A5060148381- Immune cells in cancer
- COVID-19 Clinical Research Studies
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Peroxisome Proliferator-Activated Receptors
- Single-cell and spatial transcriptomics
- Tuberculosis Research and Epidemiology
- Cancer, Lipids, and Metabolism
- Cancer Immunotherapy and Biomarkers
- Adipose Tissue and Metabolism
- Fatty Acid Research and Health
- CAR-T cell therapy research
- Inflammatory mediators and NSAID effects
- Inflammasome and immune disorders
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Chemokine receptors and signaling
University of Colorado Anschutz Medical Campus
2024-2025
Brigham and Women's Hospital
2019-2022
Harvard University
2019-2022
Broad Institute
2019-2021
University of Massachusetts Boston
2021
Massachusetts Institute of Technology
2019
Abstract Background Immunosuppressive and anti-cytokine treatment may have a protective effect for patients with COVID-19. Understanding the immune cell states shared between COVID-19 other inflammatory diseases established therapies help nominate immunomodulatory therapies. Methods To identify cellular phenotypes that be across tissues affected by disparate diseases, we developed meta-analysis integration pipeline models removes effects of technology, tissue origin, donor confound cell-type...
Background Treatment with immunotherapy can elicit varying responses across cancer types, and the mechanistic underpinnings that contribute to response vrsus progression remain poorly understood. However, date there are few preclinical models accurately represent these disparate disease scenarios. Methods Using combinatorial radio-immunotherapy consisting of PD-1 blockade, IL2Rβγ biased signaling, OX40 agonism we were able generate tumor conflicting responses, where head neck squamous cell...
Immunosuppressive and anti-cytokine treatment may have a protective effect for patients with COVID-19. Understanding the immune cell states shared between COVID-19 other inflammatory diseases established therapies help nominate immunomodulatory therapies. Using an integrative strategy, we built reference by meta-analyzing > 300,000 cells from 5 including rheumatoid arthritis (RA), Crohn's disease (CD), ulcerative colitis (UC), lupus, interstitial lung disease. Our cross-disease analysis...
Abstract Purpose: Head and neck cancer (HNC) improvements are stagnant, even with advances in immunotherapy. Our previous clinical trial data show that altered fatty acid (FA) metabolism correlates outcome. We hypothesized pharmacologic dietary modulation of FA catabolism will affect therapeutic efficacy. Experimental Design: performed vivo vitro experiments using PPARα agonism fenofibrate (FF) or high oleic diets (OAD) radiotherapy, generating metabolomic, proteomic, stable isotope tracing,...
Clinically approved head and neck squamous cell carcinoma (HNSCC) immunotherapies manipulate the immune checkpoint blockade (ICB) axis but have had limited success outside of recurrent/metastatic disease. Interleukin-7 (IL7) has been shown to be essential for effector T-cell survival, activation, proliferation. Here, we show that IL7 in combination with radiotherapy (RT) is effective activating CD8 + T-cells reducing tumor growth. Our studies were conducted using both human papillomavirus...
Abstract Mycobacterium tuberculosis ( M . tb ) results in 10 million active (TB) cases and 1.5 deaths each year 1 , making it the world’s leading infectious cause of death 2 Infection leads to either an asymptomatic latent state or TB disease. Memory T cells have been implicated disease progression, but specific cell states involved not yet delineated because limited scope traditional profiling strategies. Furthermore, immune activation during infection confounds underlying differences...
Abstract Many non-coding genetic variants cause disease by modulating gene expression. However, identifying these expression quantitative trait loci (eQTLs) is complicated gene-regulation differences between cell states. T cells, for example, have fluid, multifaceted functional states in vivo that cannot be modeled eQTL studies aggregate cells. Here, we and eQTLs at single-cell resolution. Using >500,000 resting memory cells from 259 Peruvians, found over one-third of the 6,511 cis -eQTLs...
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<div>AbstractPurpose:<p>Head and neck cancer (HNC) improvements are stagnant, even with advances in immunotherapy. Our previous clinical trial data show that altered fatty acid (FA) metabolism correlates outcome. We hypothesized pharmacologic dietary modulation of FA catabolism will affect therapeutic efficacy.</p>Experimental Design:<p>We performed <i>in vivo</i> vitro</i> experiments using PPARα agonism fenofibrate (FF) or high oleic diets (OAD)...
<div>AbstractPurpose:<p>Head and neck cancer (HNC) improvements are stagnant, even with advances in immunotherapy. Our previous clinical trial data show that altered fatty acid (FA) metabolism correlates outcome. We hypothesized pharmacologic dietary modulation of FA catabolism will affect therapeutic efficacy.</p>Experimental Design:<p>We performed <i>in vivo</i> vitro</i> experiments using PPARα agonism fenofibrate (FF) or high oleic diets (OAD)...
Abstract Background Treatment with immunotherapy can elicit varying responses across cancer types, and the mechanistic underpinnings that contribute to response vs. progression remain poorly understood. However, date there are few preclinical models accurately represent these disparate disease scenarios. Methods Using combinatorial radio-immunotherapy consisting of PD-1 blockade, IL2Rβγ biased signaling, OX40 agonism we were able generate tumor conflicting responses, where head neck squamous...
T cell phenotyping is often limited by its reliance on single classes of markers (e.g., mRNA or protein). With multiview definitions states and their associations with non-immune factors, we can more precisely identify underlying disease outcomes. Here, use an integrative, multimodal strategy to characterize the landscape human memory cells. We computationally integrated high-dimensional single-cell RNA surface protein marker data produce atlas 500,089 cells from 259 individuals in a...