The enzymatic components of the rabbit pulmonary monooxygenase system, cytochromes P-450I and P-450II NADPH-cytochrome P-450 reductase, are immunochemically distinct proteins. In microsomes, N-demethylation benzphetamine, amino-pyrine, ethylmorphine, O-deethylation 7-ethoxycoumarin dependent only on cytochrome P-450I, hydroxylation coumarin is apparently catalyzed by both cytochromes. Cytochrome from major forms hepatic induced phenobarbital or 3-methylcholanthrene, whereas indistinguishable...

Background: Alcohol abuse levels are very high in adolescents, creating a significant societal issue. It has been shown that people who begin alcohol use as adolescents more likely to become addicts than initiate adults. is important note the development of addiction humans rapid with initiation adolescence adulthood. Methods: To determine changes reinforcing efficacy function adolescent development, we used place‐conditioning paradigm. In this study, assessed ability ethanol support...

Cytochrome P450 metabolizes arachidonic acid to several unique and biologically active compounds in rabbit liver kidney. Microsomal fractions prepared from lung homogenates metabolized through cytochrome pathways, yielding cis-epoxyeicosatrienoic acids (EETs) their hydration products, vic-dihydroxyeicosatrienoic acids, mid-chain cis-trans conjugated dienols, 19- 20-hydroxyeicosatetraenoic acids. Inhibition studies using polyclonal antibodies against purified CYP2B4 demonstrated 100%...

Abstract Individuals who begin using alcohol prior to 14 years of age are 4 times more likely progress addiction than those do not initiate use until 21 age. The nucleus accumbens septi undergoes dramatic developmental transitions during the adolescent period, and dopaminergic activity within this region has been identified as a central neurochemical mediator drug reward, dependence. Thus, alcohol‐induced alterations in brain mediate heightened vulnerability observed users. To investigate...

Minocycline, a member of the tetracycline family, has low risk adverse effects and an ability to improve behavioral performance in humans with cognitive disruption. We performed single-arm open-label trial which 25 children diagnosed Angelman syndrome (AS) were administered minocycline assess safety tolerability this patient population determine drug's effect on manifestations disorder. Participants, age 4-12 years old, randomly selected from pool previously screened for participation study....

The currently accepted model for the membrane topology of microsomal cytochrome P450 is that a largely cytoplasmic domain bound by only one or two transmembrane segments at NH2 terminus. However, as we have reported previously, 2E1 lacking hydrophobic NH2-terminal signal peptide, like full-length protein, located in inner cell when expressed Escherichia coli and active with typical substrates. In present study, additional variants alcohol-inducible well truncated forms...

Enzyme components and activities of the cytochrome P-450 monooxygenase system in microsomal preparations from Clara cell, alveolar type II macrophage fractions isolated lungs untreated rabbits treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin were examined. Results are compared to those obtained whole lung. Concentrations isozymes 2 5 NADPH-cytochrome reductase higher cell than or For most part, however, differences among these 2-fold less. Microsomal fraction contained low undetectable...

The immunochemical relatedness, multiplicity, and expression of microsomal flavin-containing monooxygenases were assessed in hepatic, pulmonary, renal preparations from adult, male rabbits, mice, rats, guinea pigs, hamsters. Preparations adult female (pregnant nonpregnant) immature rabbits also examined. Microsomes analyzed by immunoblotting with polyclonal antibodies to purified pig, mouse, rabbit liver, lung. Pulmonary monooxygenases, which differ the major forms enzyme present detected...

Our studies have evaluated biochemical changes in placentae from humans exposed to rice oil contaminated with polychlorinated biphenyls (PCBs) and dibenzofurans (PCDFs) Taiwan. Placentae were obtained nonsmoking women 4 5 years after the exposure had occurred. The individuals ingested approximately 1 3 g PCBs mg PCDFs, many exhibited symptoms characteristic of PCB poisoning. This disease was termed "Yu-Cheng" Chinese. Based on data experimental animal models, we examined a number parameters...

Two forms of hepatic cytochrome P-450 have been separated and partially purified from untreated rabbits. The carbon monoxide-reduced minus reduced difference spectrum one form (A) had a peak absorption at 450.2 nm while the same other (B) gave 448 nm. Differences in spectral properties two were also seen Soret region spectra oxidized preparations, visible regions preparations complexed with monoxide, formed by addition type I II compounds. cytochromes to 6.5 14.3 nmoles/mg protein for A B,...

A microsomal fraction, prepared from mouse skin, catalyzed the hydroxylation of benzpyrene and aniline deethylation 7-ethoxycoumarin. Contamination preparation by cytochrome oxidase P-420 was determined spectral analysis. The enzyme activities studied in skin (Swiss-Webster CD-1) did not respond to topical application 3-MC. Twenty-four hours after TCDD mice, microsomes had 50% greater hydroxylase 7-ethoxycoumarin deethylase activity, 4- 8-fold activity these enzymes seen 72 hr. Increases...

Two full-length cDNA clones (2.2 kilobases) encoding a newly recognized form of mammalian flavin-containing monooxygenase (FMO) have been isolated from independent libraries constructed with mRNA different rabbits. The cDNAs encode polypeptide 533 amino acids which contains two putative pyrophosphate binding domains and hydrophobic carboxyl terminus characteristic FMOs. This sequence is 52 57% identical to sequences the rabbit hepatic pulmonary FMOs, respectively, 55% liver 2 published...

The nonciliated bronchiolar epithelial (Clara) cell of the mouse is highly susceptible to toxicants that undergo metabolic activation, presumably because this type has high levels cytochrome P-450 monooxygenases. As a first step in further defining role Clara cells pulmonary xenobiotic activation and detoxication, we have isolated (75 80% purity) characterized them morphologically biochemically. identity cells, confirmed by transmission electron microscopy, was based on several features,...

The presence of homologues rabbit cytochrome P-450 isozyme 5 in pulmonary and hepatic microsomal preparations from guinea pig, mouse, monkey, hamster, rat was examined by immunoblotting inhibition metabolism 2-aminofluorene with antibodies to 5. Homologues were detected all five species. However, only addition those rabbit, contained detectable levels this isozyme. With the exception induction phenobarbital liver, treatment animals or tetrachlorodibenzo-p-dioxin did not increase content...