A5069279560- Retinal Development and Disorders
- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Nerve injury and regeneration
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Circadian rhythm and melatonin
- Neuroscience and Neural Engineering
- melanin and skin pigmentation
- Retinal and Optic Conditions
- Neurological Disorders and Treatments
- Retinoids in leukemia and cellular processes
- Traumatic Brain Injury and Neurovascular Disturbances
- Retinopathy of Prematurity Studies
- Axon Guidance and Neuronal Signaling
- Mesenchymal stem cell research
- Ocular Surface and Contact Lens
- Ocular and Laser Science Research
- Aldose Reductase and Taurine
- Corneal Surgery and Treatments
- Neurological Disease Mechanisms and Treatments
- Cerebral Venous Sinus Thrombosis
- Cell death mechanisms and regulation
Universidad de Murcia
2016-2025
Instituto Murciano de Investigación Biosanitaria
2016-2025
Centro de Investigacion Principe Felipe
2021
Mineral Products Association
2019
Université Jean Monnet
2018
Biologie, ingénierie et imagerie pour l'Ophtalmologie
2018
Hospital Universitario Virgen de la Arrixaca
2005-2016
University of Coimbra
2016
Montreal General Hospital
1985-2015
McGill University
1985-2015
In adult rats, one optic nerve was transected and replaced by a 4 cm segment of autologous peripheral (PN) that linked eye the superior colliculus (SC) along predominantly extracranial course. Retrograde orthograde studies with tracers HRP or rhodamine-B-isothiocyanate (RITC), as well immunocytochemical neuronal labels, indicated following: (1) Regenerating axons from axotomized retinal ganglion cells extended entire PN grafts, covering distance nearly twice normal retinotectal projection...
To characterize Brn3a expression in adult albino rat retinal ganglion cells (RGCs) naïve animals and subjected to complete intraorbital optic nerve transection (IONT) or crush (IONC).Rats were divided into three groups, naïve, IONT, IONC. Two-, 5-, 9-, 14-day postlesion (dpl) retinas examined for immunoreactivity Brn3a. Before the injury, RGCs labeled with Fluorogold (FG; Fluorochrome, Corp. Denver, CO). was also determined by Western blot analysis. The proportion of double FG radial...
Abstract To investigate the short‐and long‐term effects of axotomy on survival central nervous system (CNS) neurons in adult rats, retinal ganglion cells (RGCs) were labelled retrogradely with persistent market diI and their axons interrupted optic nerve (ON) by intracranial crush 8 or 10 mm from eye intraorbital cut 0.5 3 eye. Labelled RGCs counted flat‐mounted retinas at intervals 2 weeks to 20 months after axotomy. Two major patterns RGC loss observed: (1) an inital abrupt that was...
To investigate the role of extrinsic influences on survival and growth axotomized retinal ganglion cells (RGCs) in mature mammalian CNS, both optic nerves (ONs) adult rats were transected intraorbitally and, one side, replaced by an autologous segment peripheral nerve (PN) that had been left unconnected distally. The RGCs regrowth their cut axons into PN grafts assessed using morphometric techniques, neuroanatomic tracers, immunologic cell markers to identify count at times ranging from 15 d...
Ocular hypertension is a major risk factor for glaucoma, neurodegenerative disease characterized by an irreversible decrease in ganglion cells and their axons. Macroglial microglial appear to play important role the pathogenic mechanisms of disease. Here, we study effects laser-induced ocular (OHT) macroglia, microglia retinal (RGCs) eyes with OHT (OHT-eyes) contralateral two weeks after lasering.Two groups adult Swiss mice were used: age-matched control (naïve, n=9); lasered (n=9). In...
Autologous peripheral nerve grafts were used to permit and direct the regrowth of retinal ganglion cell axons from eye ipsilateral superior colliculus adult hamsters in which optic nerves had been transected within orbit. Extracellular recordings 15 18 weeks after graft insertion revealed excitatory inhibitory postsynaptic responses visual stimulation. The finding light-induced neurons superficial layers close indicates that regenerating axotomized cells can establish electrophysiologically...
Optic nerve head (ONH) astrocytes have been proposed to play both protective and deleterious roles in glaucoma. We now show that, within the postlaminar ONH myelination transition zone (MTZ), there are that normally express Mac-2 (also known as Lgals3 or galectin-3), a gene typically expressed only phagocytic cells. Surprisingly, even healthy mice, MTZ other constitutive internalize large axonal evulsions contain whole organelles. In mouse glaucoma models, further up-regulate expression....
Glaucomatous optic neuropathy, a leading cause of blindness, can progress despite control intraocular pressure - currently the main risk factor and target for treatment. Glaucoma progression shares mechanisms with neurodegenerative disease, including microglia activation. In present model ocular hypertension (OHT), we have recently described morphological signs retinal activation MHC-II upregulation in both untreated contralateral eyes OHT eyes. By using immunostaining, sought to analyze...
The three members of the Pou4f family transcription factors: Pou4f1, Pou4f2, Pou4f3 (Brn3a, Brn3b and Brn3c, respectively) play, during development, essential roles in differentiation survival sensory neurons. purpose this work is to study expression Brn3 factors albino pigmented adult rat. Animals were divided into these groups: i) untouched; ii) fluorogold (FG) tracing from both superior colliculli; iii) FG-tracing one colliculus; iv) intraorbital optic nerve transection or crush. All...
Purpose: To investigate retinal ganglion cell (RGC) survival and activation of caspase 3 after optic nerve crush (ONC) or transection (ONT) treatment with brain-derived neurotrophic factor (BDNF) Z-DEVD_fmk. Methods: In albino Swiss mice, the left was severed crushed at 0.5 mm from head retinas were analyzed 1 to 10 days. Additional groups treated intravitreally a single injection BDNF (2.5 μg) Z-DEVD_fmk (125 ng) right injury, day 2, multiple injections As controls intact vehicle-treated...
We purpose here to analyze and compare the population topography of cone photoreceptors in two mouse strains using automated routines, design a method retinal sampling for their accurate manual quantification. In whole-mounted retinas from pigmented C57/BL6 albino Swiss mice, longwave-sensitive (L) shortwave-sensitive (S) opsins were immunodetected each type. another group both detected with same fluorophore quantify all cones. third set retinas, L-opsin Brn3a determine whether L-opsin+cones...
To assess the effect of a single intravitreal injection brain-derived neurotrophic factor (BDNF) on survival mouse retinal ganglion cells (RGCs) and phagocytic microglia after intraorbital optic nerve transection (IONT).One week before IONT or processing, RGCs from pigmented C57/BL6 albino Swiss mice were traced by applying hydroxystilbamidine methanesulfonate (OHSt) to both superior colliculi. Right afterward unilateral IONT, BDNF vehicle intravitreally administered. At increasing time...
Purpose: To analyze the long-term effect of optic nerve injury on retinal ganglion cells (RGCs) and melanopsin+RGCs orthotopic displaced, rest cell layer (GCL) cells. Methods: In adult albino rats, left was crushed (ONC) or transected (ONT). Injured contralateral retinas were analyzed at increasing survival intervals (up to 15 months). study all GCL RGCs, immunodetected with Brn3a melanopsin identify general RGC population (Brn3a+) m+RGCs, counter-stained 4′,6-diamidino-2-phenylindole...