A5069832311- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Chronic Myeloid Leukemia Treatments
- Protein Degradation and Inhibitors
- Immunodeficiency and Autoimmune Disorders
- CAR-T cell therapy research
- Chemical Synthesis and Analysis
- Mast cells and histamine
- Osteomyelitis and Bone Disorders Research
- Trace Elements in Health
- Inflammasome and immune disorders
- Plant tissue culture and regeneration
- Chronic Lymphocytic Leukemia Research
- HIV Research and Treatment
- Click Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- Anodic Oxide Films and Nanostructures
- Phagocytosis and Immune Regulation
- Histone Deacetylase Inhibitors Research
- Pancreatic function and diabetes
- Ubiquitin and proteasome pathways
- Cell Adhesion Molecules Research
- Erythrocyte Function and Pathophysiology
Fox Chase Cancer Center
2015-2019
University of Colorado Denver
2018
Bristol-Myers Squibb (United States)
2018
Goethe University Frankfurt
2009-2015
St. Jude Children's Research Hospital
2014
Center for Clinical Research (United States)
2007-2009
University Hospital Münster
2007
Mouse B cell precursors from fetal liver and adult bone marrow (BM) generate distinctive progeny when transplanted into immunodeficient recipients, supporting a two-pathway model for lymphopoiesis, “B-1” “B-2.” Recently, Lin28b was shown to be important the switch between pathways; however, neither mechanism of action nor importance antigen receptor (BCR) signaling in this process addressed. Here, we report key advances our understanding regulation B-1/B-2 development. First, modulation...
Deep sequencing and single-chain variable fragment (scFv) yeast display methods are becoming more popular for discovery of therapeutic antibody candidates in mouse B cell repertoires. In this study, we compare a deep scFv method that retains native heavy light chain pairing with related randomly pairs chain. We performed the studies humanized mouse, using interleukin 21 receptor (IL-21R) as test immunogen. identified 44 high-affinity binder 100 random method. 30% natively paired binders were...
The Lin28b+Let7– axis in fetal/neonatal development plays a role promoting CD5+ B1a cell generation as B-1 B developmental outcome. Here we identify the Let7 target, Arid3a, crucial molecular effector of program. Arid3a expression is increased at pro-B stage and markedly pre-B immature stages liver relative to that Lin28b–Let7+ adult bone marrow (BM) B-2 development. Analysis B-lineage restricted Lin28b transgenic (Tg) mice, knockout Tg confirmed allows without requiring surrogate light...
FLT3 and c-KIT are crucial regulators of hematopoietic stem progenitor cells. We investigated the role STS1 STS2 on phosphorylation, activity, function in normal stress-induced hematopoiesis. STS1/STS2-deficient mice show a profound expansion multipotent lymphoid primed cells with elevated colony-forming capacity. Although long-term not increased numbers, lack significantly promotes repopulation demonstrating pivotal STS1/STS2 regulating cell fitness. Biochemical analysis identified as...
Abstract Prostate cancer is the most common in American men and second leading cause of death western countries. Most metastatic patients develop castration resistant prostate cancer, which despite advances immunotherapy, limited treatment options represents a critical unmet need. Bi-specific T cell engagers (TCEs) targeting antigens represent modality that has demonstrated preliminary clinical activity. Clinically validated are highly expressed cancers include Prostate-Specific Membrane...
Abstract Resulting from intracellular translation of nanoparticle delivered mRNA, mRNA therapeutics offers an unparalleled opportunity to direct the expression proteins specific subcellular compartments. Many cytokines, especially immunomodulatory Tumor Necrosis Factor (TNF) superfamily members, exist as both membrane and soluble proteins. Trimeric members TNF are known be susceptible protease cleavage resulting in their release membrane. Membrane versions immunomodulators may have different...
2537 Background: The approval of anti-CD20 monoclonal antibody Rituximab more than 20 years ago opened a new class highly effective immunotherapies for the treatment B cell lymphomas. However, use treatments that target only single tumor antigen, such as CD20 or CD19, can apply selective pressure to tumors results in loss down-regulation antigen expression patients. To address this issue, Nutcracker Therapeutics has developed NTX-472, nanoparticle formulated mRNA-based therapeutic candidate...
Plant based vaccines provide an instructive opportunity for immunologists. We have developed a plantbased oral vaccine against verocytotoxin–producing E. coli (VTEC) in piglets (Rossi et. al 2014). engineered two independent lines of Nicotiana tabacum plants the seed-specific expression VTEC antigens, represented by major subunit FedA F18 adhesive fimbriae and B-subunit VT2e toxin respectively al. 2013). Edible particular are interest as they able to stimulate mucosal immune system produce...
<h3>Background</h3> A transformative approach to medicine involves expressing complex and therapeutically relevant biologics utilizing patients' own body tissues via mRNA administration. Here, we engineered molecules targeting CD47, as multivalent SIRPa-Fc fusion proteins or bi-specifics both CD47 CCR4 molecules. is a widely expressed transmembrane glycoprotein that sends 'do not eat me' signals macrophages by binding signal regulatory protein alpha (SIRPa). With more than 50 ongoing...