A5058840373- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Marine Bivalve and Aquaculture Studies
- Coral and Marine Ecosystems Studies
- Aquaculture disease management and microbiota
- Invertebrate Immune Response Mechanisms
- Helminth infection and control
- French Urban and Social Studies
- Agriculture and Rural Development Research
- Antimicrobial Peptides and Activities
- Marine Sponges and Natural Products
- Food Supply Chain Traceability
- Marine and fisheries research
- Biological Research and Disease Studies
- Genetics, Aging, and Longevity in Model Organisms
- Digital Marketing and Social Media
- Aquatic Invertebrate Ecology and Behavior
- Vibrio bacteria research studies
- Aging, Elder Care, and Social Issues
- Ocean Acidification Effects and Responses
- Energy and Environmental Systems
- Marine and coastal plant biology
- Trace Elements in Health
- Epigenetics and DNA Methylation
- Research on Leishmaniasis Studies
Labex Corail
2022-2025
Ifremer
2016-2025
University of French Polynesia
2020-2025
Institut de Recherche pour le Développement
2018-2025
Institut Louis Malardé
2023-2025
Université de Perpignan
2015-2024
Ecosystèmes Insulaires Océaniens
2022-2024
Université de Montpellier
2000-2024
Centre National de la Recherche Scientifique
2015-2024
Interaction Hôtes Agents Pathogènes
2013-2022
Abstract Biomphalaria snails are instrumental in transmission of the human blood fluke Schistosoma mansoni . With World Health Organization's goal to eliminate schistosomiasis as a global health problem by 2025, there is now renewed emphasis on snail control. Here, we characterize genome glabrata, lophotrochozoan protostome, and provide timely important information biology. We describe aspects phero-perception, stress responses, immune function regulation gene expression that support...
Infectious diseases are mostly explored using reductionist approaches despite repeated evidence showing them to be strongly influenced by numerous interacting host and environmental factors. Many with a complex aetiology therefore remain misunderstood. By developing holistic approach tackle the complexity of interactions, we decipher intra-host interactions underlying Pacific oyster mortality syndrome affecting juveniles Crassostrea gigas, main species exploited worldwide. Using experimental...
We report here the isolation of two isoforms a novel cysteine‐rich peptide from haemocytes (isoform A 4.438 Da and B 4.562 Da) plasma A) mussel, Mytilus galloprovincialis . The molecules display antibacterial activity against gram‐positive bacteria, whereas only isoform is active fungus Fusarium oxysporum gram‐negative bacteria Escherichia coli D31. Complete sequences were determined by combination Edman degradation, mass spectrometry cDNA cloning using haemocyte library. mature molecules,...
Many parasites modify their host behaviour to improve own transmission and survival, but the proximate mechanisms remain poorly understood. An original model consists of parasitoid Dinocampus coccinellae its coccinellid host, Coleomegilla maculata ; during manipulation, is not in contact with anymore. We report herein discovery characterization a new RNA virus ( D. paralysis virus, DcPV). Using combination RT-qPCR electron microscopy, we demonstrate that DcPV stored oviduct females,...
Scleractinian corals are the most basal eumetazoan taxon and provide biological physical framework for coral reefs, which among diverse of all ecosystems. Over past three decades coincident with climate change, these phototrophic symbiotic organisms have been subject to increasingly frequent severe diseases, now geographically widespread a major threat Although immunity has increasing study, available information remains fragmentary, especially respect antimicrobial responses. In this we...
Discoveries made over the past ten years have provided evidence that invertebrate antiparasitic responses may be primed in a sustainable manner, leading to failure of secondary encounter with same pathogen. This phenomenon called "immune priming" or "innate immune memory" was mainly phenomenological. The demonstration this process remains obtained and underlying mechanisms remain discovered exhaustively tested rigorous functional molecular methods, eliminate all alternative explanations. In...
Although the term holobiont has been popularized in corals with advent of hologenome theory evolution, underlying concepts are still a matter debate. Indeed, relative contribution host and environment especially thermal regime shaping microbial communities should be examined carefully to evaluate potential role symbionts for adaptation context global changes. We used sessile, long-lived, symbiotic environmentally sensitive reef-building coral Pocillopora damicornis address these issues....
In the last decade, important discoveries have shown that resistance to reinfection can be achieved without a functional adaptive immune system, introducing concept of innate memory in invertebrates. However, this field has been constrained by limited number molecular mechanisms evidenced support these phenomena. Taking advantage an invertebrate species, Pacific oyster ( Crassostrea gigas ), which we one longest and most effective periods protection against viral infection observed...
Abstract Background The interaction of organisms with their surrounding microbial communities influences many biological processes, a notable example which is the shaping immune system in early life. In Pacific oyster, Crassostrea gigas , role environmental community on maturation — and, importantly, protection from infectious disease still an open question. Results Here, we demonstrate that life exposure durably improves oyster survival when challenged pathogen causing mortality syndrome...
MGD1 (Mytilus galloprovincialis defensin 1), a new member of the arthropod family, is 4 kDa antibacterial peptide previously isolated from plasma Mediterranean mussels. We report here presence in organelle-rich fraction hemocytes and cDNA sequence corresponding to one isoform mRNA: MGD2. Sequence analysis indicated that MGDs are synthesised as precursors consisting putative signal 21 residues, active 39 amino acids residue carboxyl-terminal extension, rich acidic acids. Localisation...
Four cationic peptides were purified from mussel (Mytilus galloprovincialis) hemocytes. A combination of Edman degradation and mass spectrometry plasma revealed (i) a previously characterized molecule, mytilin B (Charlet, M., Chernysh, S., Philippe, H., Hetrut, C., Hoffmann, J., Bulet, P. (1996) J. Biol. Chem. 271, 21808-21813) (ii) three new isoforms, C, D, G1. The four molecules exhibited complementary antimicrobial properties. cDNA sequence coding for the precursor was obtained hemocyte...
ABSTRACT In previous papers, we characterised 3 types of 4-kDa, cysteine-rich, cationic antimicrobial peptides: MGDs (for Mytilus galloprovincialis defensins), mytilins and myticins, which are abundant in the mussel hemocytes. present work, revealed a differential distribution cells expressing different genes. addition, using confocal electron microscopy, confirmed that defensins were partially located sub-types circulating hemocytes although peptides can be same cell, even granule. We also...
Important biological activities could be affected in metal exposed species, and among the main physiological functions, immunity may provide one (or more) effector(s) which expression can directly by a exposure various macroinvertebrates. As many proteinic effectors showed high degree of homology between we have developed PCR approach to characterize partial mRNA sequences selected laboratory model, Eisenia fetida. After cloning, levels each gene were analyzed following exposures (80 800...
Abstract Background Coral bleaching can be defined as the loss of symbiotic zooxanthellae and/or their photosynthetic pigments from cnidarian host. This major disturbance reef ecosystems is principally induced by increases in water temperature. Since beginning 1980s and onset global climate change, this phenomenon has been occurring at increasing rates scales, with severity. Several studies have undertaken last few years to better understand cellular molecular mechanisms coral but jigsaw...
For many decades, invertebrate immunity was believed to be non-adaptive, poorly specific, relying exclusively on sometimes multiple but germ-line encoded innate receptors and effectors. But recent studies performed in different species have shaken this paradigm by providing evidence for various types of somatic adaptations at the level putative immune leading an enlarged repertoire recognition molecules. Fibrinogen Related Proteins (FREPs) from mollusc Biomphalaria glabrata are example these...