A5077982600- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Additive Manufacturing and 3D Printing Technologies
- Osteoarthritis Treatment and Mechanisms
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Periodontal Regeneration and Treatments
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- Knee injuries and reconstruction techniques
- Hydrogels: synthesis, properties, applications
- Animal testing and alternatives
- Advanced Machining and Optimization Techniques
- Cancer, Hypoxia, and Metabolism
- Total Knee Arthroplasty Outcomes
- Dental Implant Techniques and Outcomes
- Manufacturing Process and Optimization
Ollscoil na Gaillimhe – University of Galway
2022-2025
University Hospital Galway
2022-2025
Science Foundation Ireland
2023
University of Pennsylvania
2018-2021
Trinity College Dublin
2015-2019
Royal College of Surgeons in Ireland
2016-2018
Advanced Materials and BioEngineering Research
2016
Cartilage is a dense connective tissue with limited self-repair capabilities. Mesenchymal stem cell (MSC) laden hydrogels are commonly used for fibrocartilage and articular cartilage engineering, however they typically lack the mechanical integrity implantation into high load bearing environments. This has led to increased interested in 3D bioprinting of hydrogel bioinks reinforced stiffer polymer fibres. The objective this study was compare range (agarose, alginate, GelMA BioINK™) their...
Abstract 3D printing involves the development of inks that exhibit requisite properties for both and intended application. In bioprinting, these are often hydrogels with controlled rheological can be stabilized after deposition. Here, an alternate approach is developed where ink composed exclusively jammed microgels, which designed to incorporate a range through microgel design (e.g., composition, size) mixing microgels. The shear‐thinning permit flow rapidly recover upon deposition,...
Abstract Cellular models are needed to study human development and disease in vitro, screen drugs for toxicity efficacy. Current approaches limited the engineering of functional tissue with requisite cell densities heterogeneity appropriately model behaviors. Here, we develop a bioprinting approach transfer spheroids into self-healing support hydrogels at high resolution, which enables their patterning fusion high-cell density microtissues prescribed spatial organization. As an example...
The ability to print defined patterns of cells and extracellular-matrix components in three dimensions has enabled the engineering simple biological tissues; however, bioprinting functional solid organs is beyond capabilities current biofabrication technologies. An alternative approach would be bioprint developmental precursor an adult organ, using this engineered rudiment as a template for subsequent organogenesis vivo. This study demonstrates that developmentally inspired hypertrophic...
Abstract Many cell types require direct cell–cell interactions for differentiation and function; yet, this can be challenging to incorporate into 3‐dimensional (3D) structures the engineering of tissues. Here, a new approach is introduced that combines aggregates cells (spheroids) with similarly‐sized hydrogel particles (microgels) form granular composites are injectable, undergo interparticle crosslinking via light initial stabilization, permit contacts signaling, allow spheroid fusion...
Regeneration of complex bone defects remains a significant clinical challenge. Multi-tool biofabrication has permitted the combination various biomaterials to create multifaceted composites with tailorable mechanical properties and spatially controlled biological function. In this study we sought use bioprinting engineer nonviral gene activated constructs reinforced by polymeric micro-filaments. A bioink was developed using RGD-γ-irradiated alginate nano-hydroxyapatite (nHA) complexed...
Abstract Despite significant advances in bioprinting technology, current hardware platforms lack the capability for process monitoring and quality control. This limitation hampers translation of technology into industrial GMP-compliant manufacturing settings. As a key step towards solution, we developed novel platform integrating high-resolution camera in-situ extrusion outcomes during embedded bioprinting. Leveraging classical computer vision image analysis techniques, then created custom...
Mesenchymal stem cells maintained in appropriate culture conditions are capable of producing robust cartilage tissue. However, gradients nutrient availability that arise during three-dimensional can result the development spatially inhomogeneous tissues with core regions devoid matrix. Previous attempts at developing dynamic systems to overcome these limitations have reported suppression mesenchymal cell chondrogenesis compared static conditions. We hypothesize by modulating oxygen...
Abstract During embryogenesis, organs undergo dynamic shape transformations that sculpt their final shape, composition, and function. Despite this, current organ bioprinting approaches typically employ bioinks restrict cell‐generated morphogenetic behaviors resulting in structurally static tissues. This work introduces a novel platform enables the of tissues programmable predictable 4D shape‐morphing driven by forces. method utilizes embedded to deposit collagen‐hyaluronic acid within...
Abstract Extrusion bioprinting technology suffers from reproducibility challenges due to the open‐loop nature of current hardware systems. Here, a novel AI‐powered extrusion platform is presented with integrated real‐time quality monitoring and automated error correction capabilities. To achieve this, custom system engineered an camera for continuous process trained convolutional neural networks (CNNs) classify in real‐time. The CNN models, including Xception ResNet, are on combination real...
Abstract During embryogenesis, organs undergo dynamic shape transformations that sculpt their final shape, composition, and function. Despite this, current organ bioprinting approaches typically employ bioinks restrict cell-generated morphogenetic behaviours resulting in structurally static tissues. Here, we introduce a novel platform enables the of tissues programmable predictable 4D shape-morphing driven by forces. Our method utilises embedded to deposit collagen-hyaluronic acid within...
Abstract Cellular models are needed to study human development and disease in vitro , including the screening of drugs for toxicity efficacy. However, current approaches limited engineering functional tissue with requisite cell densities heterogeneity appropriately model behaviors. Here, we develop a new bioprinting approach transfer spheroids into self-healing support hydrogels at high resolution, which enables their patterning fusion high-cell density microtissues prescribed spatial...