A5101902583- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Advanced Fluorescence Microscopy Techniques
- Advanced Biosensing Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Nanoplatforms for cancer theranostics
- Biosensors and Analytical Detection
- Advancements in Transdermal Drug Delivery
- Quantum Dots Synthesis And Properties
- Acne and Rosacea Treatments and Effects
- Photodynamic Therapy Research Studies
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Nanocluster Synthesis and Applications
- Advanced Nanomaterials in Catalysis
- Bee Products Chemical Analysis
- Photoacoustic and Ultrasonic Imaging
- Optical Coherence Tomography Applications
- Nanoparticle-Based Drug Delivery
- Radiopharmaceutical Chemistry and Applications
- Advanced Electron Microscopy Techniques and Applications
- Single-cell and spatial transcriptomics
- Chemical Synthesis and Analysis
- Ultrasound and Hyperthermia Applications
- Orthopaedic implants and arthroplasty
Massachusetts General Hospital
2017-2022
Harvard University
2017-2022
Seoul National University of Education
2010-2021
Seoul National University
2014-2017
Government of the Republic of Korea
2017
University of Seoul
2014-2015
Seoul National University Hospital
2015
Abstract For the effective application of surface‐enhanced Raman scattering (SERS) nanoprobes for in vivo targeting, tissue transparency probe signals should be as high it can order to increase detection sensitivity and signal reproducibility. Here, near‐infrared (NIR)‐sensitive SERS (NIR dots) are demonstrated multiplex detection. The NIR dots consist plasmonic Au/Ag hollow‐shell (HS) assemblies on surface silica nanospheres simple aromatic labels. diameter HS interior is adjusted from 3 11...
We explored a single-step approach for the rapid growth of Ag nanoshells (Ag NSs) under mild conditions. Without predeposition seed metals, uniform and complete layer shells was rapidly formed on silica core particles within 2 min at 25 °C via single electron transfer from octylamine to Ag+ ions. The size thickness NSs were effectively tuned by adjusting concentration nanoparticles (silica NPs) with optimal concentrations AgNO3 octylamine. This unusually attributed significant increase in...
Abstract Optical endoscopic imaging, which was recently equipped with bioluminescence, fluorescence and Raman scattering, allows minimally invasive real-time detection of pathologies on the surface hollow organs. To characterize pathologic lesions in a multiplexed way, we developed dual modal fluorescence-Raman endomicroscopic system (FRES), used surface-enhanced scattering nanoprobes (F-SERS dots). Real-time, vivo multiple target specific cancer successful, based fast imaging capability...
Based on AgNSs with bump-structures, the NIR active, ultrasensitive, and biocompatible SERS probes were developed applied for<italic>in vivo</italic>cell tracking in a live animal.
The most superficial layer of the epidermis, stratum corneum, plays a crucial role in retaining hydration; if its structure or composition is compromised, dry skin may result as consequence poor water retention. Dry typically treated with topical application humectant agents that attract into skin. Corneometry, industry standard for measuring hydration, works by assessing bulk electrical properties However, this technique samples large volume tissue and thus does not resolve biochemical...
We developed highly sensitive surface-enhanced Raman scattering (SERS) probes based on SiO<sub>2</sub>@Au@Ag nanoparticles (NPs) using the Ag growth onto Au NP seeds method.
Antibody-conjugated nanoparticles (NPs) have attracted great attention in diagnostic and therapeutic applications due to their high sensitivity specificity for biotargets, as well wide applicability. Unfortunately, these features are significantly affected by antibody conjugation methods terms of efficiency, orientation the target binding site antibody, denaturation during chemical reactions. Furthermore, number conjugated antibodies on each NP overall targeting efficacy critical factors a...
Fluorescence endomicroscopy provides quick access to molecular targets, while Raman spectroscopy allows the detection of multiple targets. Using a simultaneous fluorescence-Raman endoscopic system (FRES), we herein demonstrate its potential in cancer diagnosis an orthotopically induced colorectal (CRC) xenograft model. In model, epidermal growth factor receptor (EGFR) and vascular endothelial (VEGF) were targeted with antibody-conjugated fluorescence surface-enhanced scattering (F-SERS)...
Autofluorescence from endogenous biomolecules presents a significant challenge in immunofluorescence microscopy, often severely hindering the detection of specific fluorescence signals. While various techniques, including chemical- and quencher-based methods, photobleaching, digital-based protocols like autofluorescence image subtraction, have been developed to suppress autofluorescence, each approach has inherent limitations that restrict their efficacy across different tissue types....
