A5042460237- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Telomeres, Telomerase, and Senescence
- Prenatal Screening and Diagnostics
- Advanced biosensing and bioanalysis techniques
- Microtubule and mitosis dynamics
- Redox biology and oxidative stress
- Cancer-related gene regulation
- Cancer Research and Treatments
Friedrich Miescher Institute
2019-2025
University of Basel
2024
Kurchatov Institute
2018-2023
Institute of Molecular Genetics
2017-2020
Institute of Bioorganic Chemistry
2018
Russian Academy of Sciences
2018
Lomonosov Moscow State University
2017-2018
Abstract In the germ line and during early embryogenesis, DNA methylation (DNAme) undergoes global erasure re-establishment to support cell embryonic development. While DNAme acquisition male development is essential for setting genomic imprints, other intergenerational roles paternal in defining chromatin are unknown. Through conditional gene deletion of de novo methyltransferases Dnmt3a and/or Dnmt3b , we observe that DNMT3A primarily safeguards against hypomethylation undifferentiated...
Telomeric small RNAs related to PIWI-interacting (piRNAs) have been described in various eukaryotes; however, their role germline-specific telomere function remains poorly understood. Using a Drosophila model, we performed an in-depth study of the biogenesis telomeric piRNAs and homeostasis germline. To fully characterize piRNA clusters, integrated data obtained from analysis endogenous repeats, as well transgenes inserted into different subtelomeric regions. The RNA-seq strains carrying...
ABSTRACT DNA methylation (DNAme) serves a stable gene regulatory function in somatic cells (1). In the germ line and during early embryogenesis, however, DNAme undergoes global erasure re-establishment to support cell embryonic development (2). While de novo acquisition male is essential for setting genomic imprints, other intergenerational roles paternal defining chromatin after fertilization are unknown. To approach this question, we reduced levels of developing through conditional...
Expression of transposable elements in the germline is controlled by Piwi-interacting (pi) RNAs produced genomic loci termed piRNA clusters and associated with Rhino, a heterochromatin protein 1 (HP1) homolog. Previously, we have shown that transgenes containing fragment I retrotransposon form de novo Drosophila providing suppression I-element activity. We noted identical located different sites vary considerably production classified them as "strong" "weak" clusters. Here, investigated what...
Insulators are architectural elements implicated in the organization of higher-order chromatin structures and transcriptional regulation. However, it is still unknown how insulators contribute to Drosophila telomere maintenance. Although telomeric retrotransposons HeT-A TART occupy a common genomic niche, they regulated independently. believed provide reverse transcriptase activity, whereas transcripts serve as template for elongation. Here, we report that insulator complexes associate with...
The study of the telomeric complex in oogenesis and early development is important for understanding mechanisms which maintain genome integrity. Telomeric transcripts are key components essential regulation telomere function. We biogenesis generated by major Drosophila repeat HeT-A with disrupted silencing. In wild type ovaries, expression downregulated Piwi-interacting RNAs (piRNAs). By repressing piRNA pathway, we show that overexpressed interact their product, RNA-binding protein...
Transgenes containing a fragment of the I retrotransposon represent powerful model piRNA cluster de novo formation in Drosophila germline. We revealed that same transgenes located at different genomic loci form clusters with various capacity small RNA production. Transgenic are not established pathway mutants. However, wild-type context, endogenous ancestral I-related piRNAs heterochromatinize and convert I-containing into piRNA-producing loci. Here, we address how quantitative level...
Piwi-interacting RNAs (piRNAs) control transposable element (TE) activity in the germline. piRNAs are produced from single-stranded precursors transcribed distinct genomic loci, enriched by TE fragments and termed piRNA clusters. The specific chromatin organization transcriptional regulation of Drosophila germline-specific clusters ensure transcription processing precursors. TEs harbour various regulatory elements that could affect cluster integrity. One such is suppressor-of-hairy-wing...
Abstract Telomeric small RNAs related to PIWI-interacting (piRNAs) were discovered in different species, however, their role germline-specific telomere function remains poorly understood. Using a Drosophila model, we show that the piRNA pathway provides strong mechanism of homeostasis. We telomeric retrotransposon arrays belong unique class dual-strand clusters whose transcripts, required for elongation, serve simultaneously as precursors and only targets. However, ability produce piRNAs...