A5046671884- Advanced Proteomics Techniques and Applications
- Peptidase Inhibition and Analysis
- Plant-Microbe Interactions and Immunity
- Machine Learning in Bioinformatics
- Metabolomics and Mass Spectrometry Studies
- Studies on Chitinases and Chitosanases
- Light effects on plants
- Clinical Reasoning and Diagnostic Skills
- Algal biology and biofuel production
- Marine and coastal plant biology
- Phonocardiography and Auscultation Techniques
- Histone Deacetylase Inhibitors Research
- Simulation-Based Education in Healthcare
- Advanced Biosensing Techniques and Applications
- Antimicrobial agents and applications
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Ferrocene Chemistry and Applications
- Urban Green Space and Health
- Click Chemistry and Applications
- Amphibian and Reptile Biology
- Air Quality and Health Impacts
- Cancer-related gene regulation
- Microbial Natural Products and Biosynthesis
- Microbial Metabolism and Applications
- Effects of Environmental Stressors on Livestock
University of North Carolina at Chapel Hill
2021-2025
AstraZeneca (United States)
2024
scPharmaceuticals (United States)
2024
University of Michigan–Ann Arbor
2020-2022
C. S. Mott Children's Hospital
2016-2022
Michigan Medicine
2016-2020
Michigan United
2020
Villanova University
2015-2017
John Wiley & Sons (United States)
2016
Ecological Society of America
2016
Abstract In photosynthetic organisms light acts as an environmental signal to control their development and physiology, energy source drive the conversion of CO 2 into carbohydrates used for growth or storage. The main storage carbohydrate in green algae is starch, which accumulates during day broken down at night meet cellular demands. signalling role quality regulation starch accumulation remains unexplored. Here, we identify PHOTOTROPIN-MEDIATED SIGNALLING KINASE 1 (PMSK1) a key regulator...
Abstract In photosynthetic organisms, light acts as an environmental signal to control their development and physiology, well energy source drive the conversion of CO 2 into carbohydrates used for growth or storage. The main storage carbohydrate in green algae is starch, which accumulates during day broken down at night meet cellular demands. signaling role quality regulation starch accumulation remains unexplored. Here, we identify PHOTOTROPIN-MEDIATED SIGNALING KINASE 1 (PMSK1) a key...
Quaternary ammonium compounds (QACs) are a vital class of antiseptics. Recent investigations into their construction uncovering novel and potent multicationic variants. Based on trisQAC precedent, we have implemented scaffold-hopping approach to develop alternative QAC architectures that display 1-3 long alkyl chains in specific projections from cyclic branched core structures bearing 3-4 nitrogen atoms. The preparation 30 allowed for correlation scaffold structure with antimicrobial...
Abstract Background: PRMT5 is an epigenetic enzyme that catalyzes symmetric di-methylation of arginine (SDMA) multiple substrates regulate biological processes including RNA splicing and cell cycle. The role in controlling chromatin accessibility has been investigated some cancer contexts, but its non-small lung (NSCLC) global regulation not known. AstraZeneca developed MTA-cooperative inhibitor (AZD3470) selectively inhibits MTAP-null tumors currently Phase I clinical trial (NCT06130553,...
Disruptions to the microbiome can impact host health as exposure environmental contaminants. However, few studies have addressed how contaminants microbiome. We explored this question for frogs that breed in wetlands contaminated with fly ash, a by-product of coal combustion is enriched trace elements. found differences bacterial communities among ash-contaminated site and several reference wetlands. then experimentally assessed impacts ash on skin adult spring peepers (Pseudacris crucifer)....
Enterococcus faecalis is a Gram-positive bacterium that major cause of hospital-acquired infections due, in part, to its intrinsic resistance cell wall-active antimicrobials. One critical determinant this the transmembrane kinase IreK, which belongs penicillin-binding protein and serine/threonine kinase-associated family bacterial signaling proteins involved with regulation wall homeostasis. The activity IreK enhanced response stress, but direct substrates phosphorylation, leading...
