A5086012376- Skin and Cellular Biology Research
- Reproductive Physiology in Livestock
- Heat shock proteins research
- Effects of Environmental Stressors on Livestock
- Bee Products Chemical Analysis
- Pancreatic function and diabetes
- Adipokines, Inflammation, and Metabolic Diseases
- Blood properties and coagulation
- Wnt/β-catenin signaling in development and cancer
- Adipose Tissue and Metabolism
- Toxin Mechanisms and Immunotoxins
- Fatty Acid Research and Health
- Plant Gene Expression Analysis
- Fungal Biology and Applications
- Animal health and immunology
- Biochemical effects in animals
- Genetic and phenotypic traits in livestock
- Milk Quality and Mastitis in Dairy Cows
- RNA Research and Splicing
- Peroxisome Proliferator-Activated Receptors
- Phytoestrogen effects and research
- Animal Behavior and Welfare Studies
- Ubiquitin and proteasome pathways
- Cancer-related molecular mechanisms research
- Plant Surface Properties and Treatments
Université de Sherbrooke
2012-2025
New York University Press
2017
Cambridge University Press
2017
Université de Montréal
2012
Agriculture and Agri-Food Canada
2012
Hôtel-Dieu de Québec
1989-1992
Université Laval
1989-1992
Baylor College of Medicine
1992
Glaucoma, a major cause of blindness worldwide, is neurodegenerative optic neuropathy in which vision loss caused by retinal ganglion cells (RGCs). To better define the pathways mediating RGC death and identify targets for development neuroprotective drugs, we developed high-throughput RNA interference screen with primary RGCs used it to full mouse kinome. The identified dual leucine zipper kinase (DLK) as key target RGCs. In cultured RGCs, DLK signaling both necessary sufficient cell death....
We have reported previously that the addition of dexamethasone to cultured quiescent suckling rat hepatocytes in presence insulin, a culture condition which does not cause growth activation, induces selective increase synthesis 49-kD/55-kD cytokeratin (CK49/CK55) pair over 24-h period. This increased coincides with formation dense filament networks reminiscent those observed situ at cell periphery (Marceau, N., H. Baribault, and I. Leroux-Nicollet. 1985. Can. J. Biochem. Cell Biol....
Dual leucine zipper kinase (DLK) is critical for neurite outgrowth in the developing nervous system and during nerve regeneration, but underlying mechanisms remain largely unknown. To address this issue, we generated stable shRNA-mediated DLK-depleted Neuro-2a cell lines analyzed their phosphoproteome after induction of neuronal differentiation by retinoic acid (RA). Here, report identification 32 phosphopeptides that exhibited significant differences relative abundance between control...
In the skin, epithelial cells undergo a terminal differentiation program leading to formation of stratum corneum. Although it is expected that last phases this process must be tightly regulated since results in cell death, signaling pathways involved induction remain ill defined. We now report single kinase, mitogen-activated protein kinase dual leucine zipper-bearing (DLK), acts epidermis promote human keratinocytes. support notion, we showed DLK expression was restricted granular layer...
Although beneficial effects have been attributed to PUFA supplementation in high-yielding dairy cows, diets rich may also increase oxidative stress tissues such as the liver. To fully exploit health benefits of PUFA, we believe that addition natural antioxidants could help preventing damage. Using an vitro precision-cut liver slices (PCLS) tissue culture system, investigated different linoleic acid (LA, n-6):α-linolenic (ALA, n-3) ratios (LA:ALA ratio 4, LA:ALA 15 and 25) presence or absence...
The mixed-lineage kinase (MLK) family member DLK has been proposed to serve as a regulator of differentiation in various cell types; however, its role adipogenesis not investigated. In this study, we used the 3T3-L1 preadipocyte line model examine function adipocyte differentiation.Immunoblot analyses and assays performed on cells showed that expression activity substantially increase occurs. Interestingly, appears crucial for since depletion by RNA interference impairs lipid accumulation...
Adipogenesis, the biological process by which preadipocytes differentiate into mature fat cells, is coordinated a tightly regulated gene expression program. Indeed, it has been reported that large number of genetic events, from cell-specific transcription factors expression, such as master regulator cell differentiation peroxisome proliferator-activated receptor (PPAR)γ2 to epigenetic modifications, govern acquisition adipocyte phenotype. Here, we provide evidence E1A-binding protein p400...
Current evidence suggests that the mixed lineage kinase family member dual leucine zipper-bearing (DLK) might play a significant role in regulation of cell growth and differentiation, particularly during process tissue remodeling. To further explore this working model, we have investigated host recombinant DLK NIH3T3 COS-1 cells undergoing apoptosis. Using calphostin C, potent selective inhibitor protein C recognized apoptosis inducer for various types, demonstrate, by immunoblot analysis,...
During the transition period, dairy cows often experience negative energy balance, which induces metabolic and immunological disturbances. Our previous work has shown a relationship between inhibition of immune functions increased blood nonesterified fatty acid (NEFA) levels. In this study, we evaluated effect 11 acids (palmitoleic, myristic, palmitic, stearic, oleic, linoleic, docosahexaenoic, conjugated lauric, eicosapentaenoic, linolenic acids) as well mix that represented NEFA profile...
Mammary gland secretory activity is modulated by systemic and local factors; however, the relationship between these factors unknown. The aim of this study was to determine how a factor, such as incomplete milking, affects mammary epithelial cell activity, number, responsiveness blood prolactin (PRL). Eight cows in mid-lactation were differentially milked (i.e., their right quarters incompletely at approximately 70%, left completely, twice daily for 4 wk). Throughout experiment, milk yield...
During the transition period, dairy cows often experience negative energy balance, which can induce metabolic and immunological disturbances. Previous work has shown that there is a relationship between dysfunction of immune cells increase in blood nonesterified fatty acid (NEFA) concentration. Nevertheless, it difficult to determine exact effect NEFA on system, as other hormonal perturbations occur simultaneously during period. In present study, we have determined functions using an...
Cows undergoing a negative energy balance (NEB) often experience state of immunosuppression and are at greater risk infectious diseases. The present study aimed to evaluate the impact folic acid vitamin B12 supplement feed restriction on several immune parameters. Sixteen cows 45 ± 3 days in milk were assigned 8 blocks 2 each according cow's production previous week, within block, randomly received weekly intramuscular injections either saline or 320 mg 10 for 5 weeks. During week 5, fed 75%...
ZPK is a recently described protein serine/threonine kinase that has been originally identified from human teratocarcinoma cell line by the polymerase chain reaction and whose function in signal transduction not yet elucidated. To investigate potential role of this developmental processes, we have analyzed spatial temporal patterns expression gene mouse embryos different gestational ages. Northern blot analysis revealed single mRNA species about 3.5 KB Day 11 gestation onwards. In situ...
ZPK is a recently identified human putative protein kinase gene that encodes an unusual serine/threonine containing two potential leucine zipper motifs similar to those found in transcription factors as well members of the newly discovered mixed-lineage family kinases. To study normal biological function ZPK, we have isolated mouse cDNA and examined pattern mRNA expression adult tissues by Northern blot situ hybridization analyses. The predicted open reading frame this 888-amino-acid shares...