A5057359707- Retinal Development and Disorders
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Cellular Mechanics and Interactions
- Skin and Cellular Biology Research
- Nanoplatforms for cancer theranostics
- Corneal surgery and disorders
- Neuroscience and Neural Engineering
- Biotechnology and Related Fields
- Nerve injury and regeneration
- Corneal Surgery and Treatments
- Plant Surface Properties and Treatments
- Single-cell and spatial transcriptomics
- Aortic Disease and Treatment Approaches
- Neurogenesis and neuroplasticity mechanisms
- Plant Reproductive Biology
- 14-3-3 protein interactions
- Pluripotent Stem Cells Research
- Biomedical Ethics and Regulation
- RNA and protein synthesis mechanisms
- Cardiovascular and Diving-Related Complications
- RNA regulation and disease
- Melanoma and MAPK Pathways
- Hippo pathway signaling and YAP/TAZ
Johns Hopkins Medicine
2014-2020
Johns Hopkins University
2014-2020
Abstract Retinal ganglion cell (RGC) injury and death from glaucoma other forms of optic nerve disease is a major cause irreversible vision loss blindness. Human pluripotent stem (hPSC)-derived RGCs could provide source cells for the development novel therapeutic molecules as well potential cell-based therapies. In addition, such insights into human RGC development, gene regulation neuronal biology. Here, we report simple, adherent culture protocol differentiation hPSCs to using...
Significance Cell-based approaches utilizing retinal pigment epithelial (RPE)-like cells derived from human pluripotent stem (hPSCs) are being developed for the treatment of degeneration. In most research published to date, choice factors used induce RPE differentiation is based on data developmental studies. Here, we an unbiased approach directed at identifying novel differentiation-promoting using a high-throughput quantitative PCR screen complemented by orthogonal induced cell...
The intermediate filament protein keratin 14 (K14) provides vital structural support in basal keratinocytes of epidermis. Recent studies evidenced a role for K14-dependent disulfide bonding the organization and dynamics IFs skin keratinocytes. Here we report that knock-in mice harboring cysteine-to-alanine substitution at Krt14's codon 373 (C373A) exhibit alterations disulfide-bonded K14 species barrier defect secondary to enhanced proliferation, faster transit time altered differentiation A...
Axon injury is a hallmark of many neurodegenerative diseases, often resulting in neuronal cell death and functional impairment. Dual leucine zipper kinase (DLK) has emerged as key mediator this process. However, while DLK inhibition robustly protective wide range disease models, it also inhibits axonal regeneration. Indeed, there are no genetic perturbations that known to both improve long-term survival promote To identify such neuroprotective target, we conducted set complementary...
ABSTRACT CRISPR-Cas9 genome-editing is a revolutionary technology that transforming biological research. The explosive growth and advances in CRISPR research over the last few years, coupled with potential for clinical applications therapeutics, heralding new era genome engineering. To further support this platform to provide universal annotation system, we introduce grID database ( http://crispr.technology ), an extensive compilation of gRNA properties including sequence variations,...
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Summary The type I intermediate filament (IF) keratin 14 (K14) provides vital structural support in basal keratinocytes of epidermis. Recent studies evidenced a role for K14-dependent disulfide bonding the organization and dynamics IFs skin keratinocytes. Here we report that knock-in mice harboring cysteine-to-alanine substitution at codon 373 (C373A) Krt14 exhibit alterations disulfide-bonded K14 species barrier defect secondary to enhanced proliferation, faster transit time altered...