The usual method to locate and compare loci regulating quantitative traits (QTLs) requires a segregating population of plants with each one genotyped molecular markers. However, from such populations can also be grouped according phenotypic expression trait tested for differences in allele frequency between the bulks: bulk segregant analysis (BSA). same probes used making genetic map (e.g. isozyme, RFLP, RAPD, etc) BSA. A marker showing polymorphism parents which is closely-linked major QTL...

Thyme, mint, and lemon balm were used to determine whether shading conditions could improve the yield, composition, antioxidant, antimicrobial activity in plant essential oils (EOs) comparison with non-shaded plants from an open field. The yield of EOs thyme, balm, was 3.44, 3.96, 0.21 mL/100 g, respectively. Plants covered by nets produced different levels (3.46, 2.20, 0.45 g) after 120 min hydrodistillation. main components thyme oil are thymol (44.2–43.9%), γ-terpinene (18.3–16.8%),...

This study investigated the surface-to-interior mycotoxin contamination of regional speciality meat product 'slavonski kulen' that occurred during a 12-month period and identified moulds present on its surface final production stage. In total, 15 pieces slavonski kulen were produced sampled at months 0, 3, 6, 9 12 (three samples per piece). Concentrations aflatoxin B1 (AFB1) ochratoxin A (OTA) determined using ELISA method, while identification made use both traditional molecular methods....

The presence of ochratoxin A (OTA) was determined in traditional dry-cured meat products made from sub-chronically OTA-exposed pigs. experimental group pigs (n = 5) treated with 300 µg OTA kg(-1) feed during 30 days, whereas the control remained untreated. After household production six types based on recipes, residues were final produced each and untreated animal using an immunoenzymatic technique (ELISA) HPLC fluorescence detection (HPLC-FD). analytical methods showed acceptable...

The aim of this study was to investigate the possibilities ochratoxin A (OTA) reduction in home-made meat products. Meat sausages (n = 50) produced from raw materials coming pigs exposed OTA-contaminated feed, were subject common heat processes practiced households (cooking, frying and baking). Concentrations OTA pre- post-processed products quantified using a validated immunoassay method, enzyme-linked immunosorbent assay, confirmed high-performance liquid chromatography with fluorescence...

Recycling organic waste is most important for preserving natural resources. The research objective was to quantify the effect of application vermicompost and leachate on yield quality strawberries compare it with a standard fertilization program mineral fertilizers during 3-year production cycle. Five treatments were studied: control—without fertilizer (Ø); (V); + foliar (VL); through fertigation (L); NPK (NPK). V positively affected strawberry only in first year. In all three years...

Purpose Quality and top quality wines (wines with geographical indication (GI)) have a larger share (59 per cent) on the Croatian market this was one of reasons for defining subject research. Although there are few research studies, none them is completely oriented role significance GI wine. There an evident lack information in field. This paper aims to address these issues. Design/methodology/approach The population basic group high‐qualified reviewing different aspects wine as complex...

The aim of this article is to investigate the impact casing damaging on formation aflatoxin B1 (AFB1) during ripening dry-fermented meat sausages. level AFB1 contamination was determined in 24 samples using ELISA immunoassay throughout a six-month production period. While with intact no observed whole process, damaged detected end-stages range 1.62-4.49 μg/kg. results showed that occurring long-term sausages can cause contamination, possibly arising grounds diffusion mycotoxin from product...

Digestate is a residue of the anaerobic decomposition organic waste for biogas extraction, but it can be reused as source nutrients. To examine effect digestate in kohlrabi production, field experiments were conducted during three seasons two calendar years. The fertilization treatments included application solid (two rates—DS1 and DS2), liquid rates—DL1 DL2), manure rates—MS1 MS2), mineral fertilizer (NPK) compared with plot without (Ø). results showed significant increase yield use...

In the second half of 20th century awareness importance landraces for future, led to organized collecting missions numerous plant species. A total 2217 maize (Zea mays L.) landraces, collected in former Yugoslavia, are stored at Maize Research Institute (MRIZP) gene bank. During 2014, new were eastern and western parts Macedonia. According site kernel type, 14 samples from Faculty Agricultural Sciences Food, R. Macedonia chosen comparison identification possible duplicates, through coupling...

The yield, grain yield heterosis and genetic distance based on SSR markers were analyzed in eight maize hybrids their parental lines. mean of all F1 was 11.37 tha-1. hybrid from the crosses between L4xL6 gave highest 12.12 For mid parent (MPH) yields hybrids, data showed average value 164.25%, ranged 136.72% (L4xL6) to 218.07% (L8xL2), for better (BPH) 100.70% (H4) 212.60% (H2), averaged 137.36%. among inbred lines 0.58 with a range 0.55 0.61. GD positive correlation (0.22), as well MPH BPH,...

Introduction. The European System for Cardiac Operative Risk Evaluation (EuroSCORE) was developed in order to predict operative risk cardiac surgery and assess the quality of cardio-surgical care. Introduction uniform terminology result evaluation process leads significant improvement measuring surgical treatment quality. Objective. aim study evaluate our results isolated coronary using EuroSCORE. Methods. done respectively by analyzing predicted mortality according EuroSCORE model observed...

Creating new maize hybrids with greater yield potential is a permanent goal of breeding programs all over the world. Long-time existing and problems related to different biotic abiotic stresses growing needs world market require constant work on finding ways for advancing production. Molecular marker technology one fastest developing fields its implementation has already given results in solving improvement. The aim study presented herein was characterization genetic similarity assessment...

The phytate function in plants is still not completely understood: it the primary storage P form seeds that utilized during germination and early seedling development. Approaches to resolve problem of bad nutritive quality grain include engineering crops with reduced levels seed phytic acid. objective this study was investigate genetic variability correlation (Pphy) inorganic phosphorus (Pi) soluble proteins among 28 maize populations, consisted into three groups: low-, intermediate-...

The yield, grain yield heterosis and genetic distance based on SSR markers were analyzed in eight maize hybrids their parental lines. mean of all F1 was 11.37 tha-1. hybrid from the crosses between L4xL6 gave highest 12.12 For mid parent (MPH) yields hybrids, data showed average value 164.25%, ranged 136.72% (L4xL6) to 218.07% (L8xL2), for better (BPH) 100.70% (H4) 212.60% (H2), averaged 137.36%. among inbred lines 0.58 with a range 0.55 0.61. GD positive correlation (0.22), as well MPH BPH,...

Deficiencies of some mineral elements are causing serious health problems, which could be prevented by increase nutrients in food with supplementation, fortification or plant breeding. From this point, experiment 78 maize inbred lines was set up to determine improved contents Fe, Zn and Mn, as well their relations phytic acid, inorganic phosphorus ?-carotene, factors affect absorption. Obtained results suggest that investigated show high variability concentration Mn Zn, acid (which sustain...

Context Biofortification of forage crops has become even more important, due to the improvement in livestock nutrition, but it also had an indirect positive impact on human diet. Aim This study investigated effect nitrogen and microelement (Zn Se) fertilisation yield composition maize (Zea mays L.) silage. Methods Two field experiments were conducted using a two-factorial split-plot design with three doses: 0, 120, 180 240 kg N/ha. The first experiment included foliar Zn as second factor (0,...

Abstract We have described a rare case of Takotsubo cardiomyopathy in pregnancy, which is presented with cardiac arrest (ventricular fibrillation) at onset. In this case, the transient left ventricular ballooning absence coronary artery disease, produced severe impairment function typical echocardiographic and electrocardiographic findings. There were complications form fibrillation, recurrence due to possible new malignant heart rhythm disorder that increases mortality rate compared first...