023
Weifang Medical University
2023
Abstract Chromatin accessibility plays a critical role in the regulation of cell fate decisions. Although gene expression changes have been extensively profiled at single-cell level during early embryogenesis, dynamics chromatin cis-regulatory elements remain poorly studied. Here, we used plate-based ATAC-seq method to profile over 10 000 nuclei from zebrafish embryos. We investigated several important time points immediately after zygotic genome activation (ZGA), covering key developmental...
Abstract Bladder cancer (BCa) is one of the most common malignancies worldwide. However, lack accurate and effective targeted drugs has become a major problem in current clinical treatment BCa. Studies have demonstrated that squalene epoxidase (SQLE), as key rate-limiting enzyme cholesterol biosynthesis, involved development. In this study, our analysis The Cancer Genome Atlas, Genotype-Tissue Expression, Gene Expression Omnibus databases showed SQLE expression was significantly higher...
Summary Joint profiling of chromatin accessibility and gene expression from the same single cell/nucleus provides critical information about cell types in a tissue states during dynamic process. These emerging multi-omics techniques help investigation cell-type resolved regulatory mechanisms 1–7 . However, many methods are currently limited by low sensitivity, throughput or complex workflow. Here, we developed situ SHERRY after ATAC-seq (ISSAAC-seq), highly sensitive flexible method to...
Spastin, a microtubule-severing enzyme, is known to be important for neurite outgrowth. However, the role of spastin post-translational modification, particularly its phosphorylation regulation in neuronal outgrowth, remains unclear. This study aimed investigate effects eliminating on outgrowth rat hippocampal neurons. To accomplish this, we constructed mutant with eleven potential sites mutated alanine. The levels wildtype (WT) and (11A) were then detected using Phos-tag SDS-PAGE....
Abstract Chromatin accessibility plays a critical role in the regulation of cell fate decisions. Although gene expression changes have been extensively profiled at single-cell level during early embryogenesis, dynamics chromatin cis -regulatory elements remain poorly studied. Here, we used plate-based ATAC-seq method to profile over 10, 000 nuclei from zebrafish embryos. We investigated several important time points immediately after zygotic genome activation (ZGA), covering key...
Spastin is one of the proteins which lead to hereditary spastic paraplegia (HSP), whose dysfunction towards microtubule severing and membrane transporting critically important. The present study elucidate mechanisms protein stability regulation spastin. ubiquitin encoding plasmids are transfected into COS-7 cells with different fusion tags including Green Fluorescent Protein (GFP), mCherry Flag. expression level spastin was detected, activity neurite outgrowth were quantified. data showed...