A5041608970- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- Toxin Mechanisms and Immunotoxins
- Growth Hormone and Insulin-like Growth Factors
- HIV/AIDS Research and Interventions
- Transgenic Plants and Applications
- RNA Interference and Gene Delivery
- Hematopoietic Stem Cell Transplantation
- Endoplasmic Reticulum Stress and Disease
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Cell death mechanisms and regulation
- Biochemical and Molecular Research
- Cancer therapeutics and mechanisms
- Nuclear Receptors and Signaling
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Drug Transport and Resistance Mechanisms
- Erythropoietin and Anemia Treatment
- PI3K/AKT/mTOR signaling in cancer
- Erythrocyte Function and Pathophysiology
- Wnt/β-catenin signaling in development and cancer
- Cancer Mechanisms and Therapy
- Mesenchymal stem cell research
- Plant tissue culture and regeneration
Rutgers, The State University of New Jersey
1998-2020
Johnson University
1998-2002
Rutgers Sexual and Reproductive Health and Rights
1996
ABSTRACT The diploid nature of human immunodeficiency virus type 1 (HIV-1) indicates that recombination serves a central function in replication and evolution. Previously, while examining the obligatory primer strand transfers during reverse transcription, high rate was observed at ends viral genome within long terminal repeats, prompting following question: does occur throughout genome? To address this question, two vectors based upon different strains HIV-1 were utilized. comprised...
Previously, we reported that human immunodeficiency virus type 1 (HIV-1) recombines approximately two to three times per genome replication cycle, an extremely high rate of recombination given the relatively small size HIV-1. However, a hot spot involving sequence nonretroviral origin was identified in vector system utilized, raising possibility this skewed recombination, and observed overestimation. To address issue, HIV-1-derived used examine between autologous HIV-1 sequences after...
The diploid nature of human immunodeficiency virus type 1 (HIV-1) suggests that recombination serves a central function in replication and evolution. A system was developed to examine HIV-1 strand transfers, including the obligatory DNA primer transfers as well recombinational crossovers during reverse transcription. Sequence heterogeneity between different strains exploited for examining transfer events. Both intra- intermolecular were observed at similar frequencies minus-strand synthesis,...
In nontransformed bovine mammary epithelial cells, the intrinsic apoptosis inducer anisomycin (ANS) induces IGFBP-3 expression and nuclear localization knockdown of attenuates ANS-induced apoptosis. Others have shown in prostate cancer cells that exogenous by facilitating export orphan receptor Nur77 its binding partner, retinoid X receptor-α (RXRα). The goal present work was to determine whether endogenous plays a role transport and/or RXRα cells. Knockdown with siRNA decreased cleavage...
Ricin is a highly toxic type II ribosome-inactivating protein that has potential as biochemical weapon and the component of immunotoxins. The unfolded response (UPR) survival helps cells to recover from endoplasmic reticulum (ER) stress. Failure ER stress leads apoptosis. In yeast, ricin-A-chain (RTA), enzymatic ricin, inhibits UPR. Our goals were determine if RTA UPR in two epithelial cell lines this affects cytotoxicity. alone did not induce However, inhibited both phosphorylation...
Mammary epithelial cell (MEC) number is an important determinant of milk production in lactating dairy cows. IGF-I increases IGF binding protein-3 (IGFBP-3) these cells, which plays a role its ability to enhance proliferation. In the present study, we show that apoptotic factor anisomycin (ANS) also IGFBP-3 mRNA and protein dose- concentration-dependent manner mirrors activation caspase-3 -7, with significant both caspase observed by 3 h. Knock-down small interfering (si) RNA attenuated ANS...
IGF-binding protein (IGFBP)-3 is a multifunctional that can exert IGF-independent effects on apoptosis. Anisomycin (ANS) potent inducer of IGFBP-3 production in bovine mammary epithelial cells (MECs), and knockdown attenuates ANS-induced present the nucleus conditioned media response to ANS. The goal this study was determine whether ribotoxic stress induced by ANS or second ribotoxin, deoxynivalenol (DON), specifically regulates transport pathway which it traffics. In ribotoxin-treated...
Fetal alcohol exposure (FAE) increases the risk of mammary tumorigenesis in adult offspring; however, underlying mechanism remains unknown. This study tested hypothesis that FAE shifts epithelial cell (MEC) composition toward one promotes tumorigenesis.