A5032390025- Lipid Membrane Structure and Behavior
- Erythrocyte Function and Pathophysiology
- Cellular transport and secretion
- Alkaline Phosphatase Research Studies
- Force Microscopy Techniques and Applications
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- Influenza Virus Research Studies
- Neurological diseases and metabolism
- Monoclonal and Polyclonal Antibodies Research
- Prion Diseases and Protein Misfolding
- Lysosomal Storage Disorders Research
- Rabies epidemiology and control
- Protein Structure and Dynamics
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Trace Elements in Health
- Virology and Viral Diseases
- HIV Research and Treatment
- Respiratory viral infections research
- Toxin Mechanisms and Immunotoxins
- Mechanical and Optical Resonators
- Field-Flow Fractionation Techniques
- Parathyroid Disorders and Treatments
Sanofi (France)
2009-2023
Institut Mérieux (France)
2007-2020
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2006
Université Claude Bernard Lyon 1
1997-2006
Centre National de la Recherche Scientifique
1997-2005
Laboratoire de physique de la matière condensée
2004-2005
École Centrale de Lyon
2002
Université de Bordeaux
2002
A three-step detergent-mediated reconstitution has been applied to the incorporation of a glycosyl-phosphatidylinositol-protein into liposomes. The protein studied was alkaline phosphatase from bovine intestine. Liposomes prepared by dialysis were treated with various amounts two detergents, either n-octyl beta-D-glucoside or Triton X-100. At different steps solubilization process, added and detergent removed hydrophobic resins. most efficient reconstitutions obtained an octyl glucoside...
Transgenic mice expressing the prion protein (PrP) of species affected by transmissible spongiform encephalopathies (TSEs) have recently been produced to facilitate experimental transmission these diseases comparison with wild-type mice. However, whilst largely described for discrimination different TSE strains, including differentiation agents involved in bovine encephalopathy (BSE) and scrapie, this has only poorly transgenic Here, two ovine mouse lines (TgOvPrP4 TgOvPrP59), PrP (A136 R154...
Mammalian alkaline phosphatases (AP) are glycosylphosphatidylinositol (GPI) anchored proteins that localized on the outer layer of plasma membrane. The GPI anchors covalently attached to C-termini and consist a glycan chain bonded phosphatidylinositol with two acyl chains into membrane bilayer. Force spectroscopy, based atomic force microscope (AFM) technology, was used determine adhesion phosphatase in absence presence anchors. increase markedly frequency (i.e., protein affinity for...
The addition of aluminum-based adjuvants in vaccines enhances the immune response to antigens. strength antigen adsorption on adjuvant gels is known modulate vaccine efficacy. However, a detailed understanding mechanisms interaction between aluminum and antigens still missing. Herein, new analytical approach based dynamic nuclear polarization (DNP) enhanced NMR spectroscopy under magic angle spinning (MAS) implemented provide molecular description antigen-adjuvant interface. This...
Membrane microdomains are involved in numerous cellular processes such as membrane transport and signal transduction. Among these functionally microdomains, rafts enriched sphingolipids cholesterol their existence is actually mainly explained by a fluid-ordered lipid lateral phase separation. They also concentrate proteins like with C-terminal glycosylphosphatidylinositol (GPI) modification that serves an anchor to the plasma membrane. In order better understand how GPI partition into...
The monolayer organization of intestinal alkaline phosphatase (AP), a glycosylphosphatidyl inositol (GPI) anchored dimeric protein (AP-GPI), was monitored using polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS) and Brewster angle microscopy (BAM). behavior the reversible during compression−expansion cycles as shown by BAM. At high surface pressure, we observed formation condensed domains. Upon decompression, clusters remained stable over period several tenths...
To study the pathogenesis of bovine spongiform encephalopathy infection in small ruminants, two Lacaune sheep with AA136RR154QQ171 and one AA136RR154RR171 genotype for prion protein, were inoculated a brain homogenate from French cattle BSE case by peripheral routes. Sheep ARQ/ARQ are considered as susceptible to diseases contrary those ARR/ARR genotype. The accumulation disease-associated protein (PrP(d)) was analysed biochemical immunohistochemical methods. No PrP(d) detected samples 2...
Differential Scanning Calorimetry (DSC) has been used in the past to study thermal unfolding of many different viruses. Here we present first DSC analysis rabies virus. We show that non-inactivated, purified virus unfolds cooperatively two events centered at approximately 62 and 73 °C. Beta-propiolactone (BPL) treatment does not alter significantly viral behavior, indicating inactivation protein structure significantly. The event was absent bromelain treated samples, causing an elimination...
Fusion of the influenza A H1N1 virus envelope with endosomal membrane at low pH allows intracellular delivery viral genome and plays an essential role in infection process. Low induces irreversible modification envelope, which has so far resisted 3D structural analysis, partly due to pleiomorphy. This study showed that atomic force microscopy (AFM) physiological buffer could be used image details both neutral after a low-pH treatment. At intermediate magnification, AFM control virions...
An inactivated form of pertussis toxin (PTX) is the primary component currently available acellular vaccines against Bordetella , causative agent whooping cough. The PTX analyzed here purified at industrial scale and subsequently using glutaraldehyde. influence this treatment on antibody recognition crucial importance in study. Surface plasmon resonance (SPR) experiments its (toxoid) with 10 different monoclonal antibodies were conducted. was found to recognize an average affinity 1.34 ±...
A new method based on combined atomic force microscopy (AFM) and fluorescence observations, is proposed to visualize the insertion of glycosylphosphatidyl inositol (GPI) anchored alkaline phosphatase from buffer solutions into supported phospholipid bilayers. The technique involves use 27 nm diameter fluorescent latex beads covalently coupled amine groups proteins. Fluorescence allows estimation relative protein coverage membrane also introduces a height amplification for detection...
Influenza virus infection is a serious public health problem in the world, and understanding molecular mechanisms involved viral replication crucial. In this paper, we used minimalist approach based on lipid bilayer supported mica, which imaged by atomic force microscopy (AFM) physiological buffer, to analyze different steps of influenza fusion, from interaction intact viruses with their complete fusion. Our results show that sialic acid recognition priming upon acidification are sufficient...