A5020874063- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- Hereditary Neurological Disorders
- DNA and Nucleic Acid Chemistry
- Genetics, Bioinformatics, and Biomedical Research
- Neurological diseases and metabolism
- Plant Molecular Biology Research
- Insect Resistance and Genetics
- Plant Reproductive Biology
- Plant Genetic and Mutation Studies
- Genetic factors in colorectal cancer
- Endoplasmic Reticulum Stress and Disease
- Viral Infectious Diseases and Gene Expression in Insects
- Nuclear Receptors and Signaling
- Genetic Associations and Epidemiology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Advanced Scientific Research Methods
- Surface Roughness and Optical Measurements
Institute of Molecular and Cell Biology
2018-2024
Russian Academy of Sciences
2006-2023
Siberian Branch of the Russian Academy of Sciences
2003-2022
Novosibirsk State University
2008-2022
Institute of Chemical Biology and Fundamental Medicine
2011
Institute of Cytology and Genetics
2001-2008
University Health Network
2003
In D. melanogaster polytene chromosomes, intercalary heterochromatin (IH) appears as large dense bands scattered in euchromatin and comprises clusters of repressed genes. IH displays distinctly low gene density, indicative their particular regulation. Genes embedded replicate late the S phase become underreplicated. We asked whether localization organization these late-replicating domains is conserved a distinct cell type. Using published comprehensive genome-wide chromatin annotation...
Eukaryotic DNA replicates asynchronously, with discrete genomic loci replicating during different stages of S phase.
The structural and functional analyses of heterochromatin are essential to understanding how heterochromatic genes regulated centromeric chromatin is formed. Because the repetitive nature hampers its genome analysis, new approaches need be developed. Here, we describe how, in double mutants for Su(var)3-9 SuUR encoding two proteins heterochromatin, banded segments appear all polytene chromosomes due strong suppression under-replication pericentric regions. FISH on salivary gland from these...
SUUR (Suppressor of Under-Replication) protein is responsible for late replication and, as a consequence, DNA underreplication intercalary and pericentric heterochromatin in Drosophila melanogaster polytene chromosomes. However, the mechanism by which slows down process not clear. To identify possible partners we performed yeast two-hybrid screen using full-length bait. This identified HP1, well-studied protein, strong interactor. Furthermore, have determined that central region necessary...
Morphologically, polytene chromosomes of Drosophila melanogaster consist compact "black" bands alternating with less "grey" and interbands. We developed a comprehensive approach that combines cytological mapping data FlyBase-annotated genes novel tools for predicting cytogenetic features on the basis their protein composition determined genomic coordinates all black chromosome 2R. By PCNA immunostaining assay, we obtained replication timetable mapped. The results allowed us to compare timing...
<i>PS1</i> mutations are associated with classic Alzheimer's disease (AD); however, some families develop AD and spastic paraplegia (SP) brain pathology characterized by Aβ cotton wool plaques. The authors report a variant family the E280Q mutation. fact that same mutation can be found in patients either or argues favor of presence genetic modifier. have excluded this modifier effect originates from coding sequence variations three SP genes second other genes.
SUMMARY Among flowering plants, genome size varies remarkably, by >2200‐fold, and this variation depends on the loss gain of noncoding DNA sequences that form distinct heterochromatin complexes during interphase. In plants with giant genomes, most chromatin remains condensed interphase, forming a dense network threads called interphase chromonemata. Using super‐resolution light electron microscopy, we studied ultrastructure chromonemata after replication in root meristem nuclei Nigella...
In polytene chromosomes of Drosophila melanogaster, regions pericentric heterochromatin coalesce to form a compact chromocenter and are highly underreplicated. Focusing on study X chromosome heterochromatin, we demonstrate that loss either SU(VAR)3-9 histone methyltransferase activity or HP1 protein differentially affects the compaction different regions. Using set inversions breaking in background Su(var)3-9 mutations, show distal (blocks h26-h29) is only one within big "puff"-like...
Many late replicating regions are underreplicated in polytene chromosomes of Drosophila melanogaster. These contain silenced chromatin and overlap long syntenic blocks conserved gene order drosophilids. In this report we show that D. melanogaster the enriched with fast-evolving genes lacking homologs distant species such as mosquito or human, indicating phylogenetic conservation correlates replication timing status. without human located have higher nonsynonymous substitution rate tend to...
Drosophila chromosomes are organized into distinct domains differing in their predominant chromatin composition, replication timing and evolutionary conservation. We show on a genome-wide level that genes whose order has remained unaltered across 9 species display late frequently map to the regions of repressive chromatin. This observation is consistent with existence extensive replicate extremely have conserved gene genome. suggest such correspond handful complete at very end S phase....
Overexpression of Suppressor Underreplication protein (SUUR) induces giant reversible swellings in intercalary and pericentric heterochromatin salivary gland polytene chromosomes. Here, we demonstrate that morphology extent are highly dependent on the fixation conditions used: upon glutaraldehyde fixation, observed moderate decondensation heterochromatic regions, which was significantly more pronounced acetic-acid fixation. Swellings formed a PARP-independent fashion. Together with data...
In Drosophila salivary gland polytene chromosomes, a substantial portion of heterochromatin is underreplicated. The combination mutations SuURES and Su(var)3-906 results in the polytenization fraction unique moderately repeated sequences but has almost no effect on satellite DNA replication. Rap1 interacting factor 1 (Rif) protein conserved regulator replication timing, Drosophila, it affects underreplication chromosomes. We compared morphology pericentromeric regions labeling patterns situ...
Abstract Different genomic regions replicate at a distinct time during S-phase. The SuUR mutation alters replication timing and the polytenization level of intercalary pericentric heterochromatin in Drosophila melanogaster salivary gland polytene chromosomes. We analyzed different insects, identified conserved protein, substituted amino acid residues, studied effects mutations on SUUR function. orthologs were all sequenced drosophilids, highly divergent ortholog was found mosquito genome....
Abstract Regulation of DNA replication and copy number is necessary to promote genome stability maintain cell tissue function. regulated temporally in a process known as timing (RT). Rap1-interacting factor 1 (Rif1) key regulator RT has critical function control polyploid cells. Previously, we demonstrated that Rif1 functions with SUUR inhibit fork progression underreplication (UR) specific genomic regions. How Rif1-dependent factors into its ability UR unknown. By applying computational...