We report a novel strategy of simultaneous in situ extraction and fabrication surface-enhanced Raman scattering substrate (IE-SERS) to perform selective reliable on-site determination thiram residue soil, fruits, vegetables. In this protocol, the on complex surfaces can facilely diffuse into solvent (dichloromethane (DCM)) specifically bind gold nanoparticles (AuNPs), affording SERS through embedding thiram-trapped AuNPs cellulose p-toluenesulfonates (CTSAs) film evaporation DCM. signals...

Real-time in situ monitoring of miRNAs living cells is often appealed to signal amplifiers tackle their low abundance challenges. However, the poor kinetics and potential interferences from complex intracellular environment hamper its widespread applications vivo. Herein, we report a framework nucleic acid (FNA)-based nonenzymatic spatial-confinement amplifier for rapid reliable miRNA imaging. The consists localized catalytic hairpin assembly (L-CHA) reactor encapsulated inner cavity an FNA...

Identification of protein profiling on plasma exosomes by SERS can be a promising strategy for early cancer diagnosis. However, it is still challenging to detect multiple exosomal proteins simultaneously since the Raman signals detected conventional colloidal nanocrystals or two-dimensional substrates are incomplete and complex. Herein, we develop novel three-dimensional (3D) surround-enhancing platform, named 3D se-SERS, multiplex detection proteins. In this covered with "hotspots"...

Nitrogen-doped coral-like porous carbon embedded with small alloy nanoparticles was synthesized by a direct surfactant co-assembly approach and demonstrated excellent nitrate electrocatalysis ability.

Colorimetric analytical strategies exhibit great promise in developing on-site detection methods for antibiotics, while substantial recent research efforts remain problematic due to dissatisfactory sensitivity. Taking this into account, we develop a novel colorimetric sensor in-field of antibiotics by using aptamer (Apt)-capped and horseradish peroxidise (HRP)-embedded zeolitic metal azolate framework-7 (MAF-7) (Apt/HRP@MAF-7) as target recognition signal transduction, respectively. With the...

In situ rapid detection of contaminants in environmental water is crucial for protecting the ecological environment and human health; however, it always hindered by complexity sample matrices, trace content, unknown species. Herein, we demonstrate a deep learning-based multicapturer surface-enhanced Raman scattering (SERS) platform on plasmonic nanocube metasurfaces multiplex determination organophosphorus pesticides (OPPs) residues. Poly(vinylpyrrolidone), 4-mercaptobenzoic acid, l-cysteine...

A novel facile method for on-site determination of sulphite in wine using a gas-diffusion microfluidic paper-based analytical device (μPAD) combined with surface-enhanced Raman spectroscopy (SERS) was explored. The inexpensive and disposable μPAD realized by sandwiching the ZnO-paper disc integrating separation gas preconcentration on platform. Through extensive condition optimization, SERS signal at shift 620 cm(-1) concentration SO2 showed good linearity range 5-300 μg mL(-1). linear...

A series of LMOFs with AIE and ESIPT properties, tunable emissions large Stokes shifts has been facilely synthesized by binding AIEgens nanoscale ZIF-8 to realize sensitive sensing copper(<sc>ii</sc>) visual fluorescence detection glucose.

Rapid component separation and reliable surface-enhanced Raman scattering (SERS) detection of organochlorine pesticide (OCP) residues in real water samples remain major challenges because sample complexity, trace content, low molecular affinity for a metal surface. Here, we report novel strategy simultaneous situ extraction fabrication plasmonic colloidal gold superparticles (AuSPs) to perform rapid SERS OCPs environmental water. In this protocol, multiple components complex were facilely...

Timely and accurate diagnosis of childhood epilepsy identification its etiology are crucial for early interventional treatment, yet still, effective detection methods lacking. Blood analysis is a promising strategy disease diagnosis. However, due to the complex composition lack definite diagnostic markers in serum, comprehensively profiling serum molecular signals accurately reveal information still challenging. Herein, we developed novel magnetically driven SERS platform, which utilized...

Surface-enhanced Raman-scattering-based (SERS-based) biosensing in biological fluids is constrained by nonspecific macromolecule adsorptions and disposable property of the SERS substrate. Here, novel multi-Au-nanoparticle-embedded mesoporous silica microspheres (AuNPs/mSiO2) were prepared using a one-pot method, which served as reliable substrates for enhancement associated with salient features self-filtering ability reusability. The fabrication physical characterization AuNPs/mSiO2...

Due to low optical activity and structural simplicity, synchronous chiral discrimination identification of aliphatic amino acids (AAs) are still challenging yet demanding. Herein, we developed a novel surface-enhanced Raman spectroscopy (SERS)-based discrimination-sensing platform for AAs, in which l- d-enantiomers able discriminately bind with quinine generate distinct differences the SERS vibrational modes. Meanwhile, plasmonic sub-nanometer gaps supported by rigid enable maximization...

A new strategy by converging ultrafiltration high-performance liquid chromatography with ultraviolet and mass spectrometry pH-zone-refining counter-current was developed for the rapid screening separation of potential acetylcholinesterase inhibitors from crude alkaloidals extract Zanthoxylum nitidum. An optimized two-phase solvent system composed chloroform/methanol/water (4:3:3, v/v) used in this study. And, optimal system, 45 mM hydrochloric acid added to aqueous stationary phase as...

Developing a facile and sensitive sensing platform is of importance for point-of-care testing (POCT). Herein, portable POCT based on "dual gates" aminated magnetic mesoporous silica nanocomposites (AMMS) bearing polydopamine (PDA)-aptamer (Apt) two-tier shells, as novel nanodevice, designed target detection through target-triggered glucose (GO) release from AMMS with personal glucometer (PGM) readout. In the absence target, GO can be firmly captured in pores by gates", which would decrease...

A digital fluorescence detector (DFD), a handheld detection device, can convert the signal of samples into corresponding fluorescer concentration. Herein, by adopting DFD as readout, novel intelligent platform was developed based on ratiometric paper-based device (RPD) for multiple aminoglycoside detection. There are five layers and four parallel channels contained in designed RPD, functioning reagent storage, fluidic path control processing, respectively. The rationale this design lies fact...

Noble metal nanoparticles (NPs) have enabled surface-enhanced Raman scattering (SERS) for in situ monitoring of NPs-catalyzed reactions. However, it still remains a great challenge to ensure that analytes without plasmonic surface-affinity groups (such as thiol and amino groups) can be located into hotspots detected by SERS. Here, we report novel sacrificial template method the fabrication "pomegranate-like" nanoreactors (PPNs), which high-density embedded AuNPs simultaneously generated SERS...