Supramolecular hydrogels are used in the 3D printing of high-resolution, multi-material structures. The non-covalent bonds allow extrusion inks into support gels to directly write structures continuously space. This material system supports patterning multiple inks, cells, and void spaces.

The development of printable biomaterial inks is critical to the application 3D printing in biomedicine. To print high-resolution structures with fidelity a computer-aided design, materials used must be capable being deposited on surface and maintaining printed structure. A dual-cross-linking hyaluronic acid system was studied here as hydrogel ink, which encompassed both shear-thinning self-healing behaviors via guest–host bonding, well covalent cross-linking for stabilization using...

An in situ crosslinking strategy is used for 3D bioprinting of nonviscous photo-crosslinkable hydrogels. This method can be generalized to various formulations, maintaining high embedded cell viability and tunable behavior. Heterogeneous hollow filaments printed using this strategy, allowing fabrication complex engineered cell-laden constructs.

Abstract 3D printing involves the development of inks that exhibit requisite properties for both and intended application. In bioprinting, these are often hydrogels with controlled rheological can be stabilized after deposition. Here, an alternate approach is developed where ink composed exclusively jammed microgels, which designed to incorporate a range through microgel design (e.g., composition, size) mixing microgels. The shear‐thinning permit flow rapidly recover upon deposition,...

Double-network theory is extended to include guest-host interactions, enabling injectability and cytcompatibility of tough hydrogels. Noncovalent interactions are used as a sacrificial network toughen covalently crosslinked hydrogels formed from hyaluronic acid. Shear thinning supramolecular bonds allows hydrogel injection rapid self-healing, while gentle reaction conditions permit cell encapsulation with high viability.

The fabrication of three-dimensional (3D) scaffolds is indispensable to tissue engineering and 3D printing emerging as an important approach towards this. Hydrogels are often used inks in extrusion-based printing, including with encapsulated cells; however, numerous challenging requirements exist, appropriate viscosity, the ability stabilize after extrusion, cytocompatibility. Here, we present a shear-thinning self-healing hydrogel crosslinked through dynamic covalent chemistry for...

Abstract 3D‐printing is emerging as a technology to introduce microchannels into hydrogels, for the perfusion of engineered constructs. Although numerous techniques have been developed, new are still needed obtain complex geometries blood vessels and with materials that permit desired cellular responses. Here, printing process where shear‐thinning self‐healing hydrogel “ink” injected directly “support” similar properties reported. The support further undergo stabilization through thiol‐ene...

We reexamined the cellular drug delivery mechanism by poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) to determine their utility and limitations as an intracellular system. First, we prepared PLGA NPs which physically encapsulated Nile red (a hydrophobic fluorescent dye), in accordance with usual procedure for labeling NPs, incubated them mesothelial cells, observed increase fluorescence. then from chemically conjugated a dye uptake cells using confocal microscopy. also used coherent...

To develop a combined barrier method and drug delivery system ("hybrid system") for preventing postoperative peritoneal adhesions, which could combine the biocompatibility ease of application in situ cross-linkable hydrogels with controlled release features polymeric nanoparticles.Poly(lactic-co-glycolic acid) nanoparticles were dispersed aldehyde- hydrazide-modified hyaluronic acids (HA), then via double-barreled syringe. The material was subjected to mechanical testing assayed vitro...

Three-dimensional (3D) printed scaffolds have great potential in biomedicine; however, it is important that we are able to design such with a range of diverse properties towards specific applications. Here, report the extrusion-based 3D printing biodegradable and photocurable acrylated polyglycerol sebacate (Acr-PGS) fabricate elastic properties. Two Acr-PGS macromers were synthesized varied molecular weights viscosity, which then blended obtain macromer inks viscosities. The quality...

Polyelectrolyte microcapsules represent versatile stimuli-responsive structures that enable the encapsulation, protection, and release of active agents. Their conventional preparation methods, however, tend to be time-consuming, yield low encapsulation efficiency, seldom allow for dual incorporation hydrophilic hydrophobic materials, limiting their widespread utilization. In this work, we present a method fabricate polyelectrolyte in one step based on nanoscale interfacial complexation...

Photocurable norbornene-functionalized poly(glycerol sebacate) (Nor-PGS) has been developed to fabricate cytocompatible, biodegradable and elastomeric scaffolds for tissue engineering applications.

The fibrous architecture of the extracellular matrix (ECM) is recognized as an integral regulator cell function. However, there unmet need to develop mechanically robust biomaterials mimicking nanofibrous tissue topography that are also injectable enable minimally invasive delivery. In this study, we have developed a hydrogel composed supramolecularly assembled hyaluronic acid (HA) nanofibers exhibits mechanical integrity, shear-thinning behavior, rapid self-healing, and cytocompatibility....

Abstract The suitability of chitosan and UV‐cross‐linkable for intraperitoneal use, example as a barrier device preventing peritoneal adhesions or drug delivery, was examined. In vitro experiments using two major cell types present in the cavity (mesothelial cells macrophages) revealed neither attractive interactions between cross‐linked gels nor proliferative effect. However, same UV‐cross‐linked applied rabbits caused granulomatous reaction with adhesion formation within weeks all animals,...

Abstract Incorporation of photoresponsive molecules within soft materials can provide spatiotemporal control over bulk properties and address challenges in targeted delivery mechanical variability. However, the kinetics situ photochemical reactions are often slow typically employ ultraviolet wavelengths. Here, we present a novel photoactive crosslinker Ru(bipyridine) 2 (3‐pyridinaldehyde) (RuAldehyde), which was reacted with hydrazide‐functionalized hyaluronic acid to form hydrogels capable...

Microporous annealed particle (MAP) scaffolds consist of a slurry hydrogel microspheres that undergo annealing to form solid scaffold. MAP have contained functional groups with dual abilities participate in Michael-type addition (gelation) and radical polymerization (photoannealing). Functional efficient additions react thiols amines under physiological conditions, limiting usage for therapeutic delivery. We present heterofunctional maleimide/methacrylamide 4-arm PEG macromer (MethMal)...

Micro- and nanoencapsulation of cells has been studied as a strategy to protect from environmental stress promote survival during delivery. Hydrogels used in encapsulation can be modified influence cell behaviors direct assembly their surroundings. Here, we report system that conformally encapsulated stem using hyaluronic acid (HA). We successfully HA with lipid, thiol, maleimide pendant groups facilitate hydrogel which was deposited onto plasma membranes subsequently crosslinked through...