We evaluated the effects of detergents based on sodium dodecyl sulfoxide (SDS) functional parameters collared peccary frozen-thawed sperm. Semen aliquots from ten individuals were diluted in a Tris-egg yolk-glycerol extender alone or with 0.5% Equex STM® paste SDS (at 0.1%, 0.3% (v/v) concentration). Samples fast frozen liquid nitrogen post-thaw evaluation motility, membrane functionality and integrity, mitochondrial activity, sperm binding ability thermal resistance. The treatments without...

The advancement of short-term storage methods for collared peccary semen targets its potential application in artificial insemination programmes and combination with cryopreservation techniques. objective this study was to evaluate the performance a transport container (Botutainer) preservation using commercial extenders (BTS, NUTRIXcell+ PRIMXcell Ultra) as well TRIS + egg yolk extender. Ten ejaculates obtained by electroejaculation were diluted stored at 5°C 72 h. cooled samples analysed...

The objective was to evaluate different permeating cryoprotectants vitrify testicular tissue biopsies from adult collared peccaries. Five pairs of testicles were dissected into fragments (9 mm³) that allocated non-vitrified (control) and vitrified groups using a solid-surface method following exposure (3.0 M dimethyl sulfoxide (DMSO), 3.0 ethylene glycol (EG) or 1.5 DMSO + EG). After warming, samples evaluated for histomorphology, ultrastructure, viability, proliferative capacity potential....

Studies on semen and sperm cells are critical to develop assisted reproductive technologies for the conservation of collared peccary. The objective study was compare effect different antibiotics bacterial load quality during short-term storage peccary semen. Fresh samples from 10 males were extended in Tris-egg yolk or Tris-Aloe vera supplemented with streptomycin-penicillin (SP; 1 mg/mL - 1000 IU/mL 2 2000 IU/mL) gentamicin (30 µg/mL 70 µg/mL) before at 5°C. Bacterial motility, membrane...

This study measured the effects of different freezing techniques and permeating cryoprotectants on preservation testicular tissues from adult red-rumped agoutis. Tissue biopsies (3.0 mm3) five individuals were allocated to experimental groups: control (non-cryopreserved); slow (SF), solid-surface vitrification (SSV), conventional (CV). Each method used dimethyl sulfoxide (DMSO), ethylene glycol (EG), or a DMSO + EG combination. Morphology, viability, mitochondrial activity, proliferative...

Methods for seminal plasma (SP) removal and the selection of collared peccary sperm fertilization were compared. The experiments evaluated following: (I) impact centrifugation SP before swim-up (II) a comparison different Percoll® gradient densities (PG 45–90% PG 35–70%). Non-selected served as control. Sperm quality was assessed based on motility patterns, morphology, membrane functional integrity, viability, reactive oxygen species (ROS), glutathione (GSH), DNA integrity. Subsequently,...

Abstract We evaluated the effects of supplementation with catalase (CAT) and superoxide dismutase (SOD) antioxidants, isolated or combined, on freezability collared peccary sperm. Semen aliquots from ten individuals were diluted in Tris‐yolk without presence antioxidants CAT (200 400 IU/mL) SOD (150 300 alone combination (CAT: 200 IU/mL + SOD: 150 IU/mL), then cryopreserved liquid nitrogen (−196°C). After 1 week, samples thawed for kinetic parameters, membrane functionality integrity,...

The objective was to investigate the effects of semen freezing extender supplementation with antibiotics on bacterial load samples, sperm functional and morphological metrics in collared peccary. Fresh ejaculates from 10 males were extended Tris-egg yolk-glycerol supplemented or not (control) gentamicin (70 μg/mL) streptomycin-penicillin (SP; 1 mg/mL-1000 IU/mL) cryopreserved liquid nitrogen. Bacterial load, motility patterns, morphology, membrane functionality integrity, mitochondrial...

The influence of environmental factors in a semiarid climate on characteristics fresh and frozen/thawed sperm collected from collared peccaries (Pecari tajacu) was assessed. Semen 11 male by electroejaculation during the peaks dry rainy periods while rainfall indices, air temperatures, relative humidity levels, wind speeds were measured. number, motility, morphology, osmotic response, membrane integrity ejaculates Samples then frozen liquid nitrogen, thawed, reassessed. index period (73.2...

Abstract In contributing to the conservation of wild rodents, aim this study was evaluate use distinct cryoprotectants, separately or in combination, for solid surface vitrification (SSV) red‐rumped agouti ovarian tissue. Ovarian cortex from nine females recovered and fragmented. Fresh fragments (control) were used analyse pre‐antral follicle (PF) morphology using a histologic procedure, viability Trypan blue test, cell proliferation by counting argyrophilic nucleolar organizing regions...

Abstract We studied the sperm membrane functionality through epididymal transit by comparing different hypoosmotic solutions and verifying possible associations among osmotic response functional parameters of in red‐rumped agouti ( Dasyprocta leporina ). For this purpose, from six sexually mature male agoutis were collected via flotation. Then, analyses swelling test using (0, 50 200 mOsm/L) regions epididymis (caput, corpus cauda) performed. There was an increase p < .05) values for...

Summary We compare the efficiency of mechanical or enzymatic methods, and their combination, for isolation ovarian preantral follicles (PFs) from collared peccaries. The ovaries six females were subjected to different methods investigated here. For method, ovary fragments exposed collagenase type IV in TCM-HEPES medium; procedure was based on cortex dissociation by using a scalpel blade. residual solution obtained after procedure. number isolated PFs quantified classified as primordial,...

1. The objectives of this study were to establish the use fluorophores Hoechst 33342 and propidium iodide for evaluation sperm plasma membrane integrity identify an adequate hypoosmotic solution functionality in quails.2. Sperm samples collected from vas deferens nine quails. After initial evaluation, subjected a flash-frozen assay. Three treatments with following proportions fresh flash freezing prepared as follows: 100:0 (T100), 50:50 (T50), 0:100 (T0). swelling test used distilled water...

Abstract The use of assisted reproductive techniques, such as chilled semen, contributes to the maintenance and genetic improvement canine breeding. INRA‐96 extender is a commercially available, chemically defined medium that was initially developed for preservation equine semen exhibits potential in species. This research aims evaluate an alternative short‐term terms sperm quality parameters motility kinetic parameters, integrity functionality plasma membrane fresh chilled–rewarmed samples,...

The objectives of the study were to (1) describe kinematic parameters spermatozoa (2) compare methods evaluating sperm viability (3) validate assays functionality and integrity membrane (4) evaluate possible changes between from epididymis vas deferens greater rhea. Semen samples recovered 7 adult individuals. Sperm motility was characterized by adjusting set-up for Computer-assisted semen analysis (CASA) that new species. For evaluation, smears bromophenol blue eosin-nigrosine dyes used....