A5031665908- Biochemical and Molecular Research
- Microbial Natural Products and Biosynthesis
- Carbon Nanotubes in Composites
- Antibiotic Resistance in Bacteria
- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Polymer crystallization and properties
- Carbohydrate Chemistry and Synthesis
- Epoxy Resin Curing Processes
- Biochemical and Structural Characterization
- Genomics and Phylogenetic Studies
- Mechanical Behavior of Composites
- Enzyme Structure and Function
- Cancer therapeutics and mechanisms
- Glycosylation and Glycoproteins Research
- Polymer Nanocomposites and Properties
- RNA and protein synthesis mechanisms
- HIV/AIDS drug development and treatment
Genomics Research Center, Academia Sinica
2020-2022
McGill University
2006-2022
Academia Sinica
2020
National Chung Hsing University
2020
National Taipei University
2020
Dehydroamino acids are important structural motifs and biosynthetic intermediates for natural products. Many bioactive products of nonribosomal origin contain dehydroamino acids; however, the biosynthesis in most peptides is not well understood. Here, we provide biochemical bioinformatic evidence support role a unique class condensation domains dehydration (CmodAA). We also obtain crystal structure CmodAA domain, which part peptide synthetase AmbE antibiotic methoxyvinylglycine. Biochemical...
Caprazamycin is a nucleoside antibiotic that inhibits phospho-N-acetylmuramyl-pentapeptide translocase (MraY). The biosynthesis of antibiotics has been studied but still far from completion. present study characterized enzymes Cpz10, Cpz15, Cpz27, Mur17, Mur23 out caprazamycin/muraymycin biosynthetic gene cluster, particularly the nonheme αKG-dependent enzyme Cpz10. Cpz15 β-hydroxylase converting uridine mono-phosphate to 5' aldehyde, then incorporating with threonine by Mur17 (Cpz14) form...
20µm) of Functionalized Single Walled Nanotubes (F-SWNT) modified 3501-6 epoxy resin. These nanotubes were dispersed randomly in the before their application laminate. In addition, rheological behavior and another brittle system was characterized using an oscillatory rheometer effect SWNT on resin viscosity is presented. The SWNTs cure thermal degradation epoxies are studied as well.
Phospho-N-acetylmuramoyl-pentapeptide translocase (MraYAA) from Aquifex aeolicus is the binding target for nucleotide antibiotic muraymycin D2 (MD2). MraYAA in presence of MD2 ligand has been crystallized and released, while interactions between active-site residues remain less quantitatively qualitatively defined. We characterized theoretically key involved noncovalent with active site. applied quantum theory atoms molecules natural bond orbital analyses based on density functional method...
Plant type III polyketide synthases produce diverse bioactive molecules with a great medicinal significance to human diseases. Here, we demonstrated versatility of stilbene synthase (STS) from Pinus Sylvestris, which can accept various non-physiological substrates form unnatural products. Three enzymes (4-coumarate CoA ligase, malonyl-CoA synthetase and engineered benzoate ligase) along synthetic chemistry was practiced synthesize starter extender for STS. Of these, the crystal structures...
Kasugamycin (KSM), an aminoglycoside antibiotic, is composed of three chemical moieties: D-chiro-inositol, kasugamine and glycine imine. Despite being discovered more than 50 years ago, the biosynthetic pathway KSM remains unresolved puzzle. Here we report a structural functional analysis for epimerase, KasQ, that primes biosynthesis rather previously proposed KasF/H, which instead acts as acetyltransferase, inactivating KSM. Our biochemical biophysical determined KasQ converts UDP-GlcNAc to...