A5003294920- Retinal Development and Disorders
- CRISPR and Genetic Engineering
- Mechanical Circulatory Support Devices
- Cardiac Structural Anomalies and Repair
- Aortic Disease and Treatment Approaches
- Transplantation: Methods and Outcomes
- Cardiac Arrest and Resuscitation
- Cardiac and Coronary Surgery Techniques
- Inflammatory Bowel Disease
- Cardiac Ischemia and Reperfusion
- Cardiac tumors and thrombi
- Advanced biosensing and bioanalysis techniques
- Infective Endocarditis Diagnosis and Management
- Cardiac Valve Diseases and Treatments
- Cardiac, Anesthesia and Surgical Outcomes
- Aortic aneurysm repair treatments
- Immune cells in cancer
- Pericarditis and Cardiac Tamponade
- Organ Transplantation Techniques and Outcomes
- Gut microbiota and health
- Atrial Fibrillation Management and Outcomes
- Retinal Diseases and Treatments
- Central Venous Catheters and Hemodialysis
- Coronary Artery Anomalies
- Genetics, Aging, and Longevity in Model Organisms
National Defense Medical Center
2016-2025
Tri-Service General Hospital
2016-2025
National Tsing Hua University
2015-2025
Columbia University
2015-2025
Instituto de Biología Molecular y Celular de Rosario
2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2025
National University of Rosario
2025
NewYork–Presbyterian Hospital
2015-2024
New York Hospital Queens
2024
Chang Gung University
2024
The U.S. Food and Drug Administration recently approved phase I/II clinical trials for embryonic stem (ES) cell-based retinal pigmented epithelium (RPE) transplantation, but this allograft transplantation requires lifelong immunosuppressive therapy. Autografts from patient-specific induced pluripotent (iPS) cells offer an alternative solution to problem. However, more data are required establish the safety efficacy of iPS in animal models before moving therapy into trials. This study...
Objective: Extracorporeal membrane oxygenation (ECMO) offers temporary haemodynamic support for those with refractory cardiogenic shock after cardiac surgery. We review our 5-year experience regarding ECMO use on who cannot be weaned from cardiopulmonary bypass analyse cases, predict the prognostic factors of survival and compare short-term medium-term results. Methods: From January 2002 to December 2006, 1764 patients underwent surgery in division. Among these, 51 (2.9%) required...
Massive parallel sequencing enables identification of numerous genetic variants in mutant organisms, but determining pathogenicity any one mutation can be daunting. The most commonly studied preclinical model retinitis pigmentosa called the "rodless" (rd1) mouse is homozygous for two mutations: a nonsense point (Y347X) and an intronic insertion leukemia virus (Xmv-28). Distinguishing which causes retinal degeneration still under debate nearly century after discovery this organism. Here, we...
Retinitis pigmentosa (RP) encompasses a diverse group of Mendelian disorders leading to progressive degeneration rods and then cones. For reasons that remain unclear, diseased RP photoreceptors begin deteriorate, eventually cell death and, consequently, loss vision. Here, we have hypothesized associated with mutations in phosphodiesterase-6 (PDE6) provokes metabolic aberration rod cells promotes the pathological consequences elevated cGMP Ca2+, which are induced by Pde6 mutation. Inhibition...
Strengthening the gut epithelial barrier is a potential strategy for management of microbiota-associated illnesses. Here, we demonstrate that dual-specificity phosphatase 6 (Dusp6) knockout enhances baseline colon integrity and ameliorates dextran sulfate sodium (DSS)-induced colonic injury. DUSP6 mutation in Caco-2 cells feature increases mitochondrial oxygen consumption, accompanied by altered glucose metabolism decreased glycolysis. We find Dusp6-knockout mice are more resistant to...
Pigmentation is one of the essential defense mechanisms against oxidative stress or UV irradiation; however, abnormal hyperpigmentation in human skin may pose a serious aesthetic problem. C-phycocyanin (Cpc) phycobiliprotein from spirulina and functions as an antioxidant light harvesting protein. Though it known that has been used to reduce hyperpigmentation, little literature addresses antimelanogenic mechanism Cpc. Herein, we investigated rationale for Cpc-induced inhibitory on melanin...
Hereditary retinal degenerative diseases, such as retinitis pigmentosa (RP), are characterized by the progressive loss of rod photoreceptors followed cones. While gene therapy clinical trials demonstrated temporary improvement in visual function, this approach has yet to achieve sustained functional and anatomical rescue after disease onset patients. The lack benefit could be due insufficient transduction efficiency viral vectors ("too little") and/or because is too advanced late") at time...
Subtherapeutic drug concentrations contribute to both primary and secondary nonresponse infliximab in children with Crohn disease (CD). The aim of this study was evaluate treatment outcomes at infusions 2 3 an objective establish targets during induction for responders.Single-center, prospective cohort anti- tumor necrosis factor-alpha naïve CD patients younger than 22 years starting infliximab. Clinical response defined the weighted pediatric activity index fourth infusion. Rates biological...
