A5040657261- Immune Response and Inflammation
- Advanced Glycation End Products research
- Pancreatic function and diabetes
- Neuropeptides and Animal Physiology
- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Connexins and lens biology
- Neurological Disorders and Treatments
- Diabetes and associated disorders
- Receptor Mechanisms and Signaling
- Regulation of Appetite and Obesity
- Inflammasome and immune disorders
- Heme Oxygenase-1 and Carbon Monoxide
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Pain Mechanisms and Treatments
- S100 Proteins and Annexins
- Thermal Regulation in Medicine
- Electrochemical sensors and biosensors
- Cardiac Ischemia and Reperfusion
- SARS-CoV-2 and COVID-19 Research
- Cancer, Hypoxia, and Metabolism
- Genomics, phytochemicals, and oxidative stress
- Cell death mechanisms and regulation
- Lipid Membrane Structure and Behavior
Feinstein Institute for Medical Research
2011-2024
Donald & Barbara Zucker School of Medicine at Hofstra/Northwell
2020-2024
Northwell Health
2004-2024
North Shore University Hospital
2008-2018
Hofstra University
2014
Qingdao University
1999-2013
Chengdu University
2013
National Institute for Occupational Safety and Health
2013
Centers for Disease Control and Prevention
2013
Long Island Jewish Medical Center
2008-2011
Despite significant advances in intensive care therapy and antibiotics, severe sepsis accounts for 9% of all deaths the United States annually. The pathological sequelae are characterized by a systemic inflammatory response, but experimental therapeutics that target specific early mediators [tumor necrosis factor (TNF) IL-1beta] have not proven efficacious clinic. We recently identified high mobility group box 1 (HMGB1) as late mediator endotoxin-induced lethality exhibits significantly...
High mobility group box 1 (HMGB), a ubiquitous DNA-binding protein, has been implicated as proinflammatory cytokine and late mediator of lethal endotoxemia. HMGB1 is released by activated macrophages. It amplifies extends the inflammatory response inducing release mediating acute lung injury, anorexia, to tissue necrosis. The kinetics provide wide therapeutic window for endotoxemia because extracellular levels begin increase 12 24 h after exposure stimuli. Here, we demonstrate that domain...
Fetuin inhibits insulin-induced insulin receptor (IR) autophosphorylation and tyrosine kinase activity in vitro, intact cells, vivo. The fetuin gene (AHSG) is located on human chromosome 3q27, recently identified as a susceptibility locus for type 2 diabetes the metabolic syndrome. Here, we explore signaling, glucose homeostasis, effect of high-fat diet weight gain, body fat composition, disposal mice carrying two null alleles encoding fetuin, Ahsg (B6, 129-Ahsgtm1Mbl). knockout (KO)...
Abstract Bacterial endotoxin [lipopolysaccharide (LPS)] stimulates macrophages to sequentially release early [tumor necrosis factor (TNF)] and late [high mobility group box 1 (HMGB1)] proinflammatory cytokines. The requirement of CD14 mitogen-activated protein kinases [MAPK; e.g., p38 extracellular signal-regulated kinase (ERK)1/2] for endotoxin-induced TNF production has been demonstrated previously, but little is known about their involvement in endotoxin-mediated HMGB1 release. Here, we...
Stearoyl lysophosphatidylcholine (LPC) has recently been proven protective against lethal sepsis by stimulating neutrophils to eliminate invading pathogens through an H2O2-dependent mechanism. Here, we demonstrate that stearoyl LPC, but not caproyl significantly attenuates circulating high-mobility group box 1 (HMGB1) levels in endotoxemia and suppressing endotoxin-induced HMGB1 release from macrophages/monocytes. Neutralizing antibodies G2A, a potential cell surface receptor for partially...
Objectives: We have recently shown that ghrelin, a novel orexigenic hormone, is reduced in sepsis. Ghrelin treatment, mediated through ghrelin receptors the brain, attenuates sepsis-induced inflammation and mortality. Gut barrier dysfunction common High-mobility group B1 (HMGB1) increases gut permeability both vitro vivo. However, it remains unknown whether has any effects on HMGB1 function hypothesized decreases release central receptors. Design: Prospective, controlled, randomized animal...
Cytoplasmic membrane-bound connexin 43 (Cx43) proteins oligomerize into hexameric channels (hemichannels) that can sometimes dock with hemichannels on adjacent cells to form gap junctional (GJ) channels. However, the possible role of Cx43 in sterile and infectious inflammatory diseases has not been adequately defined due lack selective interventions. Here we report a proinflammatory mediator, serum amyloid A (SAA), resembled bacterial endotoxin by stimulating macrophages up-regulate...
An antibody strategy prevents harmful TN/HMGB1 interaction and resultant macrophage pyroptosis immunosuppression in sepsis.
Antibody-based strategies have been attempted to antagonize early cytokines of sepsis, but not yet tried target inducible late-acting mediators. Here, we report that the expression and secretion procathepsin-L (pCTS-L) was induced by serum amyloid A (SAA) in innate immune cells, contributing its late systemic accumulation experimental clinical sepsis. Recombinant pCTS-L interleukin-6 (IL-6), IL-8, GRO-α/KC, GRO-β/MIP-2, MCP-1 release cells moderately correlated with blood concentrations...
High glucose-induced oxidative stress is a major contributing mechanism to the development of diabetic cardiomyopathy. Nrf2 an emerging critical regulator cellular defense against damage. The role in cardiomyopathy was investigated vivo. Streptozotocin (STZ) induced diabetes knockout (KO) mice that rapidly progressed severe conditions with high mortality within two weeks injection; whereas, wild type (WT) mice, less no death. Severe myocardial lesions were observed KO had high, sublethal...
We have recently reported an important role of Connexin 43 (Cx43) hemichannels in the pathogenesis lethal sepsis through facilitating ATP efflux to potentiate double-stranded RNA-activated protein kinase R (PKR)-dependent macrophage activation. Here we further elucidated possible Pannexin 1 (Panx1) hemichannel by assessing its expression along with impact a Panx1-specific mimetic inhibitory peptide, 10Panx, on activity vitro and animal lethality vivo. Both crude bacterial lipopolysaccharide...
To determine whether administration of a vasoactive peptide, human adrenomedullin (AM), in combination with its binding protein (ie, AMBP-1), prevents or minimizes hepatic ischemia-reperfusion (I/R) injury.Hepatic I/R injury results from tissue hypoxia and subsequent inflammatory responses. Even though numerous pharmacological modalities substances have been studied to reduce I/R-induced mortality, none entirely successful. We shown that AM/AMBP-1 produces significant beneficial effects...