In recent times, the counterfeiting of pharmaceuticals has been considered a serious trouble especially in developing countries that acquire poor inspection programs. Sildenafil, vardenafil and tadalafil (phosphodiesterase type 5 inhibitors) products have gained wide popularity treating sexual disorders, for which they are subjected to counterfeiting. For this purpose, simple, rapid, novel HPLC method with ultraviolet detection simply developed simultaneous determination vardenafil,...

Abstract Overexposure to food colorants above the allowed daily intake (ADI) level can provoke hyperactivity and other disturbed behaviors especially in children. Two new methods were developed separate five synthetic colorants, which Tartrazine (E102), Sunset Yellow (E110), Allura Red (E129), Carmoisine (E122), Brilliant Blue (E133). They are labeled on a large variety of commercial products Egyptian market without mentioning their definite concentrations. Therefore, there was real need...

Antipsychotics are widely used to treat various mental disorders. Combination therapies were approved by the FDA manic states. Quetiapine fumarate, aripiprazole, asenapine maleate, and chlorpromazine HCl frequently for treatment of these Green analytical chemistry is primarily concerned with reducing waste generated during sample preparation or analysis. solvents, such as ethanol, being in HPLC an alternative acetonitrile. To this purpose, two new chromatographic methods developed determine...

Three methods for the simultaneous determination of two multicomponent mixtures containing phenobarbitone and ephedrine hydrochloride with theophylline, postafene [mix 1]; or dihydroxypropyltheophylline chlorpheniramine maleate 2] were proposed. The HPLC method depended on use gradient mobile phase system consisting (A) acetonitrile (B)12 mM ammonium acetate; pH 4.5 cyanopropyl column at a flow rate 1.5 mL min−1. Quantitation was achieved UV detection 215 nm, based peak area. other...

In this paper, novel green HPLC and HPTLC chromatographic methods were developed for the concurrent determination of moxifloxacin, levofloxacin, gemifloxacin in bulk pharmaceutical products. The method was used on Thermo C18 (4.6 × 250 mm, 5 µm). By mixing ethanol 20 mM sodium dihydrogen phosphate dihydrate (pH 5) a ratio 25:75, v/v, mobile phase created using isocratic elution. flow rate 1 mLmin−1. studied antibiotics separated well within 9.5 min. coated aluminum sheets with Silica gel 60...

A rapid method for the quantification of levetiracetam (LV) in human urine using high-performance liquid chromatography combined with UV detection (HPLC–UV) was developed and validated linearity, repeatability, limit detection, quantitation. In addition, a electrospray ionization mass spectrometry described its identification urine. Chromatographic separation achieved on 250 × 4.6 mm (i.d.) Discovery® (5 µm particle size) reversed-phase C18 analytical column 0.1% formic acid...

In this study, a Quick, Easy, Cheap, Effective, Rugged and Safe method (QuEChERS) was optimised to extract 30 pesticides which are the most used in Egypt soil drainage water. Optimisation performed by Experimental Design expert 7.1 get better recoveries. The procedure combined with developed GC-MS for determination of these pesticides. Total analysis time 36 min 1 ml injection volume. limit quantification (LOQ) ranged between 4.59 ng/ml, 15.91 ng/ml all Most standard deviations were range...

A new probe based on the complex of 1,2 dihydro-2-oxoquinoloine-4-carboxylic acid (DOCA) as a ligand with Europium (III) ion was developed for quantitation Moxifloxacin HCl (Moxi.HCl) in pharmaceuticals and human plasma using luminescence method. The metal to ratio is 1:2 determined by Job plot. determination Moxi.HCl static quenching upon coordination Moxi.HCl. negative value ΔG proves that this reaction spontaneous. calibration curve constructed Stern–Volmer equation range 0.05–80 µg mL−1....

Titanium dioxide (TiO2) or E 171 is the naturally occurring oxide of titanium. It has been used as a colourant to improve and brighten appearance foods. Several toxicological studies have revealed that nanoparticles can cause inflammation, tissue necrosis, renal apoptosis, immune response. Moreover, International Organization for Cancer Research listed TiO2 "possibly carcinogenic humans''. Food labels do not provide quantitative data amount added even qualitative limited. In recent years,...

β-Lactam antibiotics are commonly prescribed with β-lactamase inhibitors to patients, for that it is necessary develop an optimized chromatographic method which determine them simultaneously in biological fluids.

Abstract One of the most applied procedures for determination trace analytes in complex matrices is QuEChERS (an acronym Quick, Easy, Cheap, Effective, Rugged, and Safe). include an extraction step followed by a dispersive solid-phase (dSPE) cleaning-up from matrix components. A challenging task extracting determining pesticides samples high fat such as milk samples. This challenge induced innovation new adsorbents clean-up Z-Sep Plus® EMR-Lipid® to enable removal fatty components without...

Implementing green analytical techniques has been one of the main objectives chemistry community for past two decades.The aim transitioning to is establish new methods environmentally benign instead traditional methods.According WHO, 20 million people worldwide are affected by schizophrenia, and 45 bipolar disorders.For that, in this work we develop a HPTLC method quantify simultaneously four antipsychotics chloropromazine HCl, asenapine maleate, quetiapine fumarate aripiprazole pure form...

Summary Two methods were presented for the simultaneous determination of two multicomponent mixtures containing pyridoxine hydrochloride (B6) and cyanocobalamine (B12) with benfotiamine (BN) [mix. 1], or thiamine nitrate (B1) diclofenac sodium (DC) 2]. The first high-performance liquid chromatographic (HPLC) method depends on use a cyanopropyl column at flow rate 1.5 mL min−1 mobile phase consisting acetonitrile-25 mM ammonium acetate, pH 3.7 (10:90, v/v), mix. 1 5.0 by gradient elution,...

An LC method was developed for the quantitative determination of pipoxolan hydrochloride (PPH) and its degradant. Forced degradation studies were performed on bulk sample using acid (1 M hydrochloric acid), alkaline (0.2 sodium hydroxide), oxidation (0.33% hydrogen peroxide), heat (70 degrees C), photolytic degradation. The chromatographic fine-tuned samples generated from forced studies. Good resolution between peaks corresponding to degradants analyte achieved a Discovery C18 column (25 cm...

Abstract In this study, a simple, fully validated and rapid reversed-phase HPLC with photodiode array detector method was developed for the simultaneous determination of 11 selected phenolic antioxidants over 33 min in personal care food samples containing extracts green apple, pomegranate (Ponica granatum) argan oil (Argania spinosa). The performed using NUCLEODUR C18 column 5 µm particle size 12.5 cm length. mobile phase prepared as follows, solution A: 1% aqueous acetic acid B:...