A5012347503- Glaucoma and retinal disorders
- Retinal Development and Disorders
- Immune Cell Function and Interaction
- HIV Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Retinal Diseases and Treatments
- RNA Interference and Gene Delivery
- Cytomegalovirus and herpesvirus research
- Viral Infections and Outbreaks Research
- Hepatitis B Virus Studies
- CAR-T cell therapy research
- Immune Response and Inflammation
- Retinal Imaging and Analysis
- interferon and immune responses
- HIV/AIDS drug development and treatment
- Viral Infections and Vectors
- CRISPR and Genetic Engineering
- Retinopathy of Prematurity Studies
- Herpesvirus Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
- S100 Proteins and Annexins
- Viral gastroenteritis research and epidemiology
China Medical University
2017-2024
Sanofi (United States)
2017-2023
Frequentis (Germany)
2022
National Institutes of Health
2000-2022
National Institute of Allergy and Infectious Diseases
2011-2022
Institut de Recherche Vaccinale
2022
OPKO Health (United States)
2022
University of California, San Diego
2014-2020
AVEO Oncology (United States)
2020
Sanofi (France)
2020
Direct gene transfer offers the potential to introduce DNA encoding therapeutic proteins treat human disease. Previously, in humans has been achieved by a cell-mediated ex vivo approach which cells from blood or tissue of patients are genetically modified laboratory and subsequently returned patient. To determine feasibility safety directly transferring genes into humans, clinical study was performed. The foreign major histocompatibility complex protein, HLA-B7, introduced HLA-B7-negative...
Broadly neutralizing antibodies to HIV with similar specificities can be found in multiple HIV-infected individuals.
The arterial wall responds to thrombosis or mechanical injury through the induction of specific gene products that increase cellular proliferation and connective tissue formation. These changes result in intimal hyperplasia is observed restenosis early phases atherosclerosis. Transforming growth factor beta 1 (TGF-beta 1) a secreted multi-functional protein plays an important role embryonal development repair following injury. However, function TGF-beta vascular cell vivo has not been...
Over the past 5 years, a new generation of highly potent and broadly neutralizing HIV-1 antibodies has been identified. These can protect against lentiviral infection in nonhuman primates (NHPs), suggesting that passive antibody transfer would prevent transmission humans. To increase protective efficacy such monoclonal antibodies, we employed next-generation sequencing, computational bioinformatics, structure-guided design to enhance neutralization potency breadth VRC01, an targets CD4...
The development of an effective AIDS vaccine has been challenging because viral genetic diversity and the difficulty generating broadly neutralizing antibodies (bnAbs). We engineered trispecific (Abs) that allow a single molecule to interact with three independent HIV-1 envelope determinants: CD4 binding site, membrane-proximal external region (MPER), V1V2 glycan site. Trispecific Abs exhibited higher potency breadth than any previously described bnAb, showed pharmacokinetics similar those...
Glaucoma, a major cause of blindness worldwide, is neurodegenerative optic neuropathy in which vision loss caused by retinal ganglion cells (RGCs). To better define the pathways mediating RGC death and identify targets for development neuroprotective drugs, we developed high-throughput RNA interference screen with primary RGCs used it to full mouse kinome. The identified dual leucine zipper kinase (DLK) as key target RGCs. In cultured RGCs, DLK signaling both necessary sufficient cell death....
The immune system confers protection against a variety of pathogens and contributes to the surveillance destruction neoplastic cells. Several cell types participate in recognition lysis tumors, appropriate stimulation provides therapeutic effects malignancy. Foreign major histocompatibility complex (MHC) proteins also serve as potent stimulus system. In this report, foreign MHC gene was introduced directly into malignant tumors vivo an effort stimulate tumor rejection. contrast previous...
Arterial injury induces a series of proliferative, vasoactive, and inflammatory responses that lead to vascular proliferative diseases, including atherosclerosis restenosis. Although several factors have been defined which stimulate this process in vivo, the role specific cellular gene products limiting response is not well understood. The p21 cyclin-dependent kinase inhibitor affects cell cycle progression, senescence, differentiation transformed cells, but its expression injured blood...
Telomerase, a specialized RNA-directed DNA polymerase that extends telomeres of eukaryotic chromosomes, is repressed in human somatic tissues and becomes activated during tumor progression most cancers. To date, little known about how telomerase controlled cancer, although activation thought to be involved cancer cell immortalization. Here, we report telomerase-associated protein 1 (hTEP1) the catalytic subunit (human reverse transcriptase (hTERT)) are phosphoproteins their phosphorylation...
The fate of plasmid DNA complexed with cationic lipids delivered intravenously in mice was evaluated at selected timepoints up to 6 months postinjection. Blood half-life and tissue distribution potential expression tissues were examined. Southern blot analyses blood indicated that intact rapidly degraded, a less than 5 min for plasmid, no longer detectable 1 hr demonstrated differentially retained the lung, spleen, liver, heart, kidney, marrow, muscle 24 After 7 days, by analysis; however,...
Optic nerve head (ONH) astrocytes have been proposed to play both protective and deleterious roles in glaucoma. We now show that, within the postlaminar ONH myelination transition zone (MTZ), there are that normally express Mac-2 (also known as Lgals3 or galectin-3), a gene typically expressed only phagocytic cells. Surprisingly, even healthy mice, MTZ other constitutive internalize large axonal evulsions contain whole organelles. In mouse glaucoma models, further up-regulate expression....
Ebola virus causes a hemorrhagic fever syndrome that is associated with high mortality in humans. In the absence of effective therapies for infection, development vaccine becomes an important strategy to contain outbreaks. Immunization DNA and/or replication-defective adenoviral vectors (rAd) encoding glycoprotein (GP) and nucleoprotein (NP) has been previously shown confer specific protective immunity nonhuman primates. GP can exert cytopathic effects on transfected cells vitro, multiple...
The inflammatory response that accompanies central nervous system (CNS) injury can affect neurological outcome in both positive and negative ways. In the optic nerve, a CNS pathway normally fails to regenerate when damaged, intraocular inflammation causes retinal ganglion cells (RGCs) switch into an active growth state extend lengthy axons down nerve. molecular basis of this phenomenon is uncertain. A prior study showed oncomodulin (Ocm), Ca(2+)-binding protein secreted by macrophage cell...
purpose. The authors studied retinal gene expression changes in rats after experimental intraocular pressure elevation and optic nerve transection to elucidate molecular mechanisms of ganglion cell (RGC) death. methods. Translimbal laser photocoagulation was used induce unilateral IOP 41 albino Wistar rats. In 38 additional animals, the performed. Retinas were harvested 1 day, 3 days, week, 2 weeks, 4 8 weeks each treatment, total RNA isolated. Pooled from time point analyzed with rat genome...