A5049941713- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Retinal Imaging and Analysis
- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Neurological Disorders and Treatments
- Cell Adhesion Molecules Research
- Neuroscience and Neuropharmacology Research
- Amyotrophic Lateral Sclerosis Research
- Neurological Disease Mechanisms and Treatments
- Biochemical effects in animals
- Saffron Plant Research Studies
- Cerebral Venous Sinus Thrombosis
- Retinopathy of Prematurity Studies
- Barrier Structure and Function Studies
- Connexins and lens biology
- Cinema History and Criticism
- Corneal Surgery and Treatments
- Retinal and Optic Conditions
- Ophthalmology and Eye Disorders
- Laser Applications in Dentistry and Medicine
- Ocular and Laser Science Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Mitochondrial Function and Pathology
Universidad Complutense de Madrid
2016-2025
Instituto de Investigación Sanitaria del Hospital Clínico San Carlos
2021-2025
Lawrence Livermore National Laboratory
2024
Instituto de Óptica "Daza de Valdés"
2023
Instituto de Salud Carlos III
2017-2022
Benemérita Universidad Autónoma de Puebla
2022
Instituto Ramón y Cajal de Investigación Sanitaria
1997-2022
Hospital Clínico San Carlos
1990-2022
Comunidad de Madrid
2021
Hospital General Universitario Gregorio Marañón
2021
Ocular hypertension is a major risk factor for glaucoma, neurodegenerative disease characterized by an irreversible decrease in ganglion cells and their axons. Macroglial microglial appear to play important role the pathogenic mechanisms of disease. Here, we study effects laser-induced ocular (OHT) macroglia, microglia retinal (RGCs) eyes with OHT (OHT-eyes) contralateral two weeks after lasering.Two groups adult Swiss mice were used: age-matched control (naïve, n=9); lasered (n=9). In...
Glaucoma is a neurodegenerative disease of the retina characterized by irreversible loss retinal ganglion cells (RGCs) leading to visual loss. Degeneration RGCs and their axons, as well damage remodeling lamina cribrosa are main events in pathogenesis glaucoma. Different molecular pathways involved RGC death, which triggered exacerbated consequence number risk factors such elevated intraocular pressure (IOP), age, ocular biomechanics, or low perfusion pressure. Increased IOP one most...
Glaucomatous optic neuropathy, a leading cause of blindness, can progress despite control intraocular pressure - currently the main risk factor and target for treatment. Glaucoma progression shares mechanisms with neurodegenerative disease, including microglia activation. In present model ocular hypertension (OHT), we have recently described morphological signs retinal activation MHC-II upregulation in both untreated contralateral eyes OHT eyes. By using immunostaining, sought to analyze...
Alzheimer's Disease (AD) can cause degeneration in the retina and optic nerve either directly, as a result of amyloid beta deposits, or secondarily, degradation visual cortex. These effects raise possibility that tracking ophthalmologic changes be used to assess neurodegeneration AD. This study aimed detect retinal associated functional three groups patients consisting AD with mild disease, moderate disease healthy controls by using non-invasive psychophysical ophthalmological tests optical...
In the mouse model of unilateral laser-induced ocular hypertension (OHT) microglia in both treated and normotensive untreated contralateral eye have morphological signs activation up-regulation MHC-II expression comparison with naïve. brain, rod-like align to less-injured neurons an effort limit damage. We investigate whether: i) microglial is secondary laser injury or a higher IOP and; ii) presence related OHT. Three groups mice were used: age-matched control (naïve, n=15); two lasered:...
Decreased thickness of the retinal nerve fiber layer (RNFL) may reflect neuronal-ganglion cell death. A decrease in RNFL has been demonstrated Alzheimer’s disease (AD) addition to aging by optical coherence tomography (OCT). Twenty-three mild-AD patients and 28 age-matched control subjects with mean Mini-Mental State Examination 23.3 28.2, respectively, no ocular or systemic disorders affecting vision, were considered for study. OCT peripapillary macular segmentation examined right eye each...
Glaucoma is a neurodegenerative disease characterized by the loss of retinal ganglion cells (RGCs). An increase in intraocular pressure principal risk factor for such loss, but controlling this does not always prevent glaucomatous damage. Activation immune resident retina (microglia) may contribute to RGC death. Thus, substance with anti-inflammatory activity protect against degeneration. This study investigated neuroprotective and effects hydrophilic saffron extract standardized 3% crocin...
