A5004628461- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Photoreceptor and optogenetics research
- Retinoids in leukemia and cellular processes
- Cancer, Hypoxia, and Metabolism
- Glaucoma and retinal disorders
- interferon and immune responses
- Circadian rhythm and melatonin
- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- Retinopathy of Prematurity Studies
- Immunotherapy and Immune Responses
- High Altitude and Hypoxia
- Cholesterol and Lipid Metabolism
- Fungal and yeast genetics research
- Antioxidant Activity and Oxidative Stress
- Retinal Imaging and Analysis
- Virus-based gene therapy research
- Connexins and lens biology
- Hepatitis B Virus Studies
- Retinal and Macular Surgery
- Cytokine Signaling Pathways and Interactions
University of Zurich
2016-2025
University Hospital of Zurich
2012-2025
Swiss Integrative Center for Human Health
2012-2022
University Hospital Frankfurt
2020-2021
Goethe University Frankfurt
2020-2021
Sartorius (Germany)
2021
Immunologie-Zentrum Zürich
2017
Comprehensive Cancer Center Vienna
2017
Medical University of Vienna
2010-2017
ETH Zurich
2014
10-20% of individuals over the age 65 suffer from age-related macular degeneration (AMD), leading cause severe visual impairment in humans living developed countries. The pathogenesis this complex disease is poorly understood, and no efficient therapy or prevention exists to date. A precondition for AMD appears be accumulation pigment lipofuscin lysosomes retinal epithelial (RPE) cells. In AMD, these cells seem die by apoptosis with subsequent death photoreceptor cells, light may accelerate...
Excessive light can cause retinal degeneration and may be an environmental cofactor accelerating dystrophies age-related diseases. In rodent models, the damage susceptibility (LDS) of retina is determined genetically. two mouse strains, with different degrees LDS, a Leu450Met variation in pigment epithelial protein RPE65 was shown recently to cosegregate low LDS. Because rhodopsin-mediated, essential for regeneration rhodopsin visual cycle, we analyzed this regarding metabolism LDS four...
Optical coherence tomography (OCT) is a novel method of retinal in vivo imaging. In this study, we assessed the potential OCT to yield histology-analogue sections mouse models degeneration.We achieved adapt commercial 3(rd) generation system obtain and quantify high-resolution morphological retina which so far required vitro histology. histology were compared with developmental defects, light damage, inherited degenerations. conditional knockout mice deficient retinoblastoma protein Rb,...
Purpose.: Spectral domain optical coherence tomography (SD-OCT) allows cross-sectional visualization of retinal structures in vivo. Here, the authors report efficacy a commercially available SD-OCT device to study mouse models degeneration. Methods.: C57BL/6 and BALB/c wild-type mice three different hereditary degeneration (Rho −/−, rd1, RPE65 −/−) were investigated using confocal scanning laser ophthalmoscopy (cSLO) for en face imaging structures. Histology was performed correlate...
Reactive microglia are commonly seen in retinal degenerative diseases, and neurotoxic responses can contribute to photoreceptor cell death. We others have previously shown that translocator protein (18 kDa) (TSPO) is highly induced degenerations the selective TSPO ligand XBD173 (AC-5216, emapunil) exerts strong anti-inflammatory effects on vitro ex vivo. Here, we investigated whether targeting with has immuno-modulatory neuroprotective functions two mouse models of acute degeneration using...
Age-related macular degeneration (AMD) is a progressive disease of the retinal pigment epithelium (RPE) and retina leading to loss central vision. Polymorphisms in genes involved lipid metabolism, including ATP-binding cassette transporter A1 ( ABCA1 ), have been associated with AMD risk. However, significance handling for pathogenesis remains elusive. Here, we study contribution efflux RPE by generating mouse model lacking its partner ABCG1 specifically this layer. Mutant mice show...
To characterize developmental defects and the time course of Norrie disease in retinal hyaloid vasculature during development to identify underlying molecular angiogenic pathways that may be affected disease, exudative vitreoretinopathy, retinopathy prematurity, Coats' disease.Norrie pseudoglioma homologue (Ndph)-knockout mice were studied at early postnatal (p) stages (p5, p10, p15, p21). Histologic techniques, quantitative RT-PCR, ELISA, Western blot analyses provided data, scanning laser...
White light (5 klux for 2 hr) induces apoptosis of rod photoreceptors in wild-type mice ( c-fos +/+ ) within 24 hr, whereas rods knock-out −/− are protected (Hafezi et al., 1997b). The range this protection was tested by analyzing retinas and up to 10 d after exposure threefold increased intensities (15 hr). In mice, were unaffected, they destroyed mice. After exposure, mitochondrial damage observed exclusively Electroretinograms recorded 48 hr revealed a decrease all components but...
Survival and death of photoreceptors in degenerative diseases the retina is controlled by a multitude genes endogenous factors. Some may be involved process itself whereas others part an defense system. We show two models retinal degeneration that photoreceptor strongly induces expression leukemia inhibitory factor (LIF) subset Muller glia cells inner nuclear layer retina. LIF essential to induce extensive intraretinal signaling system which includes characterized upregulation Edn2, STAT3,...
Retinal degeneration is a major cause of severe visual impairment or blindness. Understanding the underlying molecular mechanisms prerequisite to develop therapeutic approaches for human patients. We show in three mouse models that induced and inherited retinal induces LIF CLC as members interleukin (IL)-6 family proteins, activates proteins Jak-STAT signaling pathway, up-regulates suppressors cytokine negative feedback loop. Inhibition Jak2 leads protection photoreceptors model but not...
The number of patients age >65 years with malignant gliomas is increasing. Prognosis these worse compared younger patients. To determine biological differences among different groups and help to explain the survival heterogeneity seen in NOA-08 trial, prevalence impact recently established biomarkers for outcome were characterized elderly Prevalences mutations isocitrate dehydrogenase 1 (IDH1) histone H3.3 (H3F3A), glioma cytosine–phosphate–guanine island methylator phenotype (G-CIMP),...
Abstract In lower vertebrates, such as fish, Müller glia plays an essential role in the restoration of visual function after retinal degeneration by transdifferentiating into photoreceptors and other neurons. During this process, cells re‐enter cell cycle, proliferate, migrate from inner nuclear layer (INL) to photoreceptor where they express photoreceptor‐specific markers. This process transdifferentiation is absent mammals, loss leads permanent vision deficits. The mechanisms underlying...