A5034873788- Mosquito-borne diseases and control
- Malaria Research and Control
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Vibrio bacteria research studies
- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Vector-Borne Animal Diseases
- Immunotherapy and Immune Responses
- Toxin Mechanisms and Immunotoxins
- Bacteriophages and microbial interactions
- Cytomegalovirus and herpesvirus research
- Parvovirus B19 Infection Studies
- Invertebrate Immune Response Mechanisms
- Parasites and Host Interactions
- Immune Response and Inflammation
- Asthma and respiratory diseases
- Vector-borne infectious diseases
- Respiratory viral infections research
- Parasite Biology and Host Interactions
- Animal Virus Infections Studies
- Virology and Viral Diseases
- COVID-19 epidemiological studies
Sanofi (France)
2011-2022
Institut Mérieux (France)
1999-2015
Laboratoire de Biologie et Modélisation de la Cellule
1994-1995
Université Claude Bernard Lyon 1
1994-1995
Human rhinovirus (RV) infections are the principle cause of common colds and precipitate asthma COPD exacerbations. There is currently no RV vaccine, largely due to existence ∼150 strains. We aimed define highly conserved areas proteome test their usefulness as candidate antigens for a broadly cross-reactive using mouse infection model. Regions VP0 (VP4+VP2) capsid protein were identified having high homology across RVs. Immunization with recombinant combined Th1 promoting adjuvant induced...
Significance Dengue virus (DENV) vaccine development is complicated by the existence of four genetically diverse DENV serotypes. A high degree antigenic match between strains and circulating DENVs may be important to achieve efficacy (VE). Using data from two phase 3 trials CYD-TDV vaccine, we assessed whether how VE against virologically confirmed dengue varied with amino acid sequence characteristics genotypes disease-causing DENVs. decreased dissimilarity insert After accounting for...
This study defined the genetic epidemiology of dengue viruses (DENV) in two pivotal phase III trials tetravalent vaccine, CYD-TDV, and thereby enabled virus genotype-specific estimates vaccine efficacy (VE). Envelope gene sequences (n = 661) from 11 DENV genotypes 10 endemic countries provided a contemporaneous global snapshot population genetics revealed high amino acid identity between E genes strains wild-type trial participants, including at epitope sites targeted by neutralising human...
Zika virus (ZIKV) is an emerging mosquito-borne pathogen representing a global health concern. It has been linked to fetal microcephaly and other birth defects neurological disorders in adults. Sanofi Pasteur engaged the development of inactivated ZIKV vaccine, as well live chimeric vaccine candidate ChimeriVax-Zika (CYZ) that could become preferred depending on future epidemiology. This report focuses CYZ was constructed by replacing pre-membrane envelope (prM-E) genes genome attenuated...
Yellow fever (YF) remains a threat to human health in tropical regions of Africa and South America. Live-attenuated YF-17D vaccines have proven be safe effective protecting travellers populations endemic against YF, despite very rare severe reactions following vaccination — YF vaccine-associated viscerotropic disease (YEL-AVD) neurological (YEL-AND). We describe the generation selection live-attenuated vaccine candidate present its preclinical profile. Initially, 24 sub-strains from...
Helper CD4(+) T cells presumably play a major role in controlling cytomegalovirus (CMV) by providing help to specific B and CD8(+) cytotoxic cells, as well through cytotoxicity-mediated mechanisms. Since CMV glycoprotein (gB) is candidate for subunit vaccine against CMV, we searched gB-epitopes presented human leukocyte antigen (HLA)-class II molecules.Dendritic obtained from CMV-seropositive donors were loaded with recombinant gB co-cultured autologous cells. Microcultures that specifically...
The recombinant yellow fever-17D-dengue virus, live, attenuated, tetravalent dengue vaccine (CYD-TDV) is licensed in several dengue-endemic countries. Although the provides protection against dengue, level of differs by serotype and warrants further investigation. We characterized antigenic properties each virus using highly neutralizing human monoclonal antibodies (hmAbs) that bind quaternary structure-dependent epitopes. Specifically, we monitored binding virus-1 (DENV-1; 1F4), DENV-2...
Human T cell leukaemia virus type I (HTLV-I) is a direct activator of human resting lymphocytes. The present study was undertaken to delineate further the role viral particles and define involvement envelope glycoproteins in induction mitogenic stimulation. Virus-producing cells treated with paraformaldehyde (PFA) were found be unable induce formation syncytia, but still able trigger proliferation cells. Likewise, PFA-treated mitogenic. These results suggest that event triggered before...
The envelope (env) gene of human T cell leukaemia virus type I (HTLV-I) was inserted into an expression vector, referred to as phMTenv, under the transcriptional control metallothionein IIa promoter (hMT-IIa). When this vector transiently transfected in HeLa cells treated with hMT-IIa inducers, formation multinucleated observed, indicating functional surface and transmembrane glycoproteins. Of several clones phMTenv together a plasmid carrying neomycin resistance isolated after selection...
Although the enzyme-linked immunosorbent assay (ELISA) is well established for quantitating epitopes on inactivated virions used as vaccines, it less suited detecting potential overlaps between recognized by different antibodies raised against virions. We fluorescent correlation spectroscopy (FCS) to detect 3 monoclonal (mAbs 4B7-1H8-2E10, 1E3-3G4, 4H8-3A12-2D3) selected their ability specifically recognize poliovirus type 3. Competition of Alexa488-labeled mAbs with non-labeled revealed...