Standard-of-care infliximab dosing regimens were developed prior to the routine use of therapeutic drug monitoring and identification target concentrations. Not surprisingly, subtherapeutic concentrations in pediatric Crohn's disease (CD) are common. The primary aim was conduct a real-world pharmacokinetic (PK) evaluation discover blood biomarkers rapid clearance, identify exposure targets, secondary translate PK modeling clinic. In multicenter observational study, 671 peak trough from 78...
Obesity and type 2 diabetes have reached pandemic proportion. ALDH2 (acetaldehyde dehydrogenase 2, mitochondrial) is the key metabolizing enzyme of acetaldehyde other toxic aldehydes, such as 4-hydroxynonenal. A missense Glu504Lys mutation gene prevalent in 560 million East Asians, resulting reduced enzymatic activity. We find that male Aldh2 knock-in mice mimicking human were prone to develop diet-induced obesity, glucose intolerance, insulin resistance, fatty liver due adaptive...
Plastic films are widely used in current agricultural practices; however, most mulch discarded and buried the land after harvest, having adverse environmental impacts. To solve this problem, demand for biodegradable has been increasing recent years. Polybutylene succinate-co-adipate (PBSA) is a polymer with good ductility can be packaging mulching. In study, we isolated two elite fungal strains PBSA degradation from farmlands, i.e., Aspergillus fumigatus L30 terreus HC, latter showed better...
Mutations in rhodopsin (RHO) are the most common causes of autosomal dominant retinitis pigmentosa (adRP), accounting for 20% to 30% all cases worldwide. However, high degree genetic heterogeneity makes development effective therapies cumbersome. To provide a universal solution RHO-related adRP, we devised CRISPR-based, mutation-independent gene ablation and replacement (AR) compound therapy carried by dual AAV2/8 system. Moreover, developed novel hRHO
ABSTRACT Objectives The mortality rate of acute myocardial infarction (AMI)‐related refractory cardiogenic shock (rCS) remains high, particularly in patients experiencing cardiac arrest with extracorporeal cardiopulmonary resuscitation (ECPR). This study aimed to evaluate the outcomes early escalation CentriMag for AMI‐induced out‐of‐hospital (OHCA) ECPR. Methods Patients OHCA ECMO support after ECPR were enrolled. Clinical data analyzed identify predictive factors and survival benefits....
Abstract Hyperopia (farsightedness) is a common and significant cause of visual impairment, extreme hyperopia (nanophthalmos) consequence loss-of-function MFRP mutations. deficiency causes abnormal eye growth along the axis comorbidities, such as angle closure glaucoma, cystic macular edema, exudative retinal detachment. The Mfrp rd6 /Mfrp mouse used pre-clinical animal model degeneration, we found it was also hyperopic. To test effect restoring expression, delivered wild-type to pigmented...
Immunotherapies have shown promising results in certain cancer patients. For hepatocellular carcinoma (HCC), the multiplicity of an immunotolerant microenvironment within both tumor, and liver per se may limit efficacy immunotherapies. Since radiation induces immunogenic cell death inflammatory reactions tumor microenvironment, we hypothesized that a combination therapy lasting local immunostimulating agents, achieved by intratumoral injection adenoviral vector encoding interleukin 12,...
Migration of vascular smooth muscle cells (VSMCs) into the intima is considered to be a vital event in pathophysiology atherosclerosis. Despite substantial evidence supporting pathogenic role Toll-like receptor 4 (TLR4) progression atherogenesis, its function regulation VSMC migration remains unclear. The goal present study was elucidate mechanism by which TLR4 regulates migration. Inhibitor experiments revealed that TLR4-induced IL-6 secretion and were mediated via concerted actions MyD88...
A characteristic of diabetes mellitus is hyperglycemia, which considered with an emphasis on the diabetic retinopathy progressive neurodegenerative disease. Retinal ganglion cells (RGCs) are believed to be important affected in pathogenesis retinopathy. Transforming growth factor-beta (TGF-β) a neuroprotective protein that helps withstand various neuronal injuries. To investigate potential roles and regulatory mechanisms TGF-β hyperglycemia-triggered damage RGCs vitro, we established 5.5,...
Orthotopic allograft transplantation (OAT) is a significant approach to addressing organ failure. However, persistent immune responses the affect chronic rejection, which induces OAT vasculopathy (OATV) and Porphyromonas gingivalis can infiltrate remote organs via bloodstream, thereby intensifying severity of cardiovascular, respiratory, neurodegenerative diseases cancer. GroEL, virulence factor P. promotes pro-inflammatory cytokine production in host cells, assumes play pivotal role...
Yang H‐Y, Lin C‐Y, Tsai Y‐T, Lee C‐S. Experience of heart transplantation from hemodynamically unstable brain‐dead donors with extracorporeal support. Abstract: The shortage organ remains a major problem for worldwide. Potential after brain death may become unstable, despite maximal medical management, which ultimately leads to failure procurement. We reviewed the records five potential who presented hemodynamic instability management that were supported by circulation membrane oxygenation...
Atrial fibrillation is the most common complication of cardiac surgery and associated with significant morbidity mortality. Recognizing patients at high risk for developing postoperative atrial (POAF) may help identify those who could benefit from strategies to prevent POAF. This study was conducted delineate outcomes assess factors POAF among Taiwanese undergoing coronary artery bypass grafting (CABG).From January 2009 until February 2012, this prospective included 266 consecutive admitted...