A5046273697- SARS-CoV-2 and COVID-19 Research
- Gut microbiota and health
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Dietary Effects on Health
- Heparin-Induced Thrombocytopenia and Thrombosis
- COVID-19 Impact on Reproduction
- PARP inhibition in cancer therapy
- Tryptophan and brain disorders
- vaccines and immunoinformatics approaches
- Diet and metabolism studies
- Cancer, Stress, Anesthesia, and Immune Response
- Immune responses and vaccinations
- Platelet Disorders and Treatments
- Immune Cell Function and Interaction
Flinders Medical Centre
2021-2023
South Australian Health and Medical Research Institute
2021-2023
Flinders University
2021-2023
Identifying the molecular mechanisms that promote optimal immune responses to coronavirus disease 2019 (COVID-19) vaccination is critical for future rational vaccine design. Here, we longitudinally profile innate and adaptive in 102 adults after first, second, third doses of mRNA or adenovirus-vectored COVID-19 vaccines. Using a multi-omics approach, identify key differences induced by ChAdOx1-S BNT162b2 correlate with antigen-specific antibody T cell reactogenicity. Unexpectedly, observe...
Studies investigating whether there is a causative link between the gut microbiota and lifespan have largely been restricted to invertebrates or mice with reduced because of genetic deficiency. We investigate effect early-life antibiotic exposure on otherwise healthy, normal chow-fed, wild-type mice, monitoring these for more than 700 days in comparison untreated control mice. demonstrate emergence two different low-diversity community types, post-antibiotic (PAM) I PAM II, following...
Immune agonist antibodies (IAAs) are promising immunotherapies that target co-stimulatory receptors to induce potent anti-tumor immune responses, particularly when combined with checkpoint inhibitors. Unfortunately, their clinical translation is hampered by serious dose-limiting, immune-mediated toxicities, including high-grade and sometimes fatal liver damage, cytokine release syndrome (CRS), colitis. We show the immunotoxicity, induced IAAs anti-CD40 anti-CD137, dependent on gut...
We present this protocol using a mouse model to assess the impact of early-life antibiotic exposure on mammalian lifespan and composition gut microbiota over time. describe longitudinal fecal sampling health monitoring following exposure. detail DNA extraction 16S rRNA gene sequencing longitudinally profile microbiota. Finally, we discuss how address potential confounders such as stochastic recolonization For complete details use execution protocol, please refer Lynn et al. (2021).
Abstract We longitudinally profiled immune responses in 102 adults who received BNT162b2 (Pfizer-BioNTech) or ChAdOx1-S (Oxford-AstraZeneca) as their primary vaccinations. Bloods were collected pre-vaccination, 1-7 days after the 1 st , 2 nd and 3 rd doses (BNT162b2 mRNA-1273) to assess innate early adaptive responses, ∼28 immunogenicity. Using a multi-omics approach including RNAseq, cytokine multiplex assay, proteomics, lipidomics, flow cytometry we identified key differences induced by...
Despite promising preclinical and clinical data demonstrating that immune agonist antibody immunotherapies (IAAs) such as αOX40 induce strong antitumor responses, translation has been significantly hampered by the propensity of some IAAs to dose-limiting sometimes life-threatening immunotoxicities cytokine release syndrome hepatotoxicity. For example, in a recent study was shown significant liver damage mice inducing pyroptosis natural killer T cells (NKT) cells. Surprisingly; however, given...