A5101966958- Retinal Development and Disorders
- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- CRISPR and Genetic Engineering
- Ocular Oncology and Treatments
- RNA Interference and Gene Delivery
- Genetics, Aging, and Longevity in Model Organisms
- RNA regulation and disease
- Neuroscience and Neural Engineering
- Invertebrate Immune Response Mechanisms
- interferon and immune responses
- Retinoids in leukemia and cellular processes
- Microbial Natural Products and Biosynthesis
- Melanoma and MAPK Pathways
- MicroRNA in disease regulation
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer, Lipids, and Metabolism
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Axon Guidance and Neuronal Signaling
- Corneal Surgery and Treatments
- Prostate Cancer Treatment and Research
- CAR-T cell therapy research
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
Johns Hopkins University
2008-2023
Johns Hopkins Medicine
2014-2023
University of Baltimore
2010
Although the presence of tumor-infiltrating lymphocytes (TILs) indicates an endogenous antitumor response, immune regulatory pathways can subvert effector phase and enable tumor escape. Negative include extrinsic suppression mechanisms, but also a T cell-intrinsic dysfunctional state. A more detailed study has been hampered by lack cell surface markers defining tumor-specific TILs, PD-1 alone is not sufficient. Recently, we identified transcription factor Egr2 as critical component in...
Introduction Oligodendrocytes (OLs) are the myelin-forming cells of central nervous system (CNS). Although OLs can be differentiated from human-induced pluripotent stem (hiPSCs), in vitro modeling axon myelination human remains challenging. Brain microphysiological systems (bMPS, e.g. organoids) complex three-dimensional (3D) cultures that offer an ideal to study this process as differentiate a more vivo -like environment; surrounded by neurons and astrocytes, which support axons. Methods...
Calsyntenins/alcadeins are type I transmembrane proteins with two extracellular cadherin domains highly expressed in mammalian brain. They form a tripartite complex X11/X11L and APP (amyloid precursor protein) proteolytically processed similar fashion to APP. Although genetic association of calsyntenin-2 human memory performance has recently been reported, physiological roles molecular functions the protein nervous system poorly understood. Here, we show that CASY-1, Caenorhabditis elegans...
Promoting myelination capacity of endogenous oligodendrocyte precursor cells (OPCs) is a promising therapeutic approach for CNS demyelinating disorders such as Multiple Sclerosis (MS). To aid in the discovery myelination-promoting compounds, we generated genome-engineered human pluripotent stem cell (hPSC) line that consists three reporters: identification-and-purification tag, GFP, and secreted-NanoLuc, driven by PDGFRA, PLP1, MBP genes, respectively. Using this line, established...
Epithelial-mesenchymal transition (EMT), which is well known for its role in embryonic development, malignant transformation, and tumor progression, has also been implicated a variety of retinal diseases, including proliferative vitreoretinopathy (PVR), age-related macular degeneration (AMD), diabetic retinopathy. EMT the pigment epithelium (RPE), although important pathogenesis these conditions, not understood at molecular level. We others have shown that molecules, co-treatment human stem...
Purpose: RPE injury often induces epithelial to mesenchymal transition (EMT). Although RPE-EMT has been implicated in a variety of retinal diseases, including proliferative vitroretinopathy, neovascular and atrophic AMD, diabetic retinopathy, it is not well-understood at the molecular level. To contribute our understanding EMT human RPE, we performed time-course transcriptomic analysis stem cell-derived (hRPE) monolayers induced undergo using 2 independent, yet complementary, model systems....
Pluripotent stem cells (PSCs) edited with genetic reporters are useful tools for differentiation analysis and isolation of specific cell populations study. Reporter integration into the genome is now commonly achieved by targeted DNA nuclease-enhanced homology directed repair (HDR). However, human PSCs known to have a low frequency gene knock-in (KI) HDR, making reporter line generation an arduous process. Here, we report methodology scarless KI large fluorescent genes transient selection...
ABSTRACT Promoting myelination capacity of endogenous oligodendrocyte precursor cells (OPCs) is a promising therapeutic approach for central nervous system demyelinating disorders such as Multiple Sclerosis (MS). To aid in the discovery promoting compounds, we generated an advanced, genome engineered, human pluripotent stem cell (hPSC) line that consist three reporters (identification-and-purification tag, GFP, and secreted NanoLuc) driven by PDGFRα , PLP1 MBP genes, respectively. Based upon...
ABSTRACT The critical role of epithelial to mesenchymal transition (EMT) in embryonic development, malignant transformation, and tumor progression has been well studied normal cancerous tissues cells. Interestingly, EMT also reported play a key the early several retinal degenerative diseases, including scarring associated proliferative vitro-retinopathy (PVR), choroidal neo-vascularization induced “wet” age-related macular degeneration (AMD) diabetic retinopathy (DR). Despite these studies,...
Abstract Pluripotent stem cells (PSCs) edited with genetic reporters are useful tools for differentiation analysis and isolation of specific cell populations study. Reporter integration into the genome is now commonly achieved by targeted DNA nuclease-enhanced homology directed repair (HDR). However, human PSCs known to have a low frequency gene knock-in (KI) HDR, making reporter line generation an arduous process. Here, we report methodology scarless KI large fluorescent genes transient...
e16081 Background: Though about half of newly diagnosed prostate cancer may not require immediate treatment, it remains the second most common cause death in United States indicating a need for novel preventive and therapeutic treatments. Here we use expression based analysis to identify “druggable” targets which were then used tailor screens commercially available candidate inhibitors cancer. Methods: A list 537 differentially expressed genes was developed by comparing profiles from men...
Abstract We have previously demonstrated a critical role for the mammalian Target Of Rapamycin (mTOR) in regulating T effector and regulatory cell differentiation. In light of fact that TOR is highly evolutionarily conserved, we hypothesized this signaling pathway would play Drosophila immune responses. Toll pathway, which characterized by induction drosomycin, responsible fighting fungal gram positive infections while deficiency (IMD) attacin induction, responds to negative infection. S2...