A5033822755- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Forensic and Genetic Research
- Advanced biosensing and bioanalysis techniques
- Diptera species taxonomy and behavior
- Molecular Biology Techniques and Applications
- Insect symbiosis and bacterial influences
- COVID-19 diagnosis using AI
- Genomics and Phylogenetic Studies
- SARS-CoV-2 and COVID-19 Research
- Archaeology and ancient environmental studies
- Forensic Anthropology and Bioarchaeology Studies
- Venomous Animal Envenomation and Studies
- Statistical Methods and Inference
- Lepidoptera: Biology and Taxonomy
- Insect and Pesticide Research
Max Planck Institute for Evolutionary Anthropology
2019-2021
Max Planck Society
2021
Abstract Efforts to contain the spread of SARS-CoV-2 have spurred need for reliable, rapid, and cost-effective diagnostic methods which can be applied large numbers people. However, current standard protocols detection viral nucleic acids while sensitive, require a high level automation sophisticated laboratory equipment achieve throughputs that allow whole communities tested on regular basis. Here we present Cap-iLAMP (capture improved loop-mediated isothermal amplification) combines...
The Forbes’ Quarry and Devil’s Tower partial crania from Gibraltar are among the first Neanderthal remains ever found. Here, we show that small amounts of ancient DNA preserved in petrous bones 2 individuals despite unfavorable climatic conditions. However, endogenous is present an overwhelming excess recent human DNA. Using improved library construction methods enrich for fragments carrying deaminated cytosine residues, were able to sequence 70 0.4 megabase pairs (Mbp) nuclear specimens,...
Venoms and the toxins they contain represent molecular adaptations that have evolved on numerous occasions throughout animal kingdom. However, processes shape venom protein evolution are poorly understood because of scarcity whole-genome data available for comparative analyses venomous species.We performed a broad toxicogenomic analysis to gain insight into genomic mechanisms in robber flies (Asilidae). We first sequenced high-quality draft genome hymenopteran hunting fly Dasypogon diadema,...
SARS-CoV-2 causes substantial morbidity and mortality in elderly immunocompromised individuals, particularly retirement homes, where transmission from asymptomatic staff visitors may introduce the infection. Here we present a cheap fast screening method based on direct RT-qPCR to detect single or pooled gargle lavages ("mouthwashes"). This detects individuals with large viral loads (Ct≤29) use it test all at nursing home daily over period of three weeks order reduce risk that infection...
Extensive manipulations involved in the preparation of DNA samples for sequencing have hitherto made it impossible to determine precise structure double-stranded fragments being sequenced, such as presence blunt ends, single-stranded overhangs, or single-strand breaks. We here describe MatchSeq, a method that combines library from diluted with computational sequence matching, allowing reconstruction on single-molecule level. The application MatchSeq Neanderthal DNA, particularly complex...
Abstract SARS-CoV-2 causes substantial morbidity and mortality in elderly immunocompromised individuals, particularly retirement homes, where transmission from asymptomatic staff visitors may introduce the infection. Here we present a cheap fast approach to detect single or pooled gargle lavages (“mouthwashes”). With this approach, test all at nursing home daily over period of three weeks order reduce risk that infection penetrates facility. This similar approaches could be implemented...
Abstract Here we present a step-by-step protocol for Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines hybridization capture-based RNA extraction of gargle lavage samples with an colorimetric RT-LAMP assay smartphone-based color scoring. is compatible point-of-care testing enables the detection SARS-CoV-2 positive in less than one hour. The sensitivity 97% specificity 99%. In contrast to direct addition sample LAMP (iLAMP), prevents false positives...
Abstract Efforts to contain the spread of SARS-CoV-2 have spurred need for reliable, rapid, and cost-effective diagnostic methods which can easily be applied large numbers people. However, current standard protocols detection viral nucleic acids while sensitive, require a high level automation, sophisticated laboratory equipment trained personnel achieve throughputs that allow whole communities tested on regular basis. Here we present Cap-iLAMP (capture improved loop-mediated isothermal...
The complete mitochondrial genome of Dasypogon diadema (Insecta: Diptera) was sequenced using the Illumina MiSeq` platform. Its mt-genome spans over 16,947 bp with a GC content 26.6% containing 13 protein-coding genes, 22 tRNA and 2 rRNA genes. phylogenetic relationship 11 other dipteran species reconstructed position confirmed.