A5049554175- Skin and Cellular Biology Research
- Cellular Mechanics and Interactions
- Hair Growth and Disorders
- Cervical Cancer and HPV Research
- Wound Healing and Treatments
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Plant Reproductive Biology
- Molecular Biology Techniques and Applications
- Corneal Surgery and Treatments
- Wnt/β-catenin signaling in development and cancer
- Microtubule and mitosis dynamics
- Skin Protection and Aging
- 14-3-3 protein interactions
- Plant Virus Research Studies
- Neurogenetic and Muscular Disorders Research
- Plant Disease Resistance and Genetics
- Proteoglycans and glycosaminoglycans research
- Fibroblast Growth Factor Research
- Animal Virus Infections Studies
- RNA regulation and disease
- Plant Surface Properties and Treatments
University of Michigan
2019-2025
National Institutes of Health
2018
Center for Cancer Research
2018
National Institute of Allergy and Infectious Diseases
2018
Integration of human papillomavirus (HPV) genomes into cellular chromatin is common in HPV-associated cancers. random, and each site unique depending on how where the virus integrates. We recently showed that tandemly integrated HPV16 could result formation a super-enhancer-like element drives transcription viral oncogenes. Here, we characterize landscape genomic architecture this integration locus to elucidate mechanisms promoted de novo super-enhancer formation. Using next-generation...
The intermediate filament protein keratin 14 (K14) provides vital structural support in basal keratinocytes of epidermis. Recent studies evidenced a role for K14-dependent disulfide bonding the organization and dynamics IFs skin keratinocytes. Here we report that knock-in mice harboring cysteine-to-alanine substitution at Krt14's codon 373 (C373A) exhibit alterations disulfide-bonded K14 species barrier defect secondary to enhanced proliferation, faster transit time altered differentiation A...
Abstract Cell cycle progression in mammals is modulated by two ubiquitin ligase complexes, CRL4 and SCF, which facilitate degradation of chromatin substrates involved the regulation DNA replication. One member complex, WD-40 containing protein RepID (DCAF14/PHIP), selectively binds activates a group replication origins. Here we show that recruits complex to prior synthesis, thus playing crucial architectural role proper licensing chromosomes for In absence RepID, cells rely on alternative...
The type I intermediate filament proteins keratin 14 (K14) and 15 (K15) are common to all complex epithelia. K14 is highly expressed by progenitor keratinocytes, in which it provides essential mechanical integrity gates keratinocyte entry into differentiation sequestering YAP1, a transcriptional effector of Hippo signaling, the cytoplasm. K15 has long been used as marker hair bulge stem cells though its specific role skin epithelia unknown. Here we show that lack two biochemical...
A large group of keratin genes (n=54 in the human genome) code for intermediate filament (IF)-forming proteins and show differential regulation epithelial cells tissues. Keratin expression can be highly informative about type tissue, differentiation status constituent biological context (e.g. normal versus diseased settings). The foundational principles underlying use to gain insight tissues primarily originated pioneering studies conducted 1980s. recent emergence single cell transcriptomics...
Abstract Human papillomaviruses (HPVs) are frequently integrated in HPV‐associated cancers. HPV genomes can be three patterns: A single genome (type I), multiple, tandemly II), and interspersed with host DNA III). Analysis of the organization type II III integration sites is complicated by their repetitive nature, as sequences individual repeats difficult to distinguish from each other. This article presents a method for directly visualizing using molecular combing combined fluorescent situ...
Summary The type I intermediate filament (IF) keratin 14 (K14) provides vital structural support in basal keratinocytes of epidermis. Recent studies evidenced a role for K14-dependent disulfide bonding the organization and dynamics IFs skin keratinocytes. Here we report that knock-in mice harboring cysteine-to-alanine substitution at codon 373 (C373A) Krt14 exhibit alterations disulfide-bonded K14 species barrier defect secondary to enhanced proliferation, faster transit time altered...
Summary A large group of keratin genes (n=54 in the human genome) code for intermediate filament (IF)-forming proteins and show differential regulation epithelial cells tissues. Keratin expression can be highly informative about type tissue, differentiation status constituent cells, biological context (e.g., normal vs. diseased settings). The foundational principles underlying use to gain insight tissues primarily originated pioneering studies conducted 1980s. recent emergence single cell...