A5101888908- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Spinal Fractures and Fixation Techniques
- Epigenetics and DNA Methylation
- Insect and Arachnid Ecology and Behavior
- Insect and Pesticide Research
- Immune cells in cancer
- Atomic and Molecular Physics
- Laser-Matter Interactions and Applications
- Spine and Intervertebral Disc Pathology
- Advanced Chemical Physics Studies
- Plant and animal studies
- Insect-Plant Interactions and Control
- Animal Genetics and Reproduction
- Marine Biology and Ecology Research
- Hymenoptera taxonomy and phylogeny
- Spinal Cord Injury Research
- Biomedical Ethics and Regulation
- Trauma and Emergency Care Studies
- Insect behavior and control techniques
- Cinema and Media Studies
- T-cell and B-cell Immunology
- Genetically Modified Organisms Research
University of New Mexico
2013-2024
Brigham and Women's Hospital
2020-2022
Harvard University
2020-2022
University of Nevada, Reno
2021
University of Chicago
2013-2020
Williams (United States)
2013-2020
Creative Commons
2016-2017
Dalhousie University
2008-2017
Goethe University Frankfurt
2013-2016
University of Illinois Chicago
2015
T cell infiltration of solid tumors is associated with favorable patient outcomes, yet the mechanisms underlying variable immune responses between individuals are not well understood. One possible modulator could be intestinal microbiota. We compared melanoma growth in mice harboring distinct commensal microbiota and observed differences spontaneous antitumor immunity, which were eliminated upon cohousing or after fecal transfer. Sequencing 16S ribosomal RNA identified Bifidobacterium as...
Tumor escape from immune-mediated destruction has been associated with immunosuppressive mechanisms that inhibit T cell activation. Although evidence for an active immune response, including infiltration CD8(+) cells, can be found in a subset of patients, those tumors are nonetheless not immunologically rejected. In the current report, we show it is cell-inflamed showed high expression three defined mechanisms: indoleamine-2,3-dioxygenase (IDO), PD-L1/B7-H1, and FoxP3(+) regulatory cells...
Although the presence of tumor-infiltrating lymphocytes (TILs) indicates an endogenous antitumor response, immune regulatory pathways can subvert effector phase and enable tumor escape. Negative include extrinsic suppression mechanisms, but also a T cell-intrinsic dysfunctional state. A more detailed study has been hampered by lack cell surface markers defining tumor-specific TILs, PD-1 alone is not sufficient. Recently, we identified transcription factor Egr2 as critical component in...
Abstract Subsets of human tumors are infiltrated with tumor antigen–specific CD8+ T cells [tumor-infiltrating lymphocytes (TILs)] despite progression. These TILs thought to be inactivated by the immunosuppressive microenvironment, through engagement inhibitory receptors such as CTLA-4 and PD-1. However, antigen-specific not functionally inert but undergoing activation in situ. Here, we show that actively proliferating, yet also undergo high rates apoptosis, leading a vicious cycle death...
PD-1/PD-L1 blockade can promote robust tumor regression yet secondary resistance often occurs as immune selective pressure drives outgrowth of resistant clones. Here using a genome-wide CRISPR screen in B16.SIY melanoma cells, we confirm Ifngr2 and Jak1 important genes conferring sensitivity to T cell-mediated killing vitro. However, when implanted into mice, these Ifngr2- Jak1-deficient tumors paradoxically are better controlled immunologically. This phenotype maps defective PD-L1...
A critical but seldom-studied component of life history theory is how behavior and age affect whole-organism performance. To address this issue we compared the flight performance honey bees (whose behavioral development can be assessed independently via simple manipulations colony demographics) between distinct castes (in-hive nurse vs out-of-hive foragers) across lifespan. Variable-density gases high-speed video were used to determine maximum hovering capacity wing kinematics age-matched...
Healthcare-acquired infections with methicillin-resistant Staphylococcus aureus (MRSA) are a significant cause of increased mortality, morbidity and additional health care costs in United States. Surface decontamination technologies that utilize pulsed xenon ultraviolet light (PPX-UV) may be effective at reducing microbial burden. The purpose this study was to compare standard manual room-cleaning PPX-UV disinfection technology for MRSA bacterial heterotrophic plate counts (HPC) on...
During the past 15 years a novel decay mechanism of excited atoms has been discovered and investigated. This so-called interatomic Coulombic (ICD) involves chemical environment electronically atom: excitation energy is transferred (in many cases over long distances) to neighbor initially particle usually ionizing that neighbor. It turned out ICD very common route in nature as it occurs across van der Waals hydrogen bonds. The time evolution predicted be highly complex, its efficiency...
