A5056802383- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Nuclear Structure and Function
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Chromosomal and Genetic Variations
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Protein Hydrolysis and Bioactive Peptides
- Peptidase Inhibition and Analysis
- Neuropeptides and Animal Physiology
- Bacteriophages and microbial interactions
- Genomics and Rare Diseases
Siberian Branch of the Russian Academy of Sciences
2011-2021
Institute of Molecular and Cell Biology
2019
Russian Academy of Sciences
2009-2018
Institute of Chemical Biology and Fundamental Medicine
2011
Piwi in a complex with Piwi-interacting RNAs (piRNAs) triggers transcriptional silencing of transposable elements (TEs) Drosophila ovaries, thus ensuring genome stability. To do this, must scan the nascent transcripts genes and TEs for complementarity to piRNAs. The mechanism this scanning is currently unknown. Here we report DamID-seq mapping multiple chromosomal domains somatic cells ovaries. These significantly overlap genomic regions tethered Nuclear Pore Complexes (NPCs). Accordingly,...
Massively parallel reporter assays (MPRAs) enable high-throughput functional evaluation of various DNA regulatory elements and their mutant variants. The are based on construction highly diverse plasmid libraries containing two variable fragments, a region interest (a sequence under study; ROI) barcode (BC) used to uniquely tag each ROI, which separated by constant spacer sequence. sequences BC–ROI combinations present in the may be either known priori or not. In latter case, it is necessary...
In most mammalian cell lines, chromatin located at the nuclear periphery is represented by condensed heterochromatin, as evidenced microscopy observations and DamID mapping of lamina-associated domains (LADs) enriched in dimethylated Lys9 histone H3 (H3K9me2). However, Kc167 culture, only Drosophilla type where LADs have previously been mapped, they are neither H3K9me2-enriched nor overlapped with heterochromatin protein 1a (HP1a). Here, using type-specific we mapped genome-wide LADs, HP1a...
Expression of the CNDP2 gene is frequently up- or down-regulated in different types human cancers. However, how product this involved cell growth and proliferation poorly understood. Moreover, our knowledge functions orthologs well-established model organisms scarce. In particular, function D. melanogaster ortholog CNDP2, encoded by CG17337 (hereafter referred to as dCNDP2), still unknown. This study was aimed at developing a set genetic molecular tools roles dCNDP2. We generated dCNDP2 null...
The discovery of the position effect variegation phenomenon and subsequent comprehensive analysis its molecular mechanisms led to understanding that local chromatin composition has a dramatic on gene activity. To study this in high-throughput mode at genome-wide level, Thousands Reporters Integrated Parallel (TRIP) approach based usage barcoded reporter constructs was recently developed. Here we describe construction quality checks high-diversity plasmid libraries supposed be used for...
Massively parallel reporter assays (MPRAs) enable high-throughput functional evaluation of numerous DNA regulatory elements and/or their mutant variants. The are based on the construction plasmid libraries containing two variable parts, a region interest (ROI) and barcode (BC), located outside within transcription unit, respectively. Importantly, each molecule in such highly diverse library is characterized by unique BC–ROI association. constructs delivered to target cells expression BCs at...
The nucleolus is a dynamic non-membrane-bound nuclear organelle, which plays key roles not only in ribosome biogenesis but also many other cellular processes. Consistent with its multiple functions, the has been implicated human diseases, including cancer and degenerative pathologies of nervous system heart. Here, we report characterization Drosophila Non3 (Novel nucleolar protein 3) gene, encodes homologous to Brix domain-containing Rpf2 that shown control ribosomal RNA (rRNA) processing....