A5011169296- Liver Disease Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- Legionella and Acanthamoeba research
- Amoebic Infections and Treatments
- Tissue Engineering and Regenerative Medicine
- Liver physiology and pathology
- Hepatitis C virus research
- Parasitic Infections and Diagnostics
- Growth Hormone and Insulin-like Growth Factors
- Vibrio bacteria research studies
- Liver Diseases and Immunity
- Organ Transplantation Techniques and Outcomes
- Biliary and Gastrointestinal Fistulas
- Diagnosis and treatment of tuberculosis
- Liver Disease and Transplantation
- Metabolism, Diabetes, and Cancer
- Heme Oxygenase-1 and Carbon Monoxide
- Diet, Metabolism, and Disease
- Bacterial Infections and Vaccines
- Microbial infections and disease research
- Pancreatitis Pathology and Treatment
- Cancer-related molecular mechanisms research
- Glycogen Storage Diseases and Myoclonus
- Escherichia coli research studies
- Gastric Cancer Management and Outcomes
Universidad Nacional Autónoma de México
2020-2024
Instituto Politécnico Nacional
2012-2024
Hospital General de México
2020-2024
The University of Texas MD Anderson Cancer Center
2023
Universidad Panamericana
2022
Pancreas Centre (Canada)
2021
Motility (United States)
2021
Center for Research and Advanced Studies of the National Polytechnic Institute
2012-2018
Colegio de Postgraduados
2002
It has been 50 years since the first case of primary amoebic meningoencephalitis (PAM), an acute and rapidly fatal disease central nervous system (CNS), was reported in Australia. is now known that aetiological agent PAM Naegleria fowleri, amoeba commonly as 'the brain-eating amoeba'. N. fowleri infects humans different ages who are contact with water contaminated this micro-organism. distributed worldwide found growing bodies freshwater tropical subtropical environments. The number cases...
Respiratory diseases in ruminants are a main cause of economic losses to farmers worldwide. Approximately 25% experience at least one episode respiratory disease during the first year life. Mannheimia haemolytica is etiological bacterial agent ruminant complex. M. can secrete several virulence factors, such as leukotoxin, lipopolysaccharide, and proteases, that be targeted treat infections. At present, little information has been reported on secretion A2 proteases their host protein targets....
Liver disease causes approximately 1.75 million deaths per year and chronic liver (CLD) is usually detected in advanced stages (cirrhosis or hepatocellular carcinoma) that require partial ablation transplant. STAT-3 has been identified as a therapeutic target cancer. Moreover, collagen matrix scaffolds (CMS) can be used carriers of antineoplastic drugs for carcinoma. The objective was to determine the capacity CMS vehicle WP1066 (inhibitor STAT3) an vitro HCC model. incubated with cell lines...
Acanthamoeba castellanii, a free-living amoeba, is an amphizoic organism that can behave as opportunistic pathogen, causing granulomatous amoebic encephalitis in immunocompromised patients or infecting immunocompetent individuals via cutaneous lesions, sinusoidal infections, keratitis. Therefore, this amoeba could be contact with different iron-binding proteins, such lactoferrin tears and mucosa transferrin hemoglobin blood. Iron vital necessary element for host metabolism but also parasite...
Production and activity of matrix metalloproteinases during liver fibrosis progression chronic hepatitis C patients
Naegleria fowleri is a ubiquitous free-living amoeba that causes primary amoebic meningoencephalitis. As part of the innate immune response at mucosal level, proteins lactoferrin (Lf) and lysozyme (Lz) are secreted eliminate various microorganisms. We demonstrate N. survives individual combined effects bovine milk Lf (bLf) chicken egg Lz (cLz). Moreover, proliferation was not altered, even 24 h co-incubation with each protein. Trophozoites’ ultrastructure evaluated using transmission...
Naegleria fowleri is the aetiological agent of primary amoebic meningoencephalitis. This parasite invades its host by penetrating olfactory mucosa. However, mechanism epithelium penetration not well understood. In present study, we evaluated effect N. trophozoites and non-pathogenic gruberi on Madin–Darby canine kidney (MDCK) tight junction proteins, including claudin-1, occludin ZO-1, as actin cytoskeleton. Trophozoites from each free-living amoeba species were co-cultured with MDCK cells...
Naegleria fowleri causes acute and fulminant primary amoebic meningoencephalitis. This microorganism invades its host by penetrating the olfactory mucosa then traveling up mesaxonal spaces crossing cribriform plate; finally, trophozoites invade bulbs. During invasion, protozoan obtains nutrients such as proteins, lipids, carbohydrates, cationic ions (e.g., iron, calcium, sodium) from host. However, mechanism which these are obtained, particularly is poorly understood. In present study, we...
The aim of this work was to identify, characterize and evaluate the pathogenic role mucinolytic activity released by Naegleria fowleri.Zymograms, protease inhibitors, anion exchange chromatography, MALDI-TOF-MS, enzymatic assays, Western blot, confocal microscopy were used identify a secreted mucinase; inhibition assays using antibodies, dot-blots mouse survival tests mucinase as virulence factor.A 94-kDa protein with inducible abolished p-hydroxymercuribenzoate. MALDI-TOF-MS identified...