A5034879869- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- T-cell and Retrovirus Studies
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- HIV Research and Treatment
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Cervical Cancer and HPV Research
- Cancer-related gene regulation
- Viral-associated cancers and disorders
- Genomics and Phylogenetic Studies
- Turtle Biology and Conservation
- Animal Virus Infections Studies
- Acute Lymphoblastic Leukemia research
- Cancer Genomics and Diagnostics
- Lymphoma Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- RNA Research and Splicing
- Chronic Lymphocytic Leukemia Research
Albert Einstein College of Medicine
2012-2024
Yeshiva University
2002-2019
Montefiore Medical Center
2017-2018
Wellcome Sanger Institute
2010
The Netherlands Cancer Institute
2010
Delft University of Technology
2010
Cancer Genomics Centre
2010
Bipar
2008
University of Central Florida
2008
University of Florida
2008
The sequence of 2,191 nucleotides encoding the env gene murine retrovirus Akv was determined by using a molecular clone provirus. Deduction encoded amino acid showed that single open reading frame encodes 638-amino precursor to gp70 and p15E. In addition, there is typical leader preceding terminus gp70. locations potential glycosylation sites other structural features indicate entire molecule most p15E are located on outer side membrane. Internal cleavage generate occurs immediately adjacent...
Here we show that the tissue specificity of murine retrovirus infections is determined by long terminal repeat (LTR) an otherwise isogenic set viruses. The viruses used for this study contain coding gag, pol, and env genes avirulent Akv virus. Recombinant LTR a virus induces T-cell leukemia lymphoma preferentially infect T lymphocytes. Viruses carry erythroleukemia non-T lymphoblastoid cell lines in marrow spleen. itself displays no preference hematopoietic cells. These experiments suggest...
Human endogenous retroviruses (HERVs) are remnants of ancient infectious agents that have integrated into the human genome. Under normal circumstances, HERVs functionally defective or controlled by host factors. In HIV-1-infected individuals, intracellular defense mechanisms compromised. We hypothesized HIV-1 infection would remove alter controls on HERV activity. Expression could potentially stimulate a T cell response to antigens, and in regions HIV-1/HERV similarity, these cells be...
Transient expression assays were used to determine the sequences within long terminal repeat (LTR) that define high activity in T-lymphoma cells of leukemogenic SL3-3 virus comparison with nonleukemogenic Akv virus. Each these viruses contains related consensus element, enhancer core. The and cores differ at a single base pair. Substitution core element into LTR decreased but not other cell types. Likewise, substitution sequence increased types hematopoietic cells. These data indicate...
Human endogenous retroviruses (HERVs) are transcribed in many cancers including prostate cancer. retrovirus K (HERV-K) of the HML2 subtype is most recently integrated and intact human genome, with viral genomes encoding full- or partial-length proteins. To assess transcripts HERV-K cancer cell lines identify specific elements genome that transcribed, reverse transcriptase-PCR (RT-PCR) cDNA sequencing were undertaken. Strand-specific RT-PCR, plasmid subcloning, detected presence HERV-K(HML2)...
A number of eukaryotlc DNA binding proteins have been Jsolated by screening phage expression libraries with probes containing the site DNA-binding protein. This methodology was employed here to isolate clones factor that interacts W box element human major histocompatibility complex HLA-DQB gene. Surprisingly, several cDNA YB-1, a clone previously isolated CCAAT element-containing sequence were found. Independently, depurinated resulted in isolation YB-1 and dbpA, has homology YB-1....
Marine turtle fibropapillomatosis is associated with chelonid fibropapilloma-associated herpesvirus (C-FP-HV) and commonly affects juvenile green turtles (Chelonia mydas) in neritic (nearshore) habitats. Green have a complex life history, characterized by shifts trophic level as well habitat during ontogeny. Thus, several hypotheses can be proposed for when become infected C-FP-HV. They may acquire the virus at an early stage cycle, including prenatal, hatchling, or posthatchling pelagic...
The identification of tumor-inducing genes is a driving force for elucidating the molecular mechanisms underlying cancer. Many retroviruses induce tumors by insertion viral DNA adjacent to cellular oncogenes, resulting in altered expression and/or structure encoded proteins. availability mouse genome sequence now allows analysis retroviral common integration sites murine be used as genetic screen large numbers candidate cancer genes. By positioning sequences inverse PCR-amplified, virus-host...
Recombination studies have established that retroviral long terminal repeats (LTRs) are important genetic determinants of the viral capacity to induce hematopoietic tumors and specify type cell making up tumor. Plasmids containing LTRs several murine leukemia viruses linked chloramphenicol acetyltransferase gene were tested in transient assays measure relative rates transcriptional activity different types cells. thymomagenic SL3-3, Moloney virus, a mink focus-forming virus all expressed...
We have previously shown that human embryonic stem cells can be differentiated into and fetal type of red blood sequentially express three types hemoglobins recapitulating early erythropoiesis. report here we produced iPS from somatic cell types: adult skin fibroblasts as well mesenchymal cells. show regardless the age donor cells, are fully reprogrammed a pluripotent state is undistinguishable hESCs by low high-throughput expression detailed analysis globin patterns HPLC. This suggests...
Viruses are a leading cause of human morbidity and mortality. Certain viruses, including papillomaviruses (HPVs), play significant role in the etiology cancer. Detection viral DNA insertions genome from next generation sequencing data defines associations with cancer other diseases, identifies impacted organs tissues, provides insights into disease mechanisms has potential to enhance clinical evaluations. In this study, we developed VirusIntegrationFinder (CTAT-VIF), tool for surveying...
Human endogenous retrovirus K (HERV-K) is distinctive among the retroviruses that comprise about 8% of human genome in multiple HERV-K proviruses encode full-length viral proteins, and many formed during recent evolution. gag proteins are found cytoplasm primary tumor cells patients with seminoma. We identified HERV-K-specific T a past history seminoma using interferon-gamma ELISPOT assay an MHC-HERV-K peptide-specific tetramer. A minority apparently healthy subjects without evident germ...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhosphorylated sugars stimulate protein synthesis and Met-tRNAf binding activity in extracts of mammalian cellsJack R. Lenz, Gina E. Chatterjee, Patricia A. Maroney, Corrado BaglioniCite this: Biochemistry 1978, 17, 1, 80–87Publication Date (Print):January 10, 1978Publication History Published online1 May 2002Published inissue 10 January 1978https://pubs.acs.org/doi/10.1021/bi00594a011https://doi.org/10.1021/bi00594a011research-articleACS...
SL3-3 is a leukemogenic, ecotropic retrovirus produced by T-cell line derived from spontaneous lymphoma of an AKR mouse. We have isolated molecular clone its DNA provirus infected NIH 3T3 fibroblasts. Cloned proviral infectious virus upon transfection onto cells. Virus induced lymphomas at high frequency in AKR/J, C3H(f)/Bi, CBA/J, and NFS/N mice. Heteroduplex RNase T1 fingerprinting analyses showed that the genomes non-leukemogenic virus, Akv, contain no major substitutions relative to one...