The disruption of the protective intestinal barrier-the 'leaky gut'-is a common complication inflammatory bowel disease. There is limited data on mechanisms barrier upon low-grade inflammation characteristic patients with disease in clinical remission. Thus, animal models that recapitulate complexity chronic vivo are particular interest. In this study, we used Mucin-2 (Muc2) knockout mice predisposed to colitis study inflammation. We 4-kDa FITC-Dextran assay and transmission electron...

Growing evidence suggests that intestinal mucosa homeostasis impacts immunity, metabolism, the Central Nervous System (CNS), and behavior. Here, we investigated effect of monosaccharide fucose on inflammation, microbiota, social behavior in Dextran Sulfate Sodium (DSS)-induced chronic colitis mouse model. Our data show is accompanied by decrease serum tryptophan level depletion specifically tryptophan-producing E. coli Bifidobacterium. These changes are associated with defects male such as a...

Inflammatory bowel diseases (IBD) are chronic and relapsing inflammatory disorders of the gastrointestinal tract with complex etiology no strategies for complete cure. IBD often complicated by mental like anxiety depression, indicating substantial shifts in microbiota gut-brain axis. However, mechanisms connecting to still under debate. Here we use Muc2 knockout mouse model colitis uncouple effects intestinal on host behavior from inflammation gut. male mice exhibit high exploratory...

In vitro models are of great importance in advancing our understanding human diseases, especially complex disorders with unknown etiologies like inflammatory bowel diseases (IBD). One the key IBD features is increased intestinal permeability. The disruption barrier can occur due to a destructive response involving cell death. Alternatively, proteins that form tight junctions (TJ) fail function complexes and promote epithelial disruption. mechanisms behind this process not fully understood....

Intestinal mucus protects epithelial and immune cells from the gut resident microorganisms, provides growth-promoting factors as mucus-derived O-glycans for beneficial bacteria. A lack of intestinal protective results in changes commensal microflora composition, mucosal system reprogramming, inflammation. Previous work has shown that fucose, terminal glycan chain component glycoprotein Mucin2, fucoidan polysaccharides have an anti-inflammatory effect some mouse models colitis. This study...

Does the genotype of surrogate mother modulate body composition and immunity her offspring?C57BL/6J (B6) progenies carried by immunodeficient NOD SCID (NS) mothers had increased adaptive but decreased innate, immune responsiveness in comparison with same offspring immunocompetent mothers, B6 BALB/c (C); also showed higher fat than others.Differences major histocompatibility complex (MHC) genes between foetus is considered as an important factor prenatal embryo development, whereas impact...

Infections with Helicobacter spp. are known to have serious effects on rodent health, especially in immunocompromised animals. In this study three approaches were used eradicate infection mice a deficiency intestinal proteoglycan (mucin2), namely triple oral antibiotic therapy (amoxicillin, clarithromycin and metronidazole), cross-fostering of neonatal pups by surrogate mothers negative for spp., vitro fertilization (IVF) embryo transfer into Helicobacter-free mothers. However, mucin2 was...

The mucus layer in the intestine plays a critical role regulation of host-microbe interactions and maintaining homeostasis. Disruptions due to genetic, environmental, or immune factors may lead inflammatory bowel diseases (IBD). IBD frequently are accompanied with infections, therefore treated antibiotics. Hence, it is important evaluate risks antibiotic treatment individuals vulnerable gut barrier chronic inflammation. Mice knockout Muc2 gene, encoding main glycoprotein component mucus,...

The reproducibility of results obtained with rodent models depends on the genetic purity strain and stability environment. However, another potential factor is changes in gut microbiota due to transmission mother's bacteria during embryo transfer. In this study, we demonstrate immune cell blood phenotype offspring 2 strains, C57BL/6JNskrc BALB/cJNskrc, from surrogate dams different genotypes. Interstrain transfer resulted a change number

The number of people with inflammatory bowel disease (IBD) is constantly increasing worldwide. main factors that have effects on the etiology are genetic, environmental and immunological. However, mechanism development effective treatment IBD not yet been found. Animal models help address these problems. most popular model considered to be transgenic in which individual genes knocked out. One such for study mice a null mutation Muc2 gene encoding Mucin-2 protein, involved formation protective...

Gut is inhabited by numerous of bacteria interacting with the immune system host. Disturbance gut homeostasis leads to various pathological states, which are be treated changing microbiota. The study demonstrate effects microbiota alteration on viability mice deficient barrier function in (Muc2-/-). susceptibility Muc2-/- suggesting as an appropriate model for testing approaches alter

The immune processes associated with the formation of resistance to pathogens in intestine depend on microbiome. maintenance homeostasis is provided by regulatory T-cells. In inflammatory bowel disease (IBD), both a disturbance T-regulatory function and changes microflora are observed. Aggravation accompanied various infections. However, pathobionts such as Helicobacter spp., can affect One genetic models for studying IBD Muc2 knockout mice. these mice, humans IBD, intestinal epithelial...

Acute inflammatory begins to activate of M1-macrophage by classical pathway. In the chronic form disease, body triggers an adaptive alternative pathway M2-macrophages. For example, number intestinal M2-macrophages increases in infl ammatory bowel disease (IBD) a form. We have got model mice that do not mature lymphocytes and these animals development IBD due absence mucosal barrier. obtained good reveals role macrophages IBD, can also be used understand effect pharmacological drugs cause...

Scent signals play an important role in the life of rodents. The scent opposite sex can modulate immunity. In mice populations with natural specific pathogens, males, a female leads to redistribution leukocytes between lung and blood, resistance influenza virus, decrease antibody production, but not development inflammation induced by bacterial endotoxins. This study demonstrates effect soiled bedding pathogen-free (SPF) status on percentage different types expression Nos2, Arg1, Foxp3...