A5042496626- Cellular Mechanics and Interactions
- Erythrocyte Function and Pathophysiology
- Cell Adhesion Molecules Research
- Cellular transport and secretion
- Genetic and Kidney Cyst Diseases
- 3D Printing in Biomedical Research
- Hippo pathway signaling and YAP/TAZ
- Pancreatic function and diabetes
- Advanced Materials and Mechanics
- Hedgehog Signaling Pathway Studies
- Ubiquitin and proteasome pathways
- Genetic Syndromes and Imprinting
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- Planarian Biology and Electrostimulation
- Microtubule and mitosis dynamics
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
Université Paris-Saclay
2017-2023
Northwestern University
2023
Institut Gustave Roussy
2016-2023
Inserm
2017-2023
Migrating cells often use focal adhesions in order to move. Focal are less prominent migrating three-dimensional (3D) as compared with 2D environments. We looked for alternative adhesion structures supporting cell migration. analyzed the dynamics of clathrin-coated pits a 3D environment collagen fibers. Both topological cues and local engagement integrins triggered accumulation on Clathrin/adaptor protein 2 (AP-2) lattices pinched fibers by adopting tube-like morphology regulated an...
Abstract Acetylation of the lysine 40 α-tubulin (K40) is a post-translational modification occurring in lumen microtubules (MTs) and controlled by acetyl-transferase αTAT1. How αTAT1 accesses acetylates there has been an open question. Here, we report that acetylation starts at ends MTs progressively spreads longitudinally from there. We observed marks vivo re-growing after Nocodazole block, acetylated segments growing length with time. Bias for extremities was even more pronounced when...
Extracellular vesicles are now considered as active contributors to melanoma progression through their capacity modify the tumor microenvironment and favor formation of a pre-metastatic niche. These prometastatic roles tumor-derived EVs would pass interaction with extracellular matrix (ECM) its remodeling, in turn providing substrate favoring persistent cell migration. Nevertheless, directly interact ECM components is still questionable. In this study, we use electron microscopy pull-down...
Xenopus embryos are covered with a complex epithelium containing numerous multiciliated cells (MCCs). During late-stage development, there is dramatic remodeling of the that involves complete loss MCCs. Cell extrusion well-characterized process for driving cell while maintaining epithelial barrier function. Normal typically unidirectional, whereas bidirectional often associated disease (e.g. cancer). We describe two distinct mechanisms MCC extrusion, basal driven by Notch signaling and an...
Adhesive clathrin-coated structures steer cell migration by accumulating on collagen fibers decorated with specific ligands.
Abstract Xenopus embryos are covered with a complex epithelium containing numerous multiciliated cells (MCCs). During late stage development there is dramatic remodeling of the that involves complete loss MCCs. Cell extrusion well-characterized process for driving cell while maintaining epithelial barrier function. Normal typically unidirectional whereas bidirectional often associated disease (e.g. cancer). We describe two distinct mechanisms MCC extrusion, basal driven by Notch signaling...
Tissue crowding represents a critical challenge to epithelial tissues, which often respond via the irreversible process of live cell extrusion. We report size reduction macropinocytosis as an alternative mechanism. Macropinocytosis is triggered by tissue mechanosensory signaling, leading substantial internalization apical membrane and driving in that remodels epithelium alleviate crowding. this mechanism regulates long-term organization developing response proliferation-induced but also...
Abstract The skin of Xenopus embryos contains numerous multiciliated cells (MCCs), which collectively generate a directed fluid flow across the epithelial surface essential for distributing overlaying mucous. MCCs develop into highly specialized to this flow, containing approximately 150 evenly spaced centrioles that give rise motile cilia. MCC-driven can be impaired when ciliary dysfunction occurs, resulting in primary dyskinesia (PCD) humans. Mutations large number genes (∼50) have been...