A5013639072- Polyamine Metabolism and Applications
- Amino Acid Enzymes and Metabolism
- Cell Adhesion Molecules Research
- Aldose Reductase and Taurine
- Biopolymer Synthesis and Applications
- Epigenetics and DNA Methylation
- Metabolism and Genetic Disorders
- Animal Genetics and Reproduction
- GABA and Rice Research
- Cannabis and Cannabinoid Research
- Virus-based gene therapy research
- Cell death mechanisms and regulation
- Cancer Research and Treatments
- Cellular Mechanics and Interactions
- Cancer, Hypoxia, and Metabolism
- Chronic Myeloid Leukemia Treatments
- Bone and Dental Protein Studies
- Cancer-related Molecular Pathways
- HER2/EGFR in Cancer Research
- Cancer-related gene regulation
- Cutaneous Melanoma Detection and Management
- Cancer Cells and Metastasis
- Molecular Biology Techniques and Applications
- Health, psychology, and well-being
- Endoplasmic Reticulum Stress and Disease
University of Helsinki
2009-2020
American Journal Experts (United States)
2018
Helsinki University Hospital
2004-2015
Aava Medical Centre
2012
National Cancer Institute
2006
Center for Cancer Research
2006
Population Council
1985
Rockefeller University
1985
University of Eastern Finland
1973
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOxidation of spermidine and spermine in rat liver: purification properties polyamine oxidaseErkki HolttaCite this: Biochemistry 1977, 16, 1, 91–100Publication Date (Print):January 11, 1977Publication History Published online1 May 2002Published inissue 11 January 1977https://doi.org/10.1021/bi00620a015RIGHTS & PERMISSIONSArticle Views365Altmetric-Citations249LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article...
We have recently isolated, without using any inhibitors, a mutant of Chinese hamster ovary cell line which greatly overproduces ornithine decarboxylase in serum-free culture.Addition polyamines (putrescine, spermidine, or spermine, 10 WM) (1 mM), the precursor polyamines, to culture medium these cells caused rapid and extensive decay activity.At same time activity S-adenosylmethionine showed less pronounced decrease, Notably, polyamine concentrations used were optimal for growth no...
NIH 3T3 cells transformed with the human c-Ha-rasVal-12 oncogene showed markedly enhanced activity of ornithine decarboxylase (ODC), key enzyme polyamine biosynthesis, as compared their nontransformed counterparts. While in normal and c-Ha-ras proto-oncogene-transfected stimulation serum caused a transient induction ODC, transfected mutant ODC persisted at high levels for greatly extended periods time. The amounts immunoreactive protein roughly paralleled changes activity. augmentation...
A main function attributed to the BCL2 protein is its ability confer resistance against apoptosis. In addition constitutively high expression of BCL2, caused by gene rearrangement in follicular lymphomas, elevated has been found differentiating hematopoietic, neural, and epithelial tissues. To address question whether a cause or consequence cell differentiation, we used human neural-crest-derived tumor line, Paju, that undergoes spontaneous neural differentiation vitro. The Paju line...
1. Injections of sublethal doses methylglyoxal bis(guanylhydrazone), a potent inhibitor putrescine-activated S-adenosylmethionine decarboxylase in vitro, resulted after few days an immense increase the activity normal and regenerating rat liver thymus. The was at least partly due to marked lengthening half-life enzyme. 2. In thymus there also moderate stimulation ornithine (EC 4.1.1.17) accumulation tissue putrescine. 3. Injection bis(guanylhydrazone) into markedly decreased diamine oxidase...
1. The activation of human peripheral blood lymphocytes by phytohaemagglutinin in vitro was accompanied striking increases the concentrations natural polyamines putrescine, spermidine and spermine. 2. enhanced accumulation could be almost totally abolished dl-alpha-difluoromethylornithine, a newly discovered irreversible inhibitor l-ornithine decarboxylase (EC 4.1.1.17), or methylglyoxal bis(guanylhydrazone) {1,1'-[(methylethanediylidene)dinitrilo]diguanidine}, an S-adenosyl-l-methionine...
We have studied the ability of neu tyrosine kinase to induce a signal for activation cell growth-regulated genes. Serum-starved NIH 3T3 cells expressing an epidermal growth factor receptor (EGF-R)/neu construct encoding hybrid protein were stimulated with EGF and stimulation inducible genes followed at mRNA, protein, activity levels, compared corresponding responses in proto-oncogene oncogene cells. Induction expression jun mRNAs was immediate early effect stimulation, by marked increase...
We have found that overexpression of human ornithine decarboxylase (ODC) induces cell transformation in NIH 3T3 and Rat-1 cells (M. Auvinen, A. Paasinen, L. C. Andersson, E. Hölttä, Nature (London) 360:355-358, 1992). The ODC-transformed display increased levels tyrosine phosphorylation, particular a cluster 130-kDa proteins. Here we show one the proteins with enhanced phosphorylation ODC-overexpressing is previously described p130 substrate pp60v-src, known to associate also v-Crk...
We previously described an arginase-deficient, polyamine-dependent Chinese hamster ovary cell line which grows in serum-free medium. From this strain we isolated a new mutant that has no detectable catalytic ornithine decarboxylase activity. The cells contain, however, immunoreactive decarboxylase-like protein roughly the same quantity as parent strain. and strains also contain similar amounts of decarboxylase-mRNA hybridizable to specific cDNA. If polyamines are omitted from medium,...
Abstract The activities of L‐ornithine decarboxylase (EC 4.1.1.17) and S‐adenosyl‐L‐methionine 4.1.1.50) exhibited marked fluctuations in the livers rats fed 4‐dimethylaminoazobenzene. After 1 month dye feeding activity ornithine rose to a level about six times higher than that controls adenosyl‐methionine was more twice initial control level. These elevations were transient, returned near normal values within next 2 months. Four months after commencement azo‐dye diet, adenosylmethionine...