A5004268894- Neuroendocrine regulation and behavior
- Gut microbiota and health
- RNA Research and Splicing
- Olfactory and Sensory Function Studies
- Helicobacter pylori-related gastroenterology studies
- Human Health and Disease
- Neurobiology and Insect Physiology Research
- CRISPR and Genetic Engineering
- Digestive system and related health
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Probiotics and Fermented Foods
- Animal Genetics and Reproduction
- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Tryptophan and brain disorders
- Adipose Tissue and Metabolism
- Lipid Membrane Structure and Behavior
- Reproductive System and Pregnancy
- Food Industry and Aquatic Biology
- DNA Repair Mechanisms
- Pluripotent Stem Cells Research
- Nutrition and Health Studies
Novosibirsk State Agricultural University
2021-2025
Erasmus MC
2009-2025
Russian Academy of Sciences
2008-2025
Institute of Molecular and Cell Biology
2014-2024
Siberian Branch of the Russian Academy of Sciences
2014-2024
Scientific Research Institute of Biochemistry
2024
Siberian Research Institute of Agriculture and Peat
2019-2021
Institute of Physiology and Basic Medicine
2019-2020
Institute of Cytology and Genetics
2014-2019
Erasmus University Rotterdam
2012-2014
The disruption of the protective intestinal barrier-the 'leaky gut'-is a common complication inflammatory bowel disease. There is limited data on mechanisms barrier upon low-grade inflammation characteristic patients with disease in clinical remission. Thus, animal models that recapitulate complexity chronic vivo are particular interest. In this study, we used Mucin-2 (Muc2) knockout mice predisposed to colitis study inflammation. We 4-kDa FITC-Dextran assay and transmission electron...
Drosophila GMP synthetase binds ubiquitin-specific protease 7 (USP7) and is required for its ability to deubiquitylate histone H2B. Previously, we showed that the GMPS/USP7 complex cooperates with Polycomb silencing system through removal of active ubiquitin mark from H2B (H2Bub). Here, explored interplay between GMPS USP7 further assessed their role in hormone-regulated gene expression. Genetic analysis established a strong cooperation USP7, which counteracted by ligase BRE1. Loss either or...
Pathogenic variants in the ubiquitin-specific protease 7 ( USP7 ) gene cause a neurodevelopmental disorder called Hao-Fountain syndrome. However, it remains unclear which of USP7's pleiotropic functions are relevant for neurodevelopment. Here, we present combination quantitative proteomics, transcriptomics, and epigenomics to define regulatory circuitry during neuronal differentiation. activity is required transcriptional programs that direct both differentiation embryonic stem cells into...
Mouse genome modification requires costly equipment and highly skilled personnel to manipulate zygotes. A number of zygote electroporation techniques were reported be efficient in gene delivery. One these methods called i-GONAD (improved Genome-editing via Oviductal Nucleic Acids Delivery) describes electroporation-based transfer zygotes utero. Here we adopted this technology develop an easy-to-use cost-effective pipeline enabling mouse genome-editing from scratch with minimal requirements...
Dam identification (DamID) is a powerful technique to generate genome-wide maps of chromatin protein binding. Due its high sensitivity, it particularly suited study the genome interactions proteins in small tissue samples model organisms such as Drosophila. Here, we report an intein-based approach tune expression level and Dam-fusion Drosophila by addition ligand fly food. This helps suppress possible toxic effects Dam. In addition, describe strategy for genetically controlled specific cell...
Growing evidence suggests that intestinal mucosa homeostasis impacts immunity, metabolism, the Central Nervous System (CNS), and behavior. Here, we investigated effect of monosaccharide fucose on inflammation, microbiota, social behavior in Dextran Sulfate Sodium (DSS)-induced chronic colitis mouse model. Our data show is accompanied by decrease serum tryptophan level depletion specifically tryptophan-producing E. coli Bifidobacterium. These changes are associated with defects male such as a...
Inflammatory bowel diseases (IBD) are chronic and relapsing inflammatory disorders of the gastrointestinal tract with complex etiology no strategies for complete cure. IBD often complicated by mental like anxiety depression, indicating substantial shifts in microbiota gut-brain axis. However, mechanisms connecting to still under debate. Here we use Muc2 knockout mouse model colitis uncouple effects intestinal on host behavior from inflammation gut. male mice exhibit high exploratory...
