A5063196220- Gut microbiota and health
- Pancreatic and Hepatic Oncology Research
- Gastric Cancer Management and Outcomes
- Diet and metabolism studies
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Helicobacter pylori-related gastroenterology studies
- Nutritional Studies and Diet
- Nutrition and Health in Aging
- Inflammatory Bowel Disease
- Pancreatic function and diabetes
- Infant Nutrition and Health
- Liver Disease Diagnosis and Treatment
- Esophageal Cancer Research and Treatment
- Dietary Effects on Health
- Diabetes and associated disorders
- Microscopic Colitis
- Esophageal and GI Pathology
- Diet, Metabolism, and Disease
- Genomics and Phylogenetic Studies
- Hematopoietic Stem Cell Transplantation
- Microbial Community Ecology and Physiology
- Diabetes Management and Research
- Diabetic Foot Ulcer Assessment and Management
- Neutropenia and Cancer Infections
University of Amsterdam
2019-2024
Amsterdam University Medical Centers
2019-2024
Amsterdam UMC Location University of Amsterdam
2020-2022
Dana-Farber Cancer Institute
2019
Universitätsklinikum Erlangen
2019
Wageningen University & Research
2013-2018
Centre for Medical Systems Biology
2018
TiFN
2013-2016
York University
2015
City Of Hope National Medical Center
1992
ABSTRACT The composition and activity of the microbiota in human gastrointestinal tract are primarily shaped by nutrients derived from either food or host. Bacteria colonizing mucus layer have evolved to use mucin as a carbon energy source. One members mucosa-associated is Akkermansia muciniphila , which capable producing an extensive repertoire mucin-degrading enzymes. To further study substrate utilization abilities A. we constructed genome-scale metabolic model test amino acid auxotrophy,...
Next generation sequencing (NGS) technologies can be applied in complex microbial ecosystems for metatranscriptome analysis by employing direct cDNA sequencing, which is known as RNA (RNA-seq). RNA-seq generates large datasets of great complexity, the comprehensive interpretation requires a reliable bioinformatic pipeline. In this study, we focus on development such pipeline, validate using Illumina derived from small intestine microbiota two individuals with an ileostomy.The pipeline...
Donor fecal microbiota transplantation restores gut microbial diversity and reduces graft-versus-host disease in hematopoietic cell transplant recipients.
Akkermansia muciniphila colonizes the mucus layer of gastrointestinal tract, where organism can be exposed to oxygen that diffuses from epithelial cells. To understand how A. is able survive and grow at this oxic-anoxic interface, its tolerance response reduction capacities were studied. was found tolerant. On top this, under aerated conditions, showed significant growth rate yield increased compared those seen strict anaerobic conditions. Transcriptome analysis revealed an initial stress...
The gut microbiome is thought to play a role in depressive disorders, which makes it an attractive target for interventions. Both the and symptom levels vary substantially across ethnic groups. Thus, any intervention depression targeting requires understanding of microbiome-depression associations ethnicities. Analysing data from HELIUS cohort, we characterize microbiota its with symptoms 6 groups (Dutch, South-Asian Surinamese, African Ghanaian, Turkish, Moroccan; N = 3211), living same...
Abstract Aims Gut microbiota have been linked to blood lipid levels and cardiovascular diseases (CVDs). The composition abundance of gut trophic networks differ between ethnicities. We aim evaluate the relationship microbiotal CVD phenotypes. Methods results included cross-sectional data from 3860 individuals without history 6 ethnicities living in Amsterdam region participating prospective Healthy Life Urban Setting (HELIUS) study. Genetic variants were genotyped, faecal profiled,...
Lactobacillus rhamnosus is a diverse Gram-positive species with strains isolated from different ecological niches. Here, we report the genome sequence analysis of 40 L. and their genomic comparison, focus on variable genome. Genomic comparison discriminated conserved genes (core genome) regions plasticity involving frequent rearrangements horizontal transfer (variome). The core encompasses 2,164 genes, out 4,711 in total (the pan-genome). accessory dominated by encoding carbohydrate...
Cachexia is a multifactorial syndrome, associated with poor survival in patients cancer, and influenced by the gut microbiota. We investigated effects of fecal microbiota transplantation (FMT) on cachexia treatment response advanced gastroesophageal cancer.In double-blind randomized placebo-controlled trial performed Amsterdam University Medical Center, we assigned 24 cachectic metastatic HER2-negative cancer to either allogenic FMT (healthy obese donor) or autologous FMT, prior palliative...
Preclinical data suggest that treatment with either glucagon-like peptide (GLP)-1 receptor agonists or dipeptidyl peptidase (DPP)-4 inhibitors could change the intestinal microbiome and thereby contribute to their beneficial (cardio)metabolic effects. Therefore, our study aimed investigate effects of these agents on microbiota composition in adults type 2 diabetes (T2D). A total 51 T2D (mean ± SD: age 62.8 6.9 years, BMI 31.8 4.1 kg/m2, HbA1c 7.3 0.6%) treated metformin and/or sulphonylureas...
