A5102945553- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Head and Neck Cancer Studies
- Cancer Diagnosis and Treatment
- RNA modifications and cancer
- Cancer-related gene regulation
- Traditional Chinese Medicine Analysis
- Cytokine Signaling Pathways and Interactions
- Tracheal and airway disorders
- Kruppel-like factors research
- Voice and Speech Disorders
- Dysphagia Assessment and Management
- Cancer Research and Treatments
- Congenital Diaphragmatic Hernia Studies
- Ferroptosis and cancer prognosis
- Pharmacological Effects of Medicinal Plants
- Natural product bioactivities and synthesis
- Thyroid Cancer Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- Ginseng Biological Effects and Applications
- Cleft Lip and Palate Research
- Metabolomics and Mass Spectrometry Studies
- Bone Metabolism and Diseases
- Phytochemical Studies and Bioactivities
China Medical University
2010-2021
Tianjin University of Traditional Chinese Medicine
2021
MicroRNA155 plays an important role in many solid malignancies. Expression and function of miR-155 laryngeal carcinoma have not been fully understood. This study aims to investigate the expression squamous cell (LSCC), relationship between its downstream target suppressor cytokine signaling 1 (SOCS1)-STAT3 pathway, related clinicopathological factors. Sixty-three samples twenty-one control mucosa obtained from total laryngectomy cases were analyzed using Western blot analysis real-time PCR....
Plasma miRNAs have been reported as biomarkers for various diseases. In this study, we investigated whether plasma concentrations of miR-126 may be useful laryngeal squamous cell carcinoma (LSCC). We examined the function and mechanism in LSCC by using biology molecular pathology techniques such western blotting, quantitative PCR, IHC IF. The expression Camsap1 mRNA protein is higher cancer tissues compared to that normal tissues. Both were related with prognosis patients. found was able...
Anaplastic thyroid cancer (ATC) is considered to be a rare type of but takes up the most important proportion cancer-related deaths. Therefore, development molecular targeted therapy an exciting strategy in management ATC.miR-155 and SOCS1 expression were measured by qRT-PCR as well western blot analysis. 8305c FRO cells transfected cultured for apoptosis assays, transwell, MTT on miR-155 or suppression overexpression. Dual-luciferase reporter assays restoration experimentswas implemented...
Abstract This study was carried out to evaluate the effects of Astragalus on human nasopharyngeal carcinoma (NPC) viability and apoptosis investigate mechanism in a NPC cell line (CNE2). Cell measured using MTT assay. CNE2 cells treated with were stained acridine orange/ethidium bromide subjected fluorescence microscopy. Bcl‐2, Bax, caspase‐3 ‐8 by western blotting. Rat used establish model. Tumor weight, immune organ index T lymphocyte subsets employed detect immunoregulatory antitumor...
Laryngeal squamous cell carcinoma (LSCC) is one of the most common head and neck malignancies with a poor prognosis. The aim this study was to detect expression microRNAs (miRNAs) in supraglottic LSCC attached normal mucosa using microarray assay, explore role miRNAs LSCC. Fresh-frozen samples five were obtained, their various endogenous screened by microarray. interest confirmed quantitative real-time polymerase chain reaction (qRT-PCR) 48 tumors 10 tissues. In total, 38 differentially...
We sought to determine the function of miR-181a/GATA6 pathway in progression laryngeal squamous cell carcinoma (LSCC).The expression miR-181a and GATA6 were detected using quantitative real-time-PCR western blotting 127 LSCC samples 32 corresponding control mucosa tissues. Cell death, migration apoptosis measured Hep-2 cells 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), Transwell assay assay, respectively. The prognosis was determined by follow-up, univariate...
Introduction: Each year, ~50,000 patients worldwide die of laryngeal squamous cell carcinoma (LSCC) because its highly metastatic properties. However, pathogenic mechanisms are still unclear, and in particular, the prediction metastasis remains elusive. This study aimed to define role microRNA-145 (miR-145) LSCC progression. We also elucidate clinical significance miR-145/MYO5A pathway, especially predictive function MYO5A neck lymph node metastasis. Materials methods: miR-145 expression was...
In the present study, we investigated effects of p21-activated kinase 4 (Pak4) gene on Hep-2 laryngeal carcinoma cells in vivo and vitro. The expression Pak4 was downregulated using small interfering RNA (siRNA). downregulation decreased proliferation increased apoptosis S phase arrest further experiments, determined that S/G(2) transition obstructed by 5‑chloro-2'‑deoxyuridine (CldU) 5‑iodo‑2'‑deoxyuridine (IdU) double staining. A xenografted tumor mouse model created inducing human tumors...
Papillary thyroid carcinoma (PTC) accounts for the largest proportion of cancers; and its morbidity rate has dramatically increased in recent decades.However, pathogenesis mechanisms PTC are still not clear.This study aimed to reveal that miR-145 acts as an antitumor miRNA progression PTC.In present study, expression was analyzed 57 paired patient samples.The relationship between clinicopathological features were also defined.The tumor suppressive function on cell metastasis, proliferation...
Our purpose was to develop and verify an immune-related signature for predicting recurrence risk of patients with laryngeal cancer.RNA-seq data 51 81 non-recurrence cancer samples were downloaded from TCGA database, as the training set. Microarray 34 75 obtained GEO dataset, validation Single factor cox regression utilized screen prognosis-related immune genes. After LASSO analysis, constructed. Recurrence free survival (RFS) between high- low- presented, followed by ROC. We also evaluated...
Seven undescribed cembranoids, sacraoxides A–G ( 1, 3 – 8 ) were isolated from the gum resin of Boswellia sacra . Their structures elucidated by extensive physicochemical and spectroscopic analysis, as well ECD calculation, modified Mosher’s method X-ray diffraction crystallography. Compounds 6 7 exhibited inhibitory activities on nitric oxide (NO) production induced lipopolysaccharide in RAW264.7 cells with IC 50 values 24.9 ± 1.7 36.4 2.9 μ M.
Down-regulation of metastasis inhibitor genes PTEN and thrombospondin 2 may play a role in the lymphatic supraglottic carcinoma.To investigate differentially expressed squamous cell carcinoma with without using cDNA microarray.cDNA microarray was used to detect expression profiles results were validated real-time PCR.Compared normal laryngeal tissues, 15.6% (15/96) including cathepsin B, D, L, Ets1, c-Ets2, PEA3 up-regulated both cases carcinoma. Compared no lymph node metastasis,...
Long noncoding RNA (lncRNA) plays a critical role in tumorigenesis. How lncRNA regulates laryngeal squamous cell carcinoma (LSCC) progression remains poorly understood. In the present study, we found that LINC01638 was highly expressed LSCC tissues. And expression positively correlated with clinical stage and lymph node metastasis. Patients high displayed low survival rate. Results from CCK8, colony formation, transwell assays showed knockdown suppressed proliferation, migration invasion of...
Objective:To assess the efficacy of partial CO₂ laser arytenoidectomy in cases with bilateral vocal fold paralysis. Method:A total number 11 patients paralysis who undergone was included this retrospective study. The treatment evaluated by compare form glottis, scale dyspnea and change voice preoperatively postoperatively, as well occurrence surgery complications such dysphonia dysphagia. Result:①All showed alleviation had endotracheal tube removed successfully 1-2 months after surgery. ②All...