A5052387712- Nuclear Structure and Function
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Microtubule and mitosis dynamics
- Olfactory and Sensory Function Studies
- RNA Interference and Gene Delivery
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Genetic Neurodegenerative Diseases
- Diet and metabolism studies
- MicroRNA in disease regulation
- Mitochondrial Function and Pathology
- Extracellular vesicles in disease
- Advanced Drug Delivery Systems
- Helicobacter pylori-related gastroenterology studies
- Advanced Chemical Sensor Technologies
- Advanced Electron Microscopy Techniques and Applications
- Genetics, Aging, and Longevity in Model Organisms
- Reproductive Biology and Fertility
- Endoplasmic Reticulum Stress and Disease
- Tissue Engineering and Regenerative Medicine
- Molecular Communication and Nanonetworks
- Blood disorders and treatments
- Inhalation and Respiratory Drug Delivery
- Barrier Structure and Function Studies
Institute of Cytology and Genetics
2007-2025
Siberian Branch of the Russian Academy of Sciences
2018-2025
Russian Academy of Sciences
2007-2025
Novosibirsk State University
2017-2024
Bioinformatics Institute
2021
The disruption of the protective intestinal barrier-the 'leaky gut'-is a common complication inflammatory bowel disease. There is limited data on mechanisms barrier upon low-grade inflammation characteristic patients with disease in clinical remission. Thus, animal models that recapitulate complexity chronic vivo are particular interest. In this study, we used Mucin-2 (Muc2) knockout mice predisposed to colitis study inflammation. We 4-kDa FITC-Dextran assay and transmission electron...
Reticulon 4a (Rtn4a) is a membrane protein that shapes tubules of the endoplasmic reticulum (ER). The ER attached to nuclear envelope (NE) during interphase and has role in post mitotic/meiotic NE reassembly. We speculated Rtn4a dynamics. Using immuno-electron microscopy we found located at junctions between membranes cytoplasm, cytoplasmic outer growing Xenopus oocyte nuclei. assembly egg extracts, localises edges are flattening onto chromatin. These results demonstrate locates regions high...
Extracellular vesicles provide cell-to-cell communication and have great potential for use as therapeutic carriers. This study was aimed at the development of an extracellular vesicle-based system nucleic acid delivery. Three types nanovesicles were assayed oligonucleotide carriers: mesenchymal stem cell-derived mimics prepared either by cell treatment with cytochalasin B or vesicle generation from plasma membrane. Nanovesicles loaded a DNA freezing/thawing, sonication, permeabilization...
Dietary composition substantially determines human health and affects complex diseases, including obesity, inflammation cancer. Thus, food supplements have been widely used to accommodate dietary the needs of individuals. Among promising are phospholipids (PLs) that commonly found as natural ingredients emulsifier additives. The aim present study was evaluate effect major PLs on morphology intestinal epithelial cells upon short-term long-term high-dose feeding in mice. In report, high PL...
Huntington’s disease (HD) is a severe neurodegenerative disorder caused by CAG triplet expansion in the first exon of HTT gene. Here we report introduction an HD mutation into genome healthy human embryonic fibroblasts through CRISPR/Cas9-mediated homologous recombination. We verified specificity created HTT-editing system and confirmed absence undesirable genomic modifications at off-target sites. showed that both mutant control isogenic induced pluripotent stem cells (iPSCs) derived...
Modeling of neurodegenerative diseases in vitro holds great promise for biomedical research. Human cell lines harboring a mutations disease-causing genes are thought to recapitulate early stages the development an inherited disease. Modern genome-editing tools allow researchers create isogenic clones with identical genetic background providing adequate "healthy" control and pharmacological experiments. Here, we generated mutant 150 CAG repeats first exon huntingtin (HTT) gene using...
The main advantage of extracellular vesicles (EVs) as a drug carrier system is their low immunogenicity and internalization by mammalian cells. EVs are often considered cell-specific delivery system, but the production preparative amounts for therapeutic applications challenging due to laborious isolation purification procedures. Alternatively, mimetic prepared from cellular plasma membrane can be used in same way natural EVs. For example, cytoskeleton-destabilizing agent, such cytochalasin...
Exposure to nanomaterials is considered as one of the risk factors for neurodegenerative pathology. In vitro inorganic nanoparticles (NPs) absorb intrinsically disordered proteins, many which are constituents stress-granules (SGs). SGs normally form in response cellular stress and, here, we addressed whether selected NPs could trigger formation cells. To this end, have tested a series their ability induce human glioblastoma and fibroblast cell lines. Among NPs, only Mn3O4 triggered...
Cohen syndrome is an autosomal recessive disorder caused by VPS13B (COH1) gene mutations. This significantly underdiagnosed and characterized intellectual disability, microcephaly, autistic symptoms, hypotension, myopia, retinal dystrophy, neutropenia, obesity. regulates intracellular membrane transport supports the Golgi apparatus structure, which critical for neuron formation. We generated induced pluripotent stem cells from two patients with pronounced manifestations of differentiated...
There is no doubt that various nanoparticles (NPs) can enter the brain from nasal cavity. It assumed NPs penetrate blood into central nervous system (CNS) only by breaking blood–brain barrier (BBB). The accumulation of in CNS provoke many neurological diseases; therefore, understanding its mechanisms both academic and practical interest. Although hitting surface lungs bloodstream, accumulate mucous membranes, including mucosa. Thus, we cannot rule out ability to be transported bloodstream...
Protein deficiency in the diet during pregnancy and lactation has a serious impact on offspring by programming predisposition to such diseases as hypertension type 2 diabetes mellitus. In our study, we examined liver ultrastructure of rat pups at ages 2, 21, 40 days with maternal protein deficiency. Body weight progressively lagged behind control throughout experiment, timing eye opening indicated slowdown development. 2-day-old animals, proportion hematopoietic cells early stages...