A5020453750- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Nuclear Structure and Function
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- Glycosylation and Glycoproteins Research
- Invertebrate Immune Response Mechanisms
- Plant Virus Research Studies
- Genetics, Aging, and Longevity in Model Organisms
- Enzyme Production and Characterization
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- Genomics and Phylogenetic Studies
- Economic and Technological Developments in Russia
- Silk-based biomaterials and applications
- Microtubule and mitosis dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant Molecular Biology Research
- Transgenic Plants and Applications
- DNA and Nucleic Acid Chemistry
- Economic and Business Development Strategies
Kurchatov Institute
1996-2024
Institute of Molecular Genetics
2014-2023
Czech Academy of Sciences, Institute of Molecular Genetics
2022
Russian Academy of Sciences
1996-2021
Recent advances enabled by the Hi-C technique have unraveled many principles of chromosomal folding that were subsequently linked to disease and gene regulation. In particular, revealed chromosomes animals are organized into topologically associating domains (TADs), evolutionary conserved compact chromatin influence expression. Mechanisms underlie partitioning genome TADs remain poorly understood. To explore TAD in Drosophila melanogaster , we performed poly(A) + RNA-seq four cell lines...
Abstract How the nuclear lamina (NL) impacts on global chromatin architecture is poorly understood. Here, we show that NL disruption in Drosophila S2 cells leads to compaction and repositioning from envelope. This increases density a fraction of topologically-associating domains (TADs) enriched active enhances interactions between inactive chromatin. Importantly, upon NL-associated TADs become more acetylated at histone H3 less compact, while background transcription derepressed. Two-colour...
Piwi-interacting RNAs (piRNAs) and Piwi proteins have the evolutionarily conserved function of silencing repetitive genetic elements in germ lines. The founder subfamily, Drosophila nuclear protein, was also shown to be required for maintenance germ-line stem cells (GSCs). Hence, null mutant piwi females exhibit two types abnormalities, overexpression transposons severely underdeveloped ovaries. It remained unknown whether failure GSC is related transposon derepression or if self-renewal...
Abstract Mammalian and Drosophila genomes are partitioned into topologically associating domains (TADs). Although this partitioning has been reported to be functionally relevant, it is unclear whether TADs represent true physical units located at the same genomic positions in each cell nucleus or emerge as an average of numerous alternative chromatin folding patterns a population. Here, we use single-nucleus Hi-C technique construct high-resolution maps individual genomes. These demonstrate...
The ubiquitin ligase receptor Dora, the Drosophila homolog of ZSWIM8, is a key component target-directed microRNA degradation (TDMD) pathway. Previous studies have implicated TDMD - and, consequently, ZSWIM8/Dora in various developmental processes. Here, we investigate role Dora oogenesis, focusing on its function ovarian somatic cells. We generated fly strain with an endogenously tagged protein and observed presence both germline follicular cells ovaries. Somatic knockdown dora revealed...
Piwi in a complex with Piwi-interacting RNAs (piRNAs) triggers transcriptional silencing of transposable elements (TEs) Drosophila ovaries, thus ensuring genome stability. To do this, must scan the nascent transcripts genes and TEs for complementarity to piRNAs. The mechanism this scanning is currently unknown. Here we report DamID-seq mapping multiple chromosomal domains somatic cells ovaries. These significantly overlap genomic regions tethered Nuclear Pore Complexes (NPCs). Accordingly,...
Theoretical models suggest that gene silencing at the nuclear periphery may involve "closing" of chromatin by transcriptional repressors, such as histone deacetylases (HDACs). Here we provide experimental evidence confirming these predictions. Histone acetylation, compactness, and repression in lamina-interacting multigenic domains were analyzed Drosophila S2 cells which B-type lamin, diverse HDACs, lamina-associated proteins downregulated dsRNA. Lamin depletion resulted decreased...
In most mammalian cell lines, chromatin located at the nuclear periphery is represented by condensed heterochromatin, as evidenced microscopy observations and DamID mapping of lamina-associated domains (LADs) enriched in dimethylated Lys9 histone H3 (H3K9me2). However, Kc167 culture, only Drosophilla type where LADs have previously been mapped, they are neither H3K9me2-enriched nor overlapped with heterochromatin protein 1a (HP1a). Here, using type-specific we mapped genome-wide LADs, HP1a...
