A5018244216- Skin and Cellular Biology Research
- Hair Growth and Disorders
- Genetic and phenotypic traits in livestock
- Meat and Animal Product Quality
- RNA regulation and disease
- Animal Genetics and Reproduction
- Adipose Tissue and Metabolism
- Muscle Physiology and Disorders
- Animal Behavior and Welfare Studies
- Animal Disease Management and Epidemiology
- Calpain Protease Function and Regulation
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Microbial infections and disease research
- RNA Research and Splicing
- Wnt/β-catenin signaling in development and cancer
- Pharmacological Effects and Assays
- Silk-based biomaterials and applications
- Peroxisome Proliferator-Activated Receptors
- Lipid metabolism and biosynthesis
- Insect and Pesticide Research
- Streptococcal Infections and Treatments
- Immune Response and Inflammation
- melanin and skin pigmentation
- Dyeing and Modifying Textile Fibers
Lincoln University
2016-2025
Gansu Agricultural University
2010-2024
Xinjiang Academy of Agricultural and Reclamation Science
2017-2018
Lincoln University - Pennsylvania
2000-2014
Genomic selection (GS) has changed cattle breeding, but its use so far been in selecting superior bulls for breeding. However, farm-level impact, particularly on female selection, remains less explored. This study aimed to investigate the impact of implementing GS identify cows and using artificial mating those with sex-selected semen a New Zealand Holstein-Friesian (HF) dairy herd (n = 1800 cows). Heifers 2061) born over four consecutive years between 2021 2024 were genotyped their genomic...
Myostatin is a regulator of myogenesis and has been implicated in the regulation adiposity controlling structure function tendons. Polymerase Chain Reaction Single-Stranded Conformational Polymorphism (PCR-SSCP) analysis intron-1 was used to identify five variants (designated A-E) myostatin gene (MSTN). The effect this genetic variation on growth carcass traits investigated 517 Romney male lambs from 17 sire-lines, born South Island New Zealand farm. General linear mixed models revealed that...
Keratin (K) is a major protein component of hair and involved in growth development. In this study, we analysed the expression, localization, polymorphism K84 gene (KRT84) Gansu Alpine Fine-wool sheep using immunofluorescence, RT-qPCR, PARMS (penta-primer amplification refractory mutation system). Haplotypes KRT84 were also constructed their relationship with wool traits analysed. It was revealed that highly expressed follicles, including inner root sheath, outer medulla at all six lamb ages...
Sheep’s wool is known to have unique biological, physical and chemical properties. The fibre primarily consists of proteins, but these amino acid sequence variation, at the phenotypic level varies considerably. This can affect its utility value. Unravelling genetic factors that underpin protein variability crucial if we are contemplate improving quality. Accordingly, this study investigates high glycine tyrosine content keratin-associated 19-5 gene (KRTAP19-5) in sheep. PCR-single strand...
Insulin-like growth factor 1 (IGF-1) has been identified as an important in many biological systems.[1] It shares considerable structural homology with insulin and exerts insulin-like effects on food intake glucose metabolism. Recently it suggested to play a role regulating cellular proliferation migration during the development of hair follicles. [2,3] To exert its effects, IGF-1 is required activate cells by binding specific cell-surface receptors. The type I IGF receptor (IGF-1R) only...
Summary High glycine–tyrosine keratin‐associated proteins ( HGT ‐ KAP s) are predominantly present in the orthocortex of wool fibres. They vary abundance different wools and have been implicated regulating fibre properties, but little is known about functional roles these matrix. In this study, we used polymerase chain reaction – single‐strand conformational polymorphism PCR SSCP ) analysis to screen for variation a gene encoding ovine 6‐1 protein. We identified three variants A , B C )....
Keratin-associated proteins (KAPs) are structural components of wool and hair fibers. To date, eight high glycine/tyrosine KAP (HGT-KAP) families have been identified in humans, but only three sheep. In this study, the putative ovine homolog human KAP22-1 gene (KRTAP22-1) was amplified using primers designed based on a KRTAP22-1 sequence. Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) used to screen for variation 390 Merino × Southdown-cross lambs 75 New...
The biochemistry of intermuscular variation in tenderness is not fully understood. To investigate the role calpains this process we performed two experiments using bovine and ovine species. In experiment, distinct muscles, longissimus thoracis et lumborum (LT) psoas major (PM), were used. four LT, PM, semimembranosus (SM), semitendinosus (ST), Muscles sampled at death for determination steady-state mRNA level calpastatin activities calpain 1, 2, calpastatin. also to determine temporal...
Summary Variation in the nucleotide sequence of B2 high‐sulfur protein genes has not been reported previously. This paper reports 15 substitutions each for B2A and B2C proteins a length polymorphism gene which translates to insertion/deletion one 30‐nucleotide repeat sequence. Evidence is presented conversion occurring within multigene family. These DNA polymorphisms may account some microheterogeneity observed also be useful genetic markers loci future use analysing wool fibre characteristics.
The keratin-associated proteins (KAPs) are structural components of cashmere fibres. gene encoding the high-sulphur (HS)-KAP24-1 (KRTAP24-1) has been identified in humans and sheep, but it not described goats. In this study, we report identification caprine KRTAP24-1, describe variation gene, investigate effect on traits. A search for sequences orthologous to ovine goat genome revealed a 774 bp open reading frame chromosome 1, which could encode an HS-KAP. Based sequence comparison with...
Keratins are the main structural protein components of wool fibres, and variation in them their genes (KRTs) is thought to influence structure characteristics. The PCR–single strand conformation polymorphism technique has been used previously investigate genetic selected coding intron regions type II sheep keratin gene KRT81, but no was identified. In this study, we same explore 5′ untranslated region KRT81 detected three sequence variants (A, B C) that contain four single nucleotide...
Journal Article Rapid communication: PCR-RFLP for MspI and NcoI in the ovine calpastatin gene Get access B. R. Palmer, Palmer 2Molecular Biotechnology, Animal Veterinary Sciences, Lincoln University, Canterbury, New Zealand Search other works by this author on: Oxford Academic PubMed Google Scholar N. Roberts, Roberts J.G.H. Hickford, Hickford Bickerstaffe of Science, Volume 76, Issue 5, May 1998, Pages 1499–1500, https://doi.org/10.2527/1998.7651499x Published: 01 1998 history Received: 15...
Variation in the ovine DQA2 gene was investigated approximately 2,000 sheep from six breeds. Fragments of DNA containing exon 2 were amplified using PCR. Single-strand conformational polymorphism analysis and sequence employed to detect genetic variation. Twenty-three nucleic acid sequences, encoding 22 amino identified. This increases number alleles identified 10 23. In some cases, three or four unique sequences isolated individual sheep, suggesting that these may represent two loci....