A5054045582- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Plant-Microbe Interactions and Immunity
- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
- Microbial Natural Products and Biosynthesis
- Plant Disease Resistance and Genetics
- Light effects on plants
- Plant nutrient uptake and metabolism
National University of Rosario
2014-2022
Instituto de Biología Molecular y Celular de Rosario
2014-2022
Consejo Nacional de Investigaciones Científicas y Técnicas
2014-2018
To ensure an adequate organ mass, the daughters of stem cells progress through a transit-amplifying phase displaying rapid cell division cycles before differentiating. Here, we show that Arabidopsis thaliana microRNA miR396 regulates transition root into by interacting with GROWTH-REGULATING FACTORs (GRFs). The GRFs are expressed in but excluded from inhibition miR396. Inactivation increases meristem size and induces periclinal formative divisions cells. repress PLETHORA (PLT) genes,...
Iterative type I polyketide synthases (PKS) are megaenzymes essential to the biosynthesis of an enormously diverse array bioactive natural products. Each PKS contains minimally three functional domains, β‐ketosynthase (KS), acyltransferase (AT), and acyl carrier protein (ACP), a subset reducing domains such as ketoreductase (KR), dehydratase (DH), enoylreductase (ER). The substrate selection, condensation reactions, β‐keto processing growing chain highly controlled in programmed manner....
Microbial lipid production represents a potential alternative feedstock for the biofuel and oleochemical industries. Since Escherichia coli exhibits many genetic, technical, biotechnological advantages over native oleaginous bacteria, we aimed to construct metabolically engineered E. strain capable of accumulating high levels triacylglycerol (TAG) evaluate its neutral productivity during cell density fed-batch fermentations.The Streptomyces coelicolor TAG biosynthesis pathway, defined by...
Microbial synthesis of oleochemicals derived from native fatty acid (FA) metabolism has presented significant advances in recent years. Even so, FA biosynthetic pathways often provide a narrow variety usually linear hydrocarbons, thus yielding end products with limited structural diversity. To overcome this limitation, we took advantage polyketide synthase-based system Mycobacterium tuberculosis and developed an Escherichia coli platform the capacity to synthesize multimethyl-branched...
A broad diversity of natural and non-natural esters have now been made in bacteria, other microorganisms, as a result original metabolic engineering approaches. However, the fact that properties these molecules, therefore their applications, are largely defined by structural features fatty acid alcohol moieties, has driven persistent interest generating novel structures chemicals.In this research, we engineered Escherichia coli to synthesize de novo composed multi-methyl-branched-chain acids...