Abstract Microglial activation is associated with glaucoma. In the model of unilateral laser-induced ocular hypertension (OHT), time point at which inflammatory process peaks remains unknown. Different points (1, 3, 5, 8, and 15 d) were compared to analyze signs microglial both in OHT contralateral eyes. eyes, was detected all retinal layers analyzed, including: i) increase cell number outer segment photoreceptor layer plexiform (only eyes) from 3 d onward; ii) soma size 1 iii) retraction...
The murine models of Alzheimer’s disease (AD) have advanced our understanding the pathophysiology. In vivo studies retina using optical coherence tomography (OCT) complemented histological methods; however, lack standardisation in OCT methodologies for AD has led to significant variations results different studies. A literature search PubMed and Scopus been performed review methods used these analyse methodological characteristics each study. addition, some recommendations are offered...
Purpose.: To analyze the effects of different levels intraocular pressure (IOP) in macroglia ocular hypertension (OHT) and contralateral eyes at 3 weeks after laser photocoagulation compare these with age-matched control rats. Methods.: Adult Sprague-Dawley rats were divided into an (naive) group OHT group. Retinas processed as whole mounts immunostained GFAP for analysis retinal macroglia. Results.: The area retina occupied by astrocytes (AROA) was quantified. immunostaining showed common...
In Alzheimer’s disease (AD), vascular changes could be caused by amyloid beta (Aβ) aggregates replacing the contractile smooth musculature of arteriole walls. These happen in brain network, but also eye, and are related to decreased density low blood flow. patients with disease, thinning choroid retina has been shown. The aim this prospective study was assess retinal choroidal systems, analyzing thickness optical coherence tomography (OCT), foveal avascular zone (FAZ) OCT-angiography (OCTA),...
Signaling mediated by cytokines and chemokines is involved in glaucoma-associated neuroinflammation the damage of retinal ganglion cells (RGCs). Using multiplexed immunoassay immunohistochemical techniques a glaucoma mouse model at different time points after ocular hypertension (OHT), we analyzed (i) expression pro-inflammatory cytokines, anti-inflammatory BDNF, VEGF, fractalkine; (ii) number Brn3a+ RGCs. In OHT eyes, there was an upregulation IFN-γ days 3, 5, 15; IL-4 1, 7 IL-10 3 5...
Aims/Purpose: Transgenic mouse models of Alzheimer's disease (AD) have shown a correlation between retinal abnormalities and brain pathology. The APP NL‐F/NL‐F murine humanized AD model accurately replicates many pathological features seen in patients. This study aims to analyze, using manual segmentation by optical coherence tomography (OCT), the changes occurring several layers at 6, 9, 12, 15, 17, 20 months age, comparison C57BL/6J mice (WT). Methods: A diachronic was conducted OCT for...
Aims/Purpose: Transgenic mouse models of Alzheimer's disease (AD) have shown a correlation between retinal abnormalities and brain pathology. The APP NL‐F/NL‐F murine humanized AD model accurately replicates many pathological features seen in patients. This study aims to analyze, using manual segmentation by optical coherence tomography (OCT), the changes occurring several layers at 6, 9, 12, 15, 17, 20 months age, comparison C57BL/6J mice (WT). Methods: A diachronic was conducted OCT for...
Aims/Purpose: Treatments for glaucoma damage have focused on lowering intraocular pressure (IOP). However, despite controlled IOP, the neurodegenerative process continues. Therefore, it is necessary to study new molecules with neuroprotective properties treatment. The aim of this was investigate survival retinal ganglion cells (RGCs) in an experimental model ocular hypertension (OHT) following administration a combination citicoline and coenzyme Q10 (COQUN Combo). Methods: Four groups Swiss...
Aims/Purpose: In Alzheimer's disease (AD), there are histopathological aspects that cannot be studied in patients, demanding the use of murine models disease. The APP NL‐F/NL‐F model reproduces pathophysiology quite faithfully. aim present study was to analyze retinal vascular structures and thickness an AD at different ages compares these findings age‐matched wild type (WT) controls. Methods: A diachronic conducted using spectral‐domain OCT angiography (SD‐OCTA) AngioTool software assess...
Aims/Purpose: To investigate the anti‐inflammatory effects of citicoline and coenzyme Q10 (COQUN Combo) in an experimental model ocular hypertension (OHT). Methods: Swiss albino mice were used: vehicle control (Vehicle n = 6), COQUN Combo 5), laser (LG+Veh, (LG+COQUN Combo, 6). was administered daily with gelatin 15 days prior to induction 3 post‐induction LG+COQUN groups. The Vehicle LG+Veh groups received neutral gelatin. OHT induced left eye by photocoagulation limbal episcleral veins,...