A5039479085- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Plant Gene Expression Analysis
- Plant Parasitism and Resistance
- Plant tissue culture and regeneration
China National Rice Research Institute
2021-2024
Rice spotted-leaf mutants are ideal materials to study the molecular mechanism underlying programmed cell death and disease resistance in plants. LOC_Os07g04820 has previously been identified as candidate gene responsible for phenotype rice Spotted-leaf 26 (Spl26) mutant. Here, we cloned validated that is locus controlling of Spl26 by reverse functional complementation CRISPR/Cas9-mediated knockout mutant allele. The recessive wild-type spl26 allele (Oryza sativa 26, Osspl26) highly...
Our study investigates the genetic mechanisms underlying spotted leaf phenotype in rice, focusing on spl43 mutant. This mutant is characterized by persistent reddish-brown spots from seedling stage to maturity, leading extensive necrosis. Using map-based cloning, we localized responsible locus a 330 Kb region chromosome 2. We identified LOC_Os02g56000, named OsRPT5A, as causative gene. A point mutation substituting valine for glutamic acid, was critical factor phenotype. Functional...
Rice spotted leaf mutants are helpful to investigate programmed cell death (PCD) and defense response pathways in plants. Using a map-based cloning strategy, we characterized novel rice mutation splHM143 that encodes 7-hydroxymethyl chlorophyll reductase (OsHCAR). The wild-type (WT) allele could rescue the mutant phenotype, as evidenced by complementation analysis. OsHCAR was constitutively expressed at all tissues tested its expression products localized chloroplasts. exhibited PCD...
Our study explores the genetic mechanisms underlying spotted leaf phenotype in rice, focusing on rpt5a mutant characterized by persistent reddish-brown spots from seedling stage to maturity, leading extensive necrosis. Through map-based cloning, we localized responsible locus a 330Kb region chromosome 2 and identified LOC_Os02g56000 (named OsRPT5A) as causative gene. A point mutation OsRPT5A, substituting valine for glutamic acid, was critical factor phenotype. Functional complementation...
Rice spotted-leaf mutants serve as valuable resources for studying plant programmed cell death (PCD) and disease resistance mechanisms, making them crucial research on in rice. Map-based cloning was used to identify clone the gene