A5021599574- COVID-19 Clinical Research Studies
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- Cancer Immunotherapy and Biomarkers
- CRISPR and Genetic Engineering
- Acute Myeloid Leukemia Research
- Cytomegalovirus and herpesvirus research
- CAR-T cell therapy research
- Cancer Genomics and Diagnostics
- Pluripotent Stem Cells Research
- T-cell and B-cell Immunology
- Renal and related cancers
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Chronic Kidney Disease and Diabetes
- Circadian rhythm and melatonin
- Chemokine receptors and signaling
- Renal Diseases and Glomerulopathies
- Orthopedic Infections and Treatments
- Orthopaedic implants and arthroplasty
- Spaceflight effects on biology
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Rheumatoid Arthritis Research and Therapies
- Genetics, Aging, and Longevity in Model Organisms
Hospital for Special Surgery
2023-2024
Cornell University
2020-2023
Princess Margaret Cancer Centre
2018-2022
University Health Network
2018-2022
Abstract Background Immunosuppressive and anti-cytokine treatment may have a protective effect for patients with COVID-19. Understanding the immune cell states shared between COVID-19 other inflammatory diseases established therapies help nominate immunomodulatory therapies. Methods To identify cellular phenotypes that be across tissues affected by disparate diseases, we developed meta-analysis integration pipeline models removes effects of technology, tissue origin, donor confound cell-type...
Immune checkpoint inhibitor (ICI) therapies used to treat cancer, such as anti–PD-1 antibodies, can induce autoimmune conditions in some individuals. The T cell mechanisms mediating iatrogenic autoimmunity and their overlap with spontaneous diseases remain unclear. Here, we compared cells from the joints of 20 patients an inflammatory arthritis induced by ICI therapy (ICI-arthritis) two archetypal arthritides, rheumatoid (RA) psoriatic (PsA). Single-cell transcriptomic antigen receptor...
Abstract In the human hematopoietic system, rare self-renewing multipotent long-term stem cells (LT-HSCs) are responsible for lifelong production of mature blood and rational target clinical regenerative therapies. However, heterogeneity in cell compartment variable outcomes CRISPR/Cas9 editing make functional interrogation LT-HSCs challenging. Here, we report high efficiency LT-HSC at single-cell resolution using electroporation modified synthetic gRNAs Cas9 protein. Targeted short isoform...
Immunosuppressive and anti-cytokine treatment may have a protective effect for patients with COVID-19. Understanding the immune cell states shared between COVID-19 other inflammatory diseases established therapies help nominate immunomodulatory therapies. Using an integrative strategy, we built reference by meta-analyzing > 300,000 cells from 5 including rheumatoid arthritis (RA), Crohn's disease (CD), ulcerative colitis (UC), lupus, interstitial lung disease. Our cross-disease analysis...
Delayed detection of LN associates with worse outcomes. There are conflicting recommendations regarding a threshold level proteinuria at which biopsy will likely yield actionable management. This study addressed the association urine protein:creatinine ratios (UPCR) clinical characteristics and investigated incidence proliferative membranous histology in patients UPCR between 0.5 1.A total 275 SLE (113 first biopsy, 162 repeat) were enrolled multicentre multi-ethnic/racial Accelerating...
Abstract Immune checkpoint inhibitor (ICI) therapies that promote T cell activation have improved outcomes for advanced malignancies yet also elicit harmful autoimmune reactions. The mechanisms mediating these iatrogenic events remain unclear. Here we assayed cells from joints of patients affected by ICI-induced inflammatory arthritis (ICI-arthritis), which can present clinically indistinguishable rheumatoid (RA). Compared to the arthritides RA and psoriatic (PsA), ICI-arthritis contained an...
Abstract The I4 mission, the first all-civilian orbital flight investigated physiological effects of short duration spaceflight through a multi-omic approach. We analyzed telomere length, clonal hematopoiesis indeterminate potential (CHIP), whole genome stability, cell-free DNA (cfDNA) cell lysis, and immune gene expression. Our results revealed length dynamics similar to those observed in NASA Twins Study astronauts spending 6 months on ISS, with lengthening space shortening upon return...
Objective Diagnosis of periprosthetic joint infection (PJI) in patients with inflammatory arthritis (IA) is challenging, as features IA flares can mimic infection. We aimed to cross-sectionally determine if the optimal tests diagnose PJI osteoarthritis were present flares. Methods enrolled from October 2020 July 2022 3 groups: ( a ) PJI—total arthroplasty undergoing revision for infection, b Flare—IA flaring native joint, and c Aseptic—total aseptic revision. compared blood synovial fluid...
Abstract In the human hematopoietic system, rare self-renewing multi-potent long-term stem cells (LT-HSCs) are responsible for lifelong production of mature blood and rational target clinical regenerative therapies. However, heterogeneity in cell compartment variable outcomes CRISPR/Cas9 editing make functional interrogation LT-HSCs challenging. Here, we report high efficiency LT-HSC at single resolution using electroporation modified synthetic gRNAs Cas9 protein. Targeted short isoform...