Insulators are architectural elements implicated in the organization of higher-order chromatin structures and transcriptional regulation. However, it is still unknown how insulators contribute to Drosophila telomere maintenance. Although telomeric retrotransposons HeT-A TART occupy a common genomic niche, they regulated independently. believed provide reverse transcriptase activity, whereas transcripts serve as template for elongation. Here, we report that insulator complexes associate with...
A secreted glycoprotein (GP) with apparent molecular mass of 90 kDa produced by cultured embryonic cells Drosophila melanogaster was isolated and partially characterized. GP is enriched Ser + Thr Pro residues that constitute up to 30% the total number amino acids. An abundant carbohydrate moiety (40% mass) mainly represented vertebrate mucin‐type O‐linked disaccharide units Gal(β1–3)‐GalNAc, occupying about a half rendering molecule high resistance protease action. few N ‐glycans are also...
Eukaryotic genomes harbor hundreds of rRNA genes, many which are transcriptionally silent. However, little is known about selective regulation individual rDNA units. In Drosophila melanogaster, some repeats contain insertions the R2 retrotransposon, capable to be transcribed only as part pre-rRNA molecules. units with usually inactivated, although expression may beneficial in cells decreased copy number. Here we found that R2-inserted enriched HP1a and H3K9me3 repressive mark, whereas...
In Drosophila ovarian somatic cells, PIWI-interacting small RNAs (piRNAs) against transposable elements are mainly produced from the ∼180-kb flamenco ( flam) locus. flam transcripts gathered into foci, located close to nuclear envelope, and processed piRNAs in cytoplasmic Yb bodies. The mechanism of body formation remains unknown. Using RNA fluorescence situ hybridization, we found that follicle cells ovaries 5′-ends usually proximity envelope outside bodies, whereas their extended...
In the Drosophila ovary, somatic escort cells (ECs) form a niche that promotes differentiation of germline stem cell (GSC) progeny. The piRNA (Piwi-interacting RNA) pathway, which represses transposable elements (TEs), is required in ECs to prevent accumulation undifferentiated germ (germline tumor phenotype). soma-specific cluster flamenco (flam) produces substantial part piRNAs. Here, we characterized biological effects TE activation on flam mutants. We revealed choice between normal and...
Telomeres are nucleoprotein complexes that protect the ends of eukaryotic linear chromosomes from degradation and fusions. Telomere dysfunction leads to cell growth arrest, oncogenesis, premature aging. Telomeric RNAs have been found in all studied species; however, their functions biogenesis not clearly understood. We mechanisms development disorders observed upon overexpression telomeric repeats Drosophila . In somatic cells, retrotransposon HeT-A is cytotoxic accumulation Gag near...
Abstract The nascent polypeptide-associated complex (NAC) consisting of α- and β-subunits is an essential ribosome-associated protein conserved in eukaryotes. NAC a ubiquitously expressed co-translational regulator folding sorting providing for homeostasis cellular proteins. Here we report on discovering the germline-specific NACαβ paralogs (gNACs), whose β-subunits, non-distinguishable by ordinary immunodetection, are encoded five highly homologous gene copies, while α-subunit single αNAC...
Abstract An RNA interference-based method was proposed to achieve an inducible knockdown of genes essential for cell viability. In the method, a genetic cassette in which copper ion-dependent metallothionein promoter controls expression siRNA precursor is inserted into genomic pre-integrated transgene by CRIPSR/Cas9 technology. The endogenous source allows gene cultures that are refractory conventional transfection with exogenous siRNA. efficiency demonstrated Drosophila ovarian somatic...
An RNA interference-based method was proposed to achieve an inducible knockdown of genes essential for cell viability. In the method, a genetic cassette in which copper ion-dependent metallothionein promoter controls expression siRNA precursor is inserted into genomic pre-integrated transgene by CRIPSR/Cas9 technology. The endogenous source allows gene cultures that are refractory conventional transfection with exogenous siRNA. efficiency demonstrated Drosophila ovarian somatic culture (OSC)...