A total of 133 Listeria monocytogenes isolates were characterized by ribotyping and allelic analysis the virulence genes hly, actA, inlA to uncover linkages between independent phylogenetic specific markers. PCR-restriction fragment length polymorphisms revealed 8 11 inl4, 2 actA alleles. The combination these gene alleles ribotype patterns separated L. into three distinct lineages. While hly generally found cluster lineages, segregated independently. These lineages confirmed when 22 partial...

We report, for the first time, extensive biologically-mediated phosphate removal from wastewater during high-rate anaerobic digestion (AD). A hybrid sludge bed/fixed-film (packed pumice stone) reactor was employed low-temperature (12°C) treatment of synthetic sewage wastewater. Successful (up to 78% influent phosphate) observed, mediated by biofilms in reactor. Scanning electron microscopy and energy dispersive X-ray analysis revealed accumulation elemental phosphorus (~2%) within bed...

Fats, oil and grease, their hydrolyzed counterparts-long chain fatty acids (LCFA) make up a large fraction of numerous wastewaters are challenging to degrade anaerobically, more so, in low temperature anaerobic digestion (LtAD) systems. Herein, we perform comparative analysis publicly available Illumina 16S rRNA datasets generated from LCFA-degrading microbiomes at temperatures (10 20 °C) comprehend the factors affecting microbial community dynamics. The various considered were inoculum,...

The development and activity of a cold-adapting microbial community was monitored during low-temperature anaerobic digestion (LtAD) treatment wastewater. Two replicate hybrid sludge bed-fixed-film reactors treated synthetic sewage wastewater at 12°C, organic loading rates 0.25–1.0 kg chemical oxygen demand (COD) m−3 d−1, over 889 days. inoculum obtained from full-scale reactor, which operated 37°C. Both LtAD readily degraded the influent with COD removal efficiencies regularly exceeding 78%...

Pakistan is a multi-ethnic society where there disparity between dietary habits, genetic composition, and environmental exposures. The microbial ecology of healthy Pakistani gut in the context anthropometric, sociodemographic, patterns holds interest by virtue it being one most populous countries, also Lower Middle Income Country (LMIC). 16S rRNA profiling microbiome normo-weight individuals from different regions residence performed with additional meta-data collected through filled...

Abstract Background Aquaculture successfully meets global food demands for many fish species. However, aquaculture production of Atlantic cod ( Gadus morhua ) is just 2.5% total market production. For farming to be a viable economic venture specific challenges on how increase growth, health and productivity need addressed. Feed ingredients play key role here. Macroalgae (seaweeds) have been suggested as functional feed supplement with both benefits terrestrial farmed animals fish. The impact...

ABSTRACT SigB is the main stress gene regulator in Listeria monocytogenes affecting expression of more than 150 genes and thus contributing to multiple-stress resistance. Despite its clear role most stresses, oxidative uncertain, as results accompanying loss sigB range from hyperresistance hypersensitivity. Previously, these differences have been attributed strain variation. In this study, we show conclusively that unlike for all other H 2 O (more 8 log CFU ml −1 compared wild type)...

Farmers near towns and cities are using a wide range of highly polluted wastewaters for crop irrigation in Pakistan due to severe freshwater shortage. The present study aimed promote indigenous bacterial strains isolated from domestic, hospital, textile, pharmaceutical mixed remove contaminants colour render these safer irrigation. Thirty seven were five wastewater samples collected different sites Lahore, Pakistan. Under optimal growth conditions, three isolates (D6, D7 P1) showed >93%...

SPECIALTY GRAND CHALLENGE article Front. Syst. Biol., 17 June 2024Sec. Integrative Systems Microbiology Volume 4 - 2024 | https://doi.org/10.3389/fsysb.2024.1432791

Antimicrobial resistance (AMR) is a major global public health concern mainly affecting low- and middle-income countries (LMICs) due to lack of awareness, inadequate healthcare sanitation infrastructure, other environmental factors. In this study, we aimed link microbial assembly covariates (body mass index, smoking, use antibiotics) gut microbiome structure correlate the predictive antimicrobial gene prevalence (piARG) using PICRUSt2. We examined gastrointestinal oral profiles healthy...

Abstract Hospital wastewaters are produced in large volumes Pakistan (∼362–745 L/bed.day) and discharged without proper treatment. They widely used by farmers for crop irrigation induce a phytotoxic effect on plant growth. The study was conducted to evaluate the of untreated treated hospital wastewater seed germination fodder Trifolium alexandrinum (Berseem clover) food Solanum lycopersicum (tomato). A bacterial consortium formed with three strains, i.e., Alcaligenes faecalis Bacillus...

Septic tanks are widely deployed for off-grid sewage management but typified by poor treatment performance, discharge of polluting effluents and the requirement frequent de-sludging. The Solar Tank (SST) is a novel septic tank design that uses passive heat from sun to raise in-tank temperatures improves solids degradation, resulting in cleaner effluent. Treatment has been shown exceed conventional systems, however, underlying biology driving system poorly understood. We used next generation...

The structure of biofilms in drinking water systems is influenced by the interplay between biological and physical processes. Bacterial aggregates bulk fluid are important seeding biofilm formation on surfaces. In simple pure co-cultures, certain bacteria, including Methylobacterium, implicated aggregates. However, it unclear whether they help to form complex mixed bacterial communities. Furthermore, different flow regimes could affect destination this study, real microbial communities were...

In this study, the ability of various sludges to digest a diverse range cellulose and cellulose-derived substrates was assessed at different temperatures elucidate factors affecting hydrolysis. For purpose, biogas production monitored specific activity (SBA) employed compare performance three anaerobic on degradation variety complex sources, across temperatures. The sludge with highest derived from full-scale bioreactor treating sewage 37 °C. Hydrolysis rate-limiting step during substrates....

This article presents metagenomic-assembled genomes (MAGs) of prokaryotic organisms originating from chicken caeca. The samples originate broiler chickens, one group was infected with Newcastle Disease Virus (NDV) and uninfected control group. There were four birds per Both groups raised on commercially available antibiotic free feed under a semi-controlled setup. binning step the identified 130 MAGs ≥50% completion, ≤10% contamination. data presented includes sequences in FASTA format,...

Understanding the interaction between environmental conditions, crop yields, and soil health is crucial for sustainable agriculture in a changing climate. Management practices to limit disease are balancing act. For example, potato production, dry conditions favour common scab (Streptomyces spp.) wet blackleg (Pectobacterium spp.). The exact mechanisms involved how these link changes microbiome unclear. Our objectives were test irrigation management bacterial pathogen load seed stocks...

Pit latrines are used by billions of people globally, often in developing countries where they provide a low-tech and low-cost sanitation method. However, health social problems can arise from lack emptying or maintenance these facilities. A better understanding the biological environmental parameters within pit could inform attempts to enhance material decomposition rates, therefore slow fill-up rate. In this study, we have performed spatial analysis 35 Tanzanian identify bacteria factors...

Abstract Farmers near towns and cities are using wide range of untreated wastewaters for crop irrigation in Pakistan due to severe freshwater shortage. The present study aimed treat different types wastewater including domestic, hospital, textile, pharmaceutical mixed indigenous bacterial isolates remove contaminants render these safer irrigation. 37 strains were isolated from the 5 samples collected sites Lahore, Pakistan. Under optimum growth conditions, D6, D7 P1 showed maximum...

Abstract Use of high-throughput sequencing is widespread in efforts to understand the microbial communities natural and engineered systems. Many built ecosystems, particular those used for wastewater treatment, have harnessed metabolic capacity complex effective removal recovery organic pollutants. Recent better precisely engineer such systems increasingly map structure function treatment microbiomes. An enormous amount data readily available on online repositories as National Center...