ATP-dependent chromatin-remodeling complexes (remodelers) are essential regulators of chromatin structure and gene transcription. How remodelers can act in a gene-selective manner has remained enigmatic. A yeast two-hybrid screen for proteins binding the Drosophila transcription factor Tramtrack69 (TTK69) identified MEP1. Proteomic characterization revealed that MEP1 is tightly associated subunit NuRD remodeler, harboring Mi2 enzymatic core ATPase. In addition, we fly homolog human Deleted...
Proper control of DNA replication is essential to ensure faithful transmission genetic material and prevent chromosomal aberrations that can drive cancer progression developmental disorders. regulated primarily at the level initiation under strict cell-cycle regulation. Importantly, highly influenced by cues. In Drosophila, specific regions genome are repressed for during differentiation SNF2 domain-containing protein SUUR through an unknown mechanism. We demonstrate recruited active forks...
Dietary composition substantially determines human health and affects complex diseases, including obesity, inflammation cancer. Thus, food supplements have been widely used to accommodate dietary the needs of individuals. Among promising are phospholipids (PLs) that commonly found as natural ingredients emulsifier additives. The aim present study was evaluate effect major PLs on morphology intestinal epithelial cells upon short-term long-term high-dose feeding in mice. In report, high PL...
In vitro models are of great importance in advancing our understanding human diseases, especially complex disorders with unknown etiologies like inflammatory bowel diseases (IBD). One the key IBD features is increased intestinal permeability. The disruption barrier can occur due to a destructive response involving cell death. Alternatively, proteins that form tight junctions (TJ) fail function complexes and promote epithelial disruption. mechanisms behind this process not fully understood....
SUUR (Suppressor of Under-Replication) protein is responsible for late replication and, as a consequence, DNA underreplication intercalary and pericentric heterochromatin in Drosophila melanogaster polytene chromosomes. However, the mechanism by which slows down process not clear. To identify possible partners we performed yeast two-hybrid screen using full-length bait. This identified HP1, well-studied protein, strong interactor. Furthermore, have determined that central region necessary...
Intestinal mucus protects epithelial and immune cells from the gut resident microorganisms, provides growth-promoting factors as mucus-derived O-glycans for beneficial bacteria. A lack of intestinal protective results in changes commensal microflora composition, mucosal system reprogramming, inflammation. Previous work has shown that fucose, terminal glycan chain component glycoprotein Mucin2, fucoidan polysaccharides have an anti-inflammatory effect some mouse models colitis. This study...
Abstract Understanding the complex dynamics of social communication behaviors, such as exploration, communication, courtship, mating, and aggression in animal models, is crucial to reveal key neural hormonal mechanisms underlying these behaviors. The two‐intruders test designed investigate residents' behavior toward both male female intruders within home cage male. During this imitating natural conditions, several aspects interaction were investigated: Exploration, aggressive behavior. As...
Expression of the CNDP2 gene is frequently up- or down-regulated in different types human cancers. However, how product this involved cell growth and proliferation poorly understood. Moreover, our knowledge functions orthologs well-established model organisms scarce. In particular, function D. melanogaster ortholog CNDP2, encoded by CG17337 (hereafter referred to as dCNDP2), still unknown. This study was aimed at developing a set genetic molecular tools roles dCNDP2. We generated dCNDP2 null...
Infections with Helicobacter spp. are known to have serious effects on rodent health, especially in immunocompromised animals. In this study three approaches were used eradicate infection mice a deficiency intestinal proteoglycan (mucin2), namely triple oral antibiotic therapy (amoxicillin, clarithromycin and metronidazole), cross-fostering of neonatal pups by surrogate mothers negative for spp., vitro fertilization (IVF) embryo transfer into Helicobacter-free mothers. However, mucin2 was...
The mucus layer in the intestine plays a critical role regulation of host-microbe interactions and maintaining homeostasis. Disruptions due to genetic, environmental, or immune factors may lead inflammatory bowel diseases (IBD). IBD frequently are accompanied with infections, therefore treated antibiotics. Hence, it is important evaluate risks antibiotic treatment individuals vulnerable gut barrier chronic inflammation. Mice knockout Muc2 gene, encoding main glycoprotein component mucus,...