Abstract Protein phosphorylation, which is one of the most important post-translational modifications (PTMs), involved in regulating myriad cellular processes. Herein, we present a novel deep learning based approach for organism-specific protein phosphorylation site prediction Chlamydomonas reinhardtii , model algal phototroph. An ensemble combining convolutional neural networks and long short-term memory (LSTM) achieves best performance predicting sites C. reinhardtii. Deemed...
Upon pathogen infection, receptors in plants will activate a localized immune response, the effector-triggered immunity (ETI), and systemic acquired response (SAR). Infection also induces oscillations redox environment of plant cells, triggering mechanisms involving sensitive cysteine residues that subsequently alter protein function. Arabidopsis thaliana thimet oligopeptidases TOP1 TOP2 are required for defense against pathogens oxidative stress response. Herein, we evaluated biochemical...
Protein homeostasis (proteostasis) is crucial for proper cellular function, including the production of peptides with biological functions through controlled proteolysis. Proteostasis has roles in maintenance and plant interactions environment under physiological conditions. Plant stress continues to reduce agricultural yields causing substantial economic losses; thus, it critical understand how plants perceive signals elicit responses survival. As previously shown Arabidopsis thaliana,...
Abstract Background: Acute myeloid leukemia (AML) is a prevalent adult leukemia. In the US, around 20,380 individuals were diagnosed with AML in 2023. Despite standard therapies, relapse and resistance persist. Cyclin-dependent kinase 9 (CDK9) has been recognized an actionable therapeutic target AML. The potent inhibitor, AZD4573, induces durable apoptosis suppresses tumor growth. Our study reveals that combining AZD4573 standard-of-care (SoC) treatments deepens cell death responses multiple...
Abstract In photosynthetic organisms light acts as an environmental signal to control their development and physiology, energy source drive the conversion of CO2 into carbohydrates used for growth or storage. The main storage carbohydrate in green algae is starch, which accumulates during day broken down at night meet cellular demands. signalling role quality regulation starch accumulation remains unexplored. Here, we report that model alga Chlamydomonas reinhardtii blue perceived by...
Abstract Protein phosphorylation is one of the most important post-translational modifications (PTMs) and involved in myriad cellular processes. Although many non-organism-specific computational site prediction tools a few for organism-specific exist, none are currently available Chlamydomonas reinhardtii. Herein, we present novel deep learning (DL) based approach protein reinhardtii , model algal phototroph. Our called Chlamy-EnPhosSite (based on ensemble combining convolutional neural...
Interactomics is an emerging field that seeks to identify both transient and complex-bound protein interactions are essential for metabolic functions.
Abstract Objective As part of quality improvement effort to decrease antibiotic use for presumed pneumonia (PNA) in our neonatal intensive care unit, we introduced minibronchoalveolar lavage (mBAL) obtain culture specimens. We evaluated the effect tracheal sampling method and results on use. Methods performed a retrospective review samples obtained by aspirates (TAs) mBAL between January 2017 April 2018. Noting time cultures were obtained, reviewed records determine if patients met Center...
Abstract Targeted proteolysis activities activated during the plant immune response catalyze synthesis of stable endogenous peptides. Little is known about their biogenesis and biological roles. Herein, we characterize an Arabidopsis thaliana mutant top1top2 in which targeted immune-active peptides drastically impaired effector-triggered immunity (ETI). For effective ETI, redox-sensitive thimet oligopeptidases TOP1 TOP2 are required. Quantitative mass spectrometry-based peptidomics allowed...
Proteostasis is essential for proper cellular function, including the production of peptides with biological functions via breakdown proteins/peptides through controlled proteolysis. contributes to maintenance and plant-environment interactions under physiological conditions. Plant stress continues reduce agricultural yields resulting in significant economic losses; thus, it crucial understand how plants perceive signals elicit responses survival. As previously shown Arabidopsis thaliana,...