A5015536585- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- CRISPR and Genetic Engineering
- Biomedical Text Mining and Ontologies
- Bacteriophages and microbial interactions
- Image Retrieval and Classification Techniques
- Antibiotic Resistance in Bacteria
- Plant Virus Research Studies
- Virus-based gene therapy research
- Hemoglobinopathies and Related Disorders
- Biofield Effects and Biophysics
- Prenatal Screening and Diagnostics
- Cancer therapeutics and mechanisms
- Computational Drug Discovery Methods
- RNA Interference and Gene Delivery
- Evolution and Genetic Dynamics
- RNA and protein synthesis mechanisms
- Diverse academic research themes
- Attention Economy in Education and Business
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
Johns Hopkins University
2016-2023
Johns Hopkins Medicine
2018-2023
Center for Systems Biology
2021-2023
High Throughput Biology (United States)
2016-2021
University of New Hampshire at Manchester
2012
Granulomas are a hallmark of tuberculosis. Inside granulomas, the pathogen Mycobacterium tuberculosis may enter metabolically inactive state that is less susceptible to antibiotics. Understanding M. metabolism within granulomas could contribute reducing lengthy treatment required for and provide additional targets new drugs. Two key adaptations nonreplicating phenotype accumulation lipid inclusions in response hypoxic conditions. To explore how these influence granuloma-scale outcomes vivo,...
Abstract Homology Directed Repair (HDR) enables precise genome editing, but the implementation of HDR-based therapies is hindered by limited efficiency in comparison to methods that exploit alternative DNA repair routes, such as Non-Homologous End Joining (NHEJ). In this study, we develop a functional, pooled screening platform identify protein-based reagents improve HDR human hematopoietic stem and progenitor cells (HSPCs). We leverage explore sequence diversity at binding interface NHEJ...
Infection with Mycobacterium avium is a significant cause of morbidity and its treatment requires the use multiple antibiotics for more than 12 months. In current work, we provide genome sequence, gene annotations, ontology protein homology data M. strain 109 (MAC109), which has been used extensively in preclinical studies. The de novo assembled consists circular chromosome length 5,188,883 bp two plasmids sizes 147,100 16,516 bp. We have named pMAC109a pMAC109b, respectively. Based on...
Abstract Mycobacterium avium subsp. hominissuis (MAH) is increasingly recognized as a significant cause of morbidity, particularly in elderly patients or those with immune deficiency underlying lung impairment. Disease due to MAH difficult treat, often requiring years antibiotic therapy. Identification genes essential for growth may lead novel strategies improving curative Here we have generated saturating genome-wide transposon mutant pools strain (MAC109) and developed computational...
The development of antibiotic tolerance is believed to be a major factor in the lengthy duration current tuberculosis therapies. In study, we have modeled vitro by exposing Mycobacterium two distinct stress conditions: progressive hypoxia and nutrient starvation (PBS). We then studied bacterial transcriptional response using RNA-seq employed bioinformatics approach identify important regulators, which was facilitated novel Regulon Enrichment Test (RET). A total 17 transcription regulons were...
The prolonged treatment required to eradicate Mycobacterium avium complex (MAC) infection is likely due the presence of subpopulations antibiotic-tolerant bacteria with reduced susceptibility currently available drugs. However, little known about genes and pathways responsible for antibiotic tolerance in MAC.
Abstract Mycobacterium avium complex (MAC) is one of the most prevalent causes nontuberculous mycobacteria pulmonary infection in United States, yet it remains understudied. Current MAC treatment requires more than a year intermittent to daily combination antibiotic therapy, depending on disease severity. In order shorten and simplify curative regimens, important identify innate bacterial factors contributing reduced susceptibility, namely tolerance genes. this study, we performed...
Abstract Mycobacterium tuberculosis ( Mtb ), the causative agent of (TB), poses a global health challenge and is responsible for over million deaths each year. Current treatment lengthy complex, new, abbreviated regimens are urgently needed. adapts to nutrient starvation, condition experienced during host infection, by shifting its metabolism becoming tolerant killing activity bactericidal antibiotics. An improved understanding mechanisms mediating antibiotic tolerance in can serve as basis...
Mycobacterium tuberculosis (Mtb), the causative agent of (TB), poses a global health challenge and is responsible for over million deaths each year. Current treatment lengthy complex, new, abbreviated regimens are urgently needed. Mtb adapts to nutrient starvation, condition experienced during host infection, by shifting its metabolism becoming tolerant killing activity bactericidal antibiotics. An improved understanding mechanisms mediating antibiotic tolerance in can serve as basis...
Abstract Homology Directed Repair (HDR) enables precise genome editing and holds great promise in the gene therapy field. However, implementation of HDR-based therapies is hindered by limited efficiency comparison to methods that exploit alternative DNA repair routes, such as Non-Homologous End Joining (NHEJ). In this study, we demonstrate development a functional, pooled screening platform utilizing an readout identify protein-based reagents improve HDR outcomes human hematopoietic stem...
Abstract Mycobacterium avium (Mav) is increasingly recognized as a significant cause of morbidity, particularly in elderly patients or those with immune deficiency underlying structural lung disease. Generally, Mav infection treated 2-3 antimicrobial drugs for at least 12 months. Identification genes essential growth may yield novel strategies improving curative therapy. We have generated saturating genome-wide transposon mutant pools commonly used laboratory strain subsp. hominissuis...