Abstract A first group of three horses was given diet 1 (D1) allowing 1180 g per 100 kg body weight (BW) a pelleted food rich in fibre (P1) and 556 BW straw during 20-day period to allow for adaptation. second four were 2 (D2) cereals (P2) 1000 meadow hay the same period. Digesta collected from stomach, duodenum, jejunum, ileum, caecum, right ventral colon, left dorsal small faeces under general anaesthesia 2·5 h after ingestion morning meal. The concentration total anaerobic, cellulolytic...

Dirofilaria immitis, D. repens and Acanthocheilonema reconditum are the main causative agents of zoonotic canine filariosis.We developed a combined multiplex approach for filaria Wolbachia detection using 28S-based pan-filarial 16S-based pan-Wolbachia qPCRs, respectively, involving fast typing method positive samples triplex qPCR targeting A. reconditum, immitis repens, duplex repens. The was complemented by differential diagnosis heartworms (D. Angiostrongylus vasorum) cox1 ftsZ PCRs to...

Our knowledge of the microflora stomach horse is still limited, although some data indicate its important role in nutrition. The objective this experiment was to investigate microbial and biochemical profiles quantify disappearance dietary starch. Total anaerobic bacteria, lactate-utilizing lactobacilli, streptococci were determined, characteristics (pH, DM, D- L-lactate, D-glucose, NH3, VFA concentrations) measured chyme collected from 4 horses by naso-gastric intubation aided endoscopy, at...

This study aimed at assessing the impact of four barley forms on total tract apparent digestibility dietary fibre in horses fed a large amount starch morning meal (0.27% BW). Processed had greater pre-caecal than whole form. Based this result, we hypothesised that using barley-processing methods would limit potential dumping undegraded hindgut and, consequently, negative effect degradation hindgut. In 4×4 latin square design, mature geldings fitted with right ventral colon-fistula were...

Abstract The study reported in this paper was conducted to evaluate the digestibility of dietary carbohydrates (‘starch and sugars’ (S), neutral-detergent fibre (NDF), acid-detergent (ADF)) organic matter (OM) different parts equine digestive tract (stomach, jejuno-ileum, caecum, right ventral colon, left dorsal small colon faeces). Three horses were given a standard diet (D1) based on fibrous pellets straw four offered high energy (D2) starch meadow hay. digesta collection procedure, by...

These guidelines are intended to provide an in-depth review of current knowledge and assist the planning implementation studies for evaluating efficacy parasiticides in reducing transmission vector-borne pathogens (VBPs) dogs cats. At present, prevention VBP companion animals is generally achieved through administration products that can repel or rapidly kill arthropods, thus preventing interrupting feeding before occurs. The present complement existing guidelines, which focus on assessment...

Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) is an emerging tool for routine identification of bacteria, archaea and fungi. It has also been recently applied as accurate approach arthropod identification. Preliminary studies have shown that the MALDI-TOF MS was able to differentiate whether ticks mosquitoes were infected or not with some bacteria Plasmodium parasites, respectively. The aim present study test efficiency in distinguishing protein...

This study assessed the influence of a topical ectoparasiticide (dinotefuran-permethrin-pyriproxyfen, DPP, Vectra® 3D, Ceva Animal Health) combined with macrocyclic lactone (milbemycin oxime, MBO, Interceptor®, Virbac) on transmission heartworm L3 from mosquitoes to dogs and subsequent development worms in treated exposed infected mosquitoes. Thirty-two beagle were allocated four groups eight: Group 1, untreated controls; 2, topically DPP Day 0; 3, orally MBO 51; 4, 0 51. Dogs under sedation...

This study was designed to assess the ability of fed male Dermacentor reticulatus ticks transmit Babesia canis dogs after being detached from previous canine or ovine hosts.The an exploratory, parallel group design conducted in two trials. All animals were sero-negative for babesiosis prior enrolment. In a first trial, donor and sheep infested with infected uninfected female 72 h. The second host 24 h before tick removal. experiment repeated but 88 8 After infestation, maintained under...

Dirofilaria immitis and repens are the main causative agents of heartworm disease subcutaneous dirofilariasis in domestic wild canids, respectively. Both pathogens have zoonotic potential transmitted by mosquitoes. The present study aimed to determine transmission period, prevalence diversity spp. vectors from endemic areas Corsica (France).A monthly point data model based on average temperature recorded four meteorological stations during 2017 was used calculate period. From June September...

Summary Reasons for performing study: In endurance horses, commonly fed 80% forage, energy is provided mainly by VFA produced in the hindgut during cell wall degradation, but digestibility has been reported to be impaired exercise. Objectives: To assess influence of a long‐term conditioning on digestion horses. Methods: The total tract apparent dry matter, organic matter and fibrous constituents, solid liquid mean retention time faecal microbial ecosystem purebred Arabian horses were...