Peptides synthesized on microbeads were encoded by chemically and physically adsorbing surface-enhanced Raman spectroscopic nanoparticles (SERS dots) the microbead surface during synthesis, which could be easily rapidly decoded spectroscopy.
Surface‐enhanced Raman scattering ( SERS ) has attracted considerable interest as a sensitive vibration‐specific probe for bioanalytical and imaging applications. Among the various bioprobes available, Ag‐embedded tags have been rigorously developed an extensive range of biodetection In this review, we look at additional functionality that can offer via its magnetic properties, fluorescence, extension optical region into near‐infrared NIR spectrum. Such be achieved by using Ag nanoparticles...
Abstract Recently, preparation and screening of compound libraries remain one the most challenging tasks in drug discovery, biomarker detection biomolecular profiling processes. So far, several distinct encoding/decoding methods such as chemical encoding, graphical encoding optical have been reported to identify those libraries. In this paper, a simple efficient surface-enhanced Raman spectroscopic (SERS) barcoding method using highly sensitive SERS nanoparticles (SERS ID) is presented. The...
Abstract Understanding a drug candidate’s pharmacokinetic (PK) parameters is challenging but essential aspect of development. Investigating the penetration and distribution topical drug’s active pharmaceutical ingredient (API) allows for evaluating delivery efficacy, which necessary to ensure viability. A gel (BPX-05) was recently developed treat moderate severe acne vulgaris by directly delivering combination antibiotic minocycline retinoid tazarotene pilosebaceous unit dermis. In order...
Acne vulgaris is a common chronic skin disease in young adults caused by infection of the pilosebaceous unit, resulting pimples and possibly permanent scarring on skin.Minocycline, antibiotic, has been widely utilized as systemic antimicrobial treatment for acne via oral administration.Recently, topical minocycline gel (BPX-01) was developed to directly deliver through epidermis into unit achieve localized with lower doses drug.As effectiveness drug related its successful delivery, there...
We present a template-assisted method for synthesizing nanogap shell structures biomolecular detections based on surface-enhanced Raman scattering. The interior nanogap-containing silver structure, referred to as (Ag NGS), was fabricated nanoparticles NPs)-coated silica, by adsorbing small aromatic thiol molecules the Ag NPs. NGSs showed high enhancement factor and good signal uniformity, using 785-nm excitation. performed in vitro immunoassays prostate-specific antigen model cancer...
Abstract Immunotargeting ability of antibodies may show significant difference between in vitro and vivo. To select antibody leads with high affinity specificity, it is necessary to perform vivo validation candidates following screening. Herein, a robust anti‐tetraspanin‐8 against human colon cancer using ratiometric quantification method reported. The performed on single mouse analyzed by multiplexed surface‐enhanced Raman scattering ultrasensitive near infrared (NIR)‐active resonance...
To impart a desired optical property to metal nanoparticles (NPs) suitable for surface-enhanced Raman scattering (SERS) applications, it is crucial assemble them in two or three dimensions addition controlling their size and shape. Herein, we report new strategy the synthesis direct assembly of Ag NPs on silica nanospheres (AgNPs-SiNS) presence poly(ethylene glycol) (PEG) derivatives such as PEG-OH, bis(amino)-PEGs (DA-PEGs), O,O'-bis(2-aminopropyl)PEG (DAP-PEG). They exhibited different...
In pharmacokinetic studies of topical drugs, fluorescence microscopy methods can enable the direct visualization and quantification fluorescent drugs within skin.One potential limitation this approach, however, is strong endogenous skin tissues that makes straightforward identification specific drug molecules challenging.To study effect quantify in context pharmacokinetics, an integrating sphere-based screening tool was designed to collect yield data from human specimens.Such information...
Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub>@Au nanoparticles (NPs) that have plasmonic and magnetic properties were prepared by simple immobilization method of Au NPs to silica coated NPs.
Immunohistochemistry is a crucial method for detecting specific proteins within tissue samples, yet constrained to one biomarker per section. Multiplexed immunofluorescence, while allowing simultaneous visualization of multiple proteins, faces limitations in the number fluorescent labels due spectral overlap. Although cyclic immunofluorescence techniques have successfully broadened antibody staining capacities single sample, they are plagued by time-consuming and labor-intensive procedures,...