Abstract Background During the course of history, various important lifestyle changes have caused profound transitions gut microbiome. These include introduction agriculture and animal husbandry, a shift from nomadic to more sedentary lifestyle, recently increased levels urbanization transition towards Western lifestyle. The latter is linked with shifts in microbiome that reduced fermentative capability which are commonly associated diseases affluence. In this study, 5193 subjects included,...
Accumulating evidence shows that microbes with their theater of activity residing within the human intestinal tract (i.e., gut microbiome) influence host metabolism. Some strongest results come from recent fecal microbial transplant (FMT) studies relate changes in microbiota to various markers metabolism as well pathophysiology insulin resistance. Despite these developments, there is still a limited understanding multitude effects associated FMT on general physiology host, beyond microbiome...
Fecal microbiota transplantation (FMT) is under investigation for several indications, including ulcerative colitis (UC). The clinical success of FMT depends partly on the engraftment viable bacteria. Because vast majority human gut consists anaerobes, currently used aerobic processing protocols donor stool may diminish bacterial viability transplanted material. This study assessed effect four techniques (i.e., anaerobic and aerobic, both direct after temporary cool storage) viability. By...
Commercial pre-weaning diets are formulated to be highly digestible and nutrient-dense contain low levels of dietary fibre. In contrast, pigs in a natural setting manipulating fibre-rich plant material from young age. Moreover, fibre affects gastrointestinal tract (GIT) development health older pigs. We hypothesised that supplemental vegetal fibres accelerating GIT suckling piglets terms size functionality. From d 2 life, sow-suckled had access diet (CON), with fermentable long-chain...
Obesity and type 2 diabetes (T2D) are growing burdens for individuals the health-care system. Bariatric surgery is an efficient, but drastic treatment to reduce body weight, normalize glucose values, low-grade inflammation. The gut microbiome, which in part controlled by intestinal antibodies, such as IgA, involved development of both conditions. Knowledge effect bariatric on systemic antibody response limited. Here, we determined fecal microbiome 40 T2D non-diabetic (ND) obese that...
The intestinal gut microbiota is important for human metabolism and immunity can be influenced by many host factors. A recently emerged factor secreted microRNA (miRNA). Previously, it has been shown that miRNAs influence the growth of certain bacteria conversely, shifts in alter composition miRNAs. Here, we sought to further investigate interaction between use fecal transplantation (FMT). Subjects with metabolic syndrome received either an autologous (n = 4) or allogenic 14) FMT. Fecal...
Amino acids, metabolized by host cells as well commensal gut bacteria, have signaling effects on metabolism. Oral supplementation of the essential amino acid histidine has been shown to exert metabolic benefits. To investigate whether dietary aids glycemic control, we performed a case-controlled parallel clinical intervention study in participants with type 2 diabetes (T2D) and healthy controls. Participants received oral for seven weeks. After weeks supplementation, microbiome was depleted...
Introduction: Research has shown that those with ADHD have an increased risk for addiction disorders like alcoholism and substance abuse. What is less clear the mechanism(s) whereby gives rise to engagement in addictive behaviors, whether there are sex differences ADHD-addiction propensity. Both addictions also been associated personality traits such as impulsivity, reward seeking, anxiousness, negative affect. In this study, we tested a moderator-mediation model which predicted both...
High-protein diets are promoted for individuals with type 2 diabetes (T2D). However, effects of dietary protein interventions on (gut-derived) metabolites in T2D remains understudied. We therefore performed a multi-center, randomized-controlled, isocaloric intervention 151 participants following either 12-week high-protein (HP; 30Energy %, N = 78) vs. low-protein (LP; 10 Energy%, 73) diet. Primary objectives were glycemic control which determined via excursions, continuous glucose monitors...
Pakistan is a multi-ethnic society where there disparity between dietary habits, genetic composition, and environmental exposures. The microbial ecology of healthy Pakistani gut in the context anthropometric, sociodemographic, patterns holds interest by virtue it being one most populous countries, also Lower Middle Income Country (LMIC). 16S rRNA profiling microbiome normo-weight individuals from different regions residence performed with additional meta-data collected through filled...
Abstract Background The microbiome has been associated with chemotherapy and immune checkpoint inhibitor efficacy. How this pertains to resectable esophageal carcinoma is unknown. Our aim was identify microbial signatures in response neoadjuvant chemoradiotherapy or without an inhibitor. Methods From 2 prospectively collected cohorts (n = 172 total) treated alone 132) a combination of 40), fecal samples were available at baseline, during treatment, presurgery. Additionally, the...