Kinetoplastids are protozoa containing a range of ubiquitous free_living species–pathogens invertebrates, vertebrates and even some plants. Some them causative agents canine vector-borne diseases. Their diagnosis is often missing in gold standard. Here, we proposed molecular approach for the study Kinetoplastida. The TaqMan qPCR assays target following genes: 24Sa LSU Kinetoplastida, 28S Leishmania/ Trypanosoma spp., 5.8S rRNA 18S SSU Leishmania kinetoplast minicircle DNA (kDNA) L. donovani...

This study was designed to compare the efficacy of two ectoparasiticides against adult fleas on dogs: a topical (DPP, dinotefuran-permethrin-pyriproxyfen) and systemic (S, spinosad). Dogs (n = 48; 10.21-22.86 kg BW) were allocated six groups eight dogs each (C1, C4, DPP1, DPP4, S1, S4). in treated administered (3.6 mL DPP) or tablet (665 1040 mg S) day 0. Infestations with 100 unfed (Ctenocephalides felis) occurred days -6, -1, 2, 7, 14, 21 28. An additional untreated group (QC, n 6)...

In dogs, tick infestation can cause damage ranging from a simple skin irritation to severe diseases and/or paralysis leading animal death. For example, Ixodes ricinus and I. scapularis are among the species incriminated most in transmission of Borrelia burgdorferi, agent human canine Lyme borreliosis (LB). this study, we aimed compare efficacy two products designed for dogs—an oral systemic ectoparasiticide topical repellent ectoparasiticide—against acquisition B. burgdorferi by adult using...

This study was designed to compare the efficacy of three topical combinations on dogs in outdoor conditions against adult cat fleas (Ctenocephalides felis), flea egg hatch and emergence, brown dog ticks (Rhipicephalus sanguineus sensu lato). Treatment performed day 0 with a placebo; dinotefuran, pyriproxifen permethrin (DPP); fipronil (S)-methoprene (FM) or imidacloprid (IP). Dogs (n = 32), housed outdoors for 7 months, were treated monthly four consecutive months (on days 0, 30, 60 90)...

This study assessed the influence of a topical ectoparasiticide (dinotefuran-permethrin-pyriproxyfen, DPP, Vectra®3D, Ceva Animal Health) on acquisition heartworm microfilariae by mosquitoes exposed to microfilaremic dogs weekly for 1 month.Six beagle (9.2 ± 1.6 kg body weight) infected with Dirofilaria immitis were allocated two groups three dogs: an untreated control group and DPP-treated group. Dogs treated Day 0 under sedation h 80 20 unfed Aedes aegypti. Each dog was released into...

Leishmaniasis is among the world’s most neglected diseases. Dogs are main reservoirs/hosts of Leishmania infantum , causative agent both canine and human visceral leishmaniosis. Canine leishmaniasis (CanL) represents a public health problem as one prevalent zoonotic diseases worldwide. Current therapeutics present drawbacks; thus, there need for more effective, safer, cheaper drugs. The aim this study was to evaluate compare efficacy oral administration artesunate or meglumine...

ABSTRACT: To determine prececal starch digestibili-ty and estimate glucose uptake from the digestion of 4 forms barley in small intestine, mature cecally fistulated geldings (449 ± 41 kg BW) fed a 62:38 (wt/wt) meadow hay:concentrate diet at 1.7 DM/100 BW were included × Latin square design experiment. During each period, horses received 80% DM their concentrate as 1 same batch barley, whole grain, 2.5 mm ground, steam flaked, pelleted. Hay was offered 2 equal meals unequal meals. The supply...

This study was designed to assess the onset of therapeutic and residual insecticidal efficacy a topical ectoparasiticide (Vectra(®)3D, DPP) on dogs over one month against cat flea, Ctenocephalides felis felis. Adult (n=32, 11.0-18.7 kg) were infested with 100 adult fleas days -6, -2, 7, 14, 21 28. Based flea retention, allocated in two groups treated topically day 0 control solution (CS) or DPP. Each group divided subgroups which live counted by combing 2 6h after treatment at each...

This study was designed to compare the therapeutic and residual efficacy for 1 month of three topical ectoparasiticides on mixed-bred dogs against brown dog tick, Rhipicephalus sanguineus. Adult (n = 32, 10.8-18.4 kg BW) were allocated 4 groups 8) infested with 50 adult ticks days -8, -2, 7, 14, 21, 28. Within each group, treated topically day 0 a control solution (CS), Vectra 3D (DPP), Frontline Plus (FM), or K9 Advantix (IP). Ticks enumerated 24 h after treatment subsequent tick...