The objectives of this study are to examine hepatic gene expression changes caused by GH transgenesis and enhanced growth. This is the first use cDNA microarrays influence on liver in a non-mammalian vertebrate, such using sexually immature animals. Three groups coho salmon were examined: transgenic full ration (T), restricted (R), control non-transgenic (C). Specific growth rates for weight T approximately eightfold higher than C, fourfold R. Differential T, R, C samples was determined...
The Wilderness Medical Society reconvened an expert panel to update best practice guidelines for spinal cord protection during trauma management. This panel, with membership updated in 2023, was charged the development of evidence-based management injured or potentially spine wilderness environments. Recommendations are made regarding several parameters related protection. These recommendations graded based on quality supporting evidence and balance benefits risks/burdens each parameter...
Abstract Prepupae of the rose galling Diplolepis spinosa from areas with relatively cold winters in southern Canada, and variabilis a milder locale western were used to test hypothesis that mild winter temperatures are detrimental survival potential fecundity insects. D. held within or removed their galls exposed simulated overwintering (−22, 0, 5, 10°C) for approximately four months before measuring survival, body size, fecundity. Similar studies conducted using prepupae gall subjected 0°C...
An oligonucleotide microarray containing 50‐mer oligonucleotides representing 9277 unique Atlantic halibut Hippoglossus hippoglossus genes has been designed, printed and is currently being used for the study of gene expression in developing halibut. The are based on all data available at time printing, these included expressed sequence tags (ESTs) complete cDNAs derived from Pleurogene sequencing project as well sequences deposited GenBank by other groups September 2006. Of ESTs, 5040...
T-cell exhaustion in cancer is linked to poor clinical outcomes, where evidence suggests metabolic changes precede functional exhaustion. Direct competition between tumor-infiltrating lymphocytes (TIL) and cells for resources often renders T dysfunctional. Environmental stress produces epigenome remodeling events within TIL resulting from loss of the histone methyltransferase EZH2. Here, we report an epigenetic mechanism contributing development TIL. A multiomics approach revealed a...
Abstract Monoclonal antibodies (abs) targeting the programmed cell death 1 (PD-1) immune checkpoint pathway have revolutionized tumor therapy. Because T-cell-directed PD-1 blockade boosts immunity, anti-PD-1 abs been developed for examining T-cell-PD-1 functions. More recently, expression has also reported directly on cancer cells of various etiology, including in melanoma. Nevertheless, there is a paucity studies validating ab clone utility specific assay types characterizing cell-intrinsic...
The present study was conducted to follow up on apparent differences in growth, relative organ sizes, cellular stress and immune function Atlantic salmon fed feed containing GM Bacillus thuringiensis maize compared with the non-modified parental line. Gene expression profiling distal intestinal segment liver performed by microarray, selected genes were followed quantitative PCR (qPCR). In liver, qPCR revealed some differentially regulated genes, including up-regulation of gelsolin precursor,...
Possible links between seasonal increases in cold-tolerance and desiccation resistance were examined field-collected larvae of the goldenrod gall fly, Eurosta solidaginis. From 20 September to 30 October 2001, exhibited a gradual increase culminating 100% survival freezing at -20 degrees C for 24 h. The was probably due concomitant cryoprotectants as measured by hemolymph osmolality (488-695 mOsmol kg(-1)). In contrast cold-tolerance, two distinct phases reduced rates water loss. first phase...
We investigate the dissociation of H_{2}^{+} into a proton and H^{0} after single ionization with photons an energy close to threshold. find that p^{+} do not emerge symmetrically in case dissociating along 1sσ_{g} ground state. Instead, preference for ejection direction escaping photoelectron can be observed. This symmetry breaking is strongest very small electron energies. Our experiment consistent recent prediction by Serov Kheifets [Phys. Rev. A 89, 031402 (2014)]. In their model, which...
Four species of cynipid wasp the genus Diplolepis that induce galls on roses (Rosa species) in southern Canada and two inquiline associated with these were studied for their cold-hardiness resistance to water loss possible links between adaptations. Mid-winter-acclimated supranivean D. spinosa Periclistus pirata had lower supercooling points (-38 -40 degrees C) higher hemolymph osmolalities (1760-1849 mosmol kg(-1)) than subnivean polita, gracilis, radicum sp. (-31 -32 C 977-1464